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| 1 | m TOR signaling in liver regeneration: Rapamycin combined with growth factor treatment显示文摘AIM: To investigate the effects of mammalian target of rapamycin(mT OR) inhibition on liver regeneration and autophagy in a surgical resection model.METHODS: C57BL/6 mice were subjected to a 70% partial hepatectomy(PH) and treated intraperitoneally every 24 h with a combination of the m TOR inhibitor rapamycin(2.5 mg/kg per day) and the steroid dexamethasone(2.0 mg/kg per day) in phosphate bufferedsaline(PBS) or with PBS alone as vehicle control. In the immunosuppressant group, part of the group was treated subcutaneously 4 h prior to and 24 h after PH with a combination of human recombinant interleukin 6(IL-6; 500 μg/kg per day) and hepatocyte growth factor(HGF; 100 μg/kg per day) in PBS. Animals were sacrificed 2, 3 or 5 d after PH and liver tissue and blood were collected for further analysis. Immunohistochemical staining for 5-Bromo-2'-deoxyuridine(Brd U) was used to quantify hepatocyte proliferation. Western blotting was used to detect hepatic microtubule-associated protein 1 light chain 3(LC3)-Ⅱ protein expression as a marker for autophagy. Hepatic gene expression levels of proliferation-, inflammation- and angiogenesisrelated genes were examined by real-time reverse transcription-polymerase chain reaction and serum bilirubin and transaminase levels were analyzed at the clinical chemical core facility of the Erasmus MC-University Medical Center.RESULTS: m TOR inhibition significantly suppressed regeneration, shown by decreased hepatocyte proliferation(2% vs 12% Brd U positive hepatocyte nuclei at day 2, P < 0.01; 0.8% vs 1.4% at day 5, P = 0.02) and liver weight reconstitution(63% vs 76% of initial total liver weight at day 3, P = 0.04), and furthermore increased serum transaminase levels(aspartate aminotransferase 641 U/L vs 185 U/L at day 2, P = 0.02). Expression of the autophagy marker LC3-Ⅱ, which was reduced during normal liver regeneration, increased after mT OR inhibition(46% increase at day 2, P = 0.04). Hepatic gene expression showed an increased inflammation-related response [tumor necrosis factor(TNF)-α 3.2-fold upregulation at day 2, P = 0.03; IL-1Ra 6.0-fold upregulation at day 2 and 42.3-fold upregulation at day 5, P < 0.01] and a reduced expression of cell cycle progression and angiogenesis-related factors(HGF 40% reduction at day 2; vascular endothelial growth factor receptor 2 50% reduction at days 2 and 5; angiopoietin 1 60% reduction at day 2, all P ≤ 0.01). Treatmentwith the regeneration stimulating cytokine IL-6 and growth factor HGF could overcome the inhibitory effect on liver weight(75% of initial total liver weight at day 3, P = 0.02 vs immunosuppression alone and P = 0.90 vs controls) and partially reversed gene expression changes caused by rapamycin(TNF-α and IL-1Ra levels at day 2 were restored to control levels). However, no significant changes in hepatocyte proliferation, serum injury markers or autophagy were found.CONCLUSION: mT OR inhibition severely impairs liver regeneration and increases autophagy after PH. These effects are partly reversed by stimulation of the IL-6 and HGF pathways. | Suomi MG Fouraschen Petra E de Ruiter Jaap Kwekkeboom Ron WF de Bruin Geert Kazemier Herold J Metselaar Hugo W Tilanus Luc JW van der Laan Jeroen de Jonge | 2013 | World Journal of Transplantation2013，3，3： | 6 |
| 2 | Soil biodiversity for agricultural sustainability显示文摘 | Lijbert Brussaard Peter C. de Ruiter George G. Brown | 2006 | Agriculture, Ecosystems and Environment2006，，3： | 3 |
| 3 | 应用ICF扩大慢性神经性疾病中健康问题范围的研究显示文摘目的确定能够显示慢性神经功能障碍(多发性硬化症、帕金森氏病和神经肌肉疾病)患者有关健康问题的ICF类目。方法采用Delphi研究方法,由三方面与疾病相关的人组成专家小组,即患者及其代理人、医疗和非医疗卫生专业人士(n=98)。小组要求根据《国际功能,残疾和健康分类》(ICF)反映有关特定疾病的健康问题,选择相关类目。小组成员将这些类目与简明残疾记录量表(MRD)和残疾及其影响量表(DIP)两种测量工具中的指标作比较。结果选择出68项ICF类目相关性最大,分别属于四个ICF领域。患者/代理人和卫生专业人员两者评价的类目无显著性差异。疾病小组的一致性很高。三个特定疾病小组的选择在'身体功能和结构'领域存在差异,但如果扩大纳入标准则可以达成共识。所选择的ICF项目涵盖了几乎所有测量项目。最大的差异表现在'参与'和'环境因素'领域选择的类目。结论与广泛应用于神经学领域的健康状态测量相比,在研究健康问题时所选择的ICF类目更为广泛,特别是涉及ICF的'参与'和'环境因素'领域时。 | Klaske WYNIA Berrie MIDDEL Jitse P. van DIJK Han de RUITER Willem LOK Jacques Ha de KEYSER Sijmen A. REIJNEVELD 王朴(译) 张君梅(译) 荀芳(译) 邱卓英(审校) 李建军(审校) | 2008 | 中国康复理论与实践2008，14，12： | 2 |
| 4 | Regulated expression of the inhibitory receptor LAIR-1 on human peripheral T cells during T cell activation and differentiation显示文摘 | Jansen CA Cruijsen CW de Ruiter T | 2007 | J Immunol2007，37，4： | 1 |
| 5 | Anatomical basis of autonomic nerve-preserving total nesorectal excision for rectal cancer 显示文摘 | Havenga K De Ruiter MC Enker WE | 1996 | Br J Surg1996，83，4： | 1 |
| 6 | Development of the cardiac conduction tissue in human embryos using HNK-1 antigen expression:Possible relevant for understanding of abnormal atrial automaticity显示文摘 | Gittaenberger-De Groot AC De Ruiter MC | 1999 | Circulation1999，99，6： | 1 |
| 7 | Changes in phototaxis during the larval life of the eyed hawk moth显示文摘 | DE Ruiter VAN DER Horn I J | 1957 | Nature1957，179，： | 1 |
| 8 | Direct control of the Forkhead transcription factor AFX by protein kinase B显示文摘 | Kops G J de Ruiter ND de Vries-Smits AM | 1999 | Nature1999，398，6728： | 1 |
| 9 | Management of ob- structive sleep apnea in edentulous patients: an overview of the literature显示文摘 | Heidsieck DS de Ruiter MH de Lange J | 2016 | Sleep Breath2016，20，1： | 1 |
| 10 | RNA interference by expression of short interfering RNAs and hairpin RNAs in mammalian cells 显示文摘 | Yu J Y De Ruiter S L Turner D L | 2002 | Proc Natl Acad Sci USA2002，99，9： | 1 |
| 11 | Development and degeneration of retina in rds mutant mice∶ light microscopy显示文摘 | De Ruiter A Hawkins RK | 1980 | J Comp Neurol1980，194，： | 1 |
| 12 | Treatmenl of complexbillaD' stones by eholangioseopy laser litho/ripsy in 10 patients显示文摘 | Verdonk RC de Ruiter A J Weersma RK | 2010 | Ned TijdschrGeneeskd2010，154，： | 1 |
| 13 | A power analysis for future clinical trials on the potential adverse effects of SSRIs on amygdala reactivity显示文摘有抗抑郁剂的青少年的处理可以在这张人口为 suicidality 导致增加的风险。杏仁之类的活动在与功能的磁性的回声成像(fMRI ) 感情的脸处理期间是感情的 dysregulation 的一项著名措施。与 fluoxetine ( NTR3103 )在我们的过早地结束的使随机化的临床的试用的数据之上基于在焦急并且或我们计算了的沮丧的女孩( 12-14 岁) r =与发现效果缩放 0.66 与安慰剂相比,仅仅 8 个题目被需要活动追随者 16 星期处理与 fluoxetine 表明增加的杏仁之类。 | M. A. Bottelier A. Schrantee G. van Wingen H. G. Ruhe M. B. de Ruiter L. Reneman | 2016 | Frontiers in Biology2016，11，3： | 1 |
| 14 | Soil biodiversity for agricultural sustainability显示文摘 | Brussaard L de Ruiter P C Brown G G | 2007 | Agriculture Ecosystems an Environment2007，121，： | 1 |
| 15 | Differential expression of leukocyte-associated Ig-like receptor-1 during neutrophil differentiation and activation显示文摘 | Verbrugge A de Ruiter T Geest C | 2006 | J Leukoc Biol2006，79，4： | 1 |
| 16 | Collagens are functional, high affinity ligands for the inhibitory immune receptor LAIR-1 显示文摘 | Lebbink R J de Ruiter T Adelmeijer J | 2006 | J Exp Med2006，203，6： | 1 |
| 17 | Isotopic evidence for dietary variability in the early Hominin Paranthropus robustus显示文摘 | Sponheimer M Passey B H de Ruiter D J | 2006 | Science2006，314，10： | 1 |
| 18 | The CICERO system for CAD/CAM fabrication of full-ceramic crowns显示文摘 | Van der Zel JM Vlaar S de Ruiter WJ | 2001 | J Prosthet Dent2001，85，3： | 1 |
| 19 | RNA interference hy ex- pression of short-interfering RNAs and hairpin RNAs in mammalian cells 显示文摘 | Yu JY De Ruiter SL Turner DL | 2002 | Proc Natl Acad Sci USA2002，99，9： | 1 |
| 20 | Anatomical basis of autonomic nerve-preserving total mesorectal excision for rectal cancer显示文摘 | HAVENGA K DE RUITER MC ENKER WE | 1996 | Br J Surg1996，83，4： | 1 |
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