|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Studies of biouptake and transformation of mercury by a typical unicellular diatom Phaeodactylum tricornutum显示文摘Mercury(Hg) is a toxic heavy metal with its biogeochemical cycling in the ocean depending on the type and behavior of the oceanic microalgae.The present work aimed to evaluate bioaccumulation and transformation of Hg by Phaeodactylum tricornutum,a typical unicellular diatom,when exposed to the extremely high level of Hg in order to understand the possible mechanisms of acute stress response.P.tricornutum can accumulate Hg(its bioaccumulation factor is at 104 level),and the 96 h EC 50 was estimated to be 145μg L-1.The amounts of surface-bound Hg being about 1.2 to 4.8 times higher than those of intracellular Hg under exposure to HgCl 2(from 20 to 120μg L-1 concentrations) suggested that the cell wall of P.tricornutum is an important 'fence' towards Hg.After entering the P.tricornutum cell,Hg underwent transformation in its chemical form via interactions with high molecular weight sulfur-containing proteins(accounting for 68% of the intracellular Hg),and glutathione as well as the induced phytochelatins(PCs)(24% Hg) which alleviated the toxicity of HgCl2.In addition,the existence of organic ligands greatly influenced the uptake and transformation behavior of P.tricornutum towards HgCl2,especially in the case of cysteine(Cys),which increased the uptake of Hg,but alleviated the toxicity of Hg towards P.tricornutum due to the fact that Cys is an important precursor for the synthesis of PCs inside the cell.The uptake process of Hg by P.tricornutum was in agreement with the Freundlich isotherm,suggesting a typical heterogeneous sorption process.More importantly,we observed the conversion of HgCl2 into methylmercury inside the P.tricornutum cells and its release into the culture solution using HPLC/CVG-AFS and GC-MS,although the mechanism needs to be further investigated. | DENG GuiFu ZHANG TianWen YANG LiMin WANG QiuQuan | 2013 | Chinese Science Bulletin2013，58，2： | 7 |
| 2 | Mutation breeding by ion implantation显示文摘 | Yu Zengliang Deng Jianguo He Jianjun HuoYuping Wu Yuejin Wang Xuedong Lui Guifu | 1991 | Nuclear Instruments and Methods in Physics Research1991，5960，： | 1 |
| 3 | Room tem- perature mass production of carbon nanotube field emission micro -cathode arrays using electropla- ting of a CNT/Ni composite followed by micro - machining 显示文摘 | Min Deng Guifu Ding Yan Wang | 2011 | Vacuum2011，，85： | 1 |
| 4 | Fabrication of Ni-matrix car- bon nanotube field emitters using composite electroplating and micromachining 显示文摘 | Deng Min Ding Guifu Wang Yan | 2009 | Carbon2009，47，： | 1 |
| 5 | Identification of a candidate QTG for seed number per silique by integrating QTL mapping and RNA-seq in Brassica napus L.显示文摘Seed number per silique(SNPS)is one of seed yield components in rapeseed,but its genetic mechanism remains elusive.Here a double haploid(DH)population derived from a hybrid between female 6Q006with 35–40 SNPS and male 6W26 with 10–15 SNPS was investigated for SNPS in the year 2017,2018,2019 and 2021,and genotyped with Brassica 60K Illumina Infinium SNP array.An overlapping major QTL(qSNPS.C09)explaining 51.50%of phenotypic variance on average was narrowed to a 0.90 Mb region from 44.87 Mb to 45.77 Mb on chromosome C09 by BSA-seq.Subsequently,two DEGs in this interval were detected between extreme individuals in DH and F_2populations by transcriptome sequencing at7 and 14 days after pollination siliques.Of which,BnaC09g45400D encoded an adenine phosphoribosyltransferase 5(APT5)has a 48-bp InDel variation in the promoter of two parents.Candidate gene association analysis showed that this InDel variation was associated with SNPS in a nature population of rapeseed,where 54 accessions carrying the same haplotype as parent 6Q006 had higher SNPS than103 accessions carrying the same haplotype as parent 6W26.Collectively,the findings are helpful for rapeseed molecular breeding of SNPS,and provide new insight into the genetic and molecular mechanism of SNPS in rapeseed. | Shuangshuang Xin Hongli Dong Yixin Cui Yilin Liu Guifu Tian Nanxi Deng Huafang Wan Zhi Liu Xiaorong Li Wei Qian | 2023 | The Crop Journal2023，11，1： | 0 |
| 6 | Improved Non-negative Matrix Factorization Algorithm for Sparse Graph Regularization显示文摘Aiming at the low recognition accuracy of non-negative matrix factorization(NMF)in practical application,an improved spare graph NMF(New-SGNMF)is proposed in this paper.New-SGNMF makes full use of the inherent geometric structure of image data to optimize the basis matrix in two steps.A threshold value s was first set to judge the threshold value of the decomposed base matrix to filter the redundant information in the data.Using L2 norm,sparse constraints were then implemented on the basis matrix,and integrated into the objective function to obtain the objective function of New-SGNMF.In addition,the derivation process of the algorithm and the convergence analysis of the algorithm were given.The experimental results on COIL20,PIE-pose09 and YaleB database show that compared with K-means,PCA,NMF and other algorithms,the proposed algorithm has higher accuracy and normalized mutual information. | Caifeng Yang Tao Liu Guifu Lu Zhenxin Wang Zhi Deng | 2021 | 国际计算机前沿大会会议论文集2021，，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员