|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants显示文摘Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated perennial crop plants is challenging but highly desirable.Here,we report a highly useful method using an Agrobacterium-mediated transient CRISPR/Cas9 gene expression system to create non-transgenic mutant plants without the need for sexual segregation.We have also developed a rapid,cost-effective,and high-throughput mutant screening protocol based on Illumina sequencing followed by high-resolution melting(HRM)analysis.Using tetraploid tobacco as a model species and the phytoene desaturase(PDS)gene as a target,we successfully created and expediently identified mutant plants,which were verified as tetra-allelic mutants.We produced pds mutant shoots at a rate of 47.5%from tobacco leaf explants,without the use of antibiotic selection.Among these pds plants,17.2%were confirmed to be non-transgenic,for an overall non-transgenic mutation rate of 8.2%.Our method is reliable and effective in creating non-transgenic mutant plants without the need to segregate out transgenes through sexual reproduction.This method should be applicable to many economically important,heterozygous,perennial crop species that are more difficult to regenerate. | Longzheng Chen Wei Li Lorenzo Katin-Grazzini Jing Ding Xianbin Gu Yanjun Li Tingting Gu Ren Wang Xinchun Lin Ziniu Deng Richard J.McAvoy Frederick G.Gmitter Jr. Zhanao Deng Yunde Zhao Yi Li | 2018 | Horticulture Research2018，5，1： | 12 |
| 2 | A Remark on the Mallat Pyramidal Algorithm of Wavelet Analysis显示文摘The exact relationships between the lenthgs of scale sequences and wavelet sequences in the Mallat pyramidal algorithm for computing wavelet trans- form coefficients are obtained,and the maximum possible scale of arbitrary discrete signal is derived. | Xianbin LI &1 Houqiang LI &2 Fuquan WANG &3Jing DING 1 (Dept.of Physics,Sichuan Union University,Chengdu6 1 0 0 6 4,P.R.China) 2 (Dept.of Math.Sichuan teacher’s College,Nanchong6 370 0 2 P.R.China) 3(Dept.of Hydrology,Sichuan Union University.Chengdu6 | 1997 | Communications in Nonlinear Science and Numerical Simulation1997，2，4： | 3 |
| 3 | Predictors of HIV and syphilis among men who have sexwith men in a Chinese metropolitan city:comparison ofrisks among students and non-students显示文摘 | ZHANG Lan DING Xianbin LU Rongrong | 2012 | PLOS ONE2012，7，37： | 1 |
| 4 | First Total Synthesis of a Fluorescent Didemnin 显示文摘 | Padma Portonovo Xianbin Ding Michael S Leonard | 2000 | Tetrahedron2000，56，： | 1 |
| 5 | High HIV prevalence detected in 2006 and 2007 among men who have sex with men in China's largest municipality:an alarming epidemic in Chongqing,China显示文摘 | Feng L Ding Xianbin Lu Rongrong | | 0，，01： | 1 |
| 6 | Evaluating Behavioral Risk Factor Interventions for Hypertensive and Diabetic Patient Management in the National Basic Public Health Service Programs from 2009显示文摘BACKGROUND In recent decades,China has faced a double burden of infectious diseases and noncommunicable diseases(NCDs).The number of deaths due to NCDs accounts for 88.5%of total deaths in China in 2019.To address these public health issues and improve the accessibility and affordability of primary health care,a National Basic Public Health Service Program(NBPHSP)was introduced as part of China’s healthcare reform initiated in 2009.Subsided by national and local government budgets,NBPHSP is mainly implemented by community and township health service centers,as well as health stations in urban areas and village clinics in rural areas(1).Health management of patients with hypertension and diabetes is an integral and pivotal part of the NBPHSP. | Liuxia Yan Ning Ji Jian Xu Min Liu Lizheng Guan Kejun Liu Ainan Jia Xianbin Ding Dan Liu Man Li Yanfang Zhao Shengquan Mi Wenhua Zhao Zhuoqun Wang Yamin Bai | 2022 | China CDC weekly2022，4，19： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员