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| 1 | p16 promoter hypermethylation:A useful serum marker for early detection of gastric cancer显示文摘AIM: To determine p16 promoter hypermethylation in gastric tumoral tissue and serum samples, its impact on p16-protein expression, and correlation with clinical and histological features. METHODS: Samples were obtained from 52 histologically confirmed cases of gastric adenocarcinoma. Gastric tissue and serum of 50 age- and sex-matched individuals with normal gastroscopy and biopsy were obtained as control samples. Methylation-specific polymerase chain reaction (MSP) was used to evaluate methylation status of p16 promoter. p16-protein expression was analyzed by immunohistochemical staining on paraffin-embedded sections. RESULTS: Methylation was detected in 44.2% (23/52) of tumoral tissues. 60.9% of them were also methylated in serum, i.e., 26.9% of all patients (14/52). Methylation was not detected in tissue and sera of control samples. p16-protein expression was decreased in 61.5% of cases (32/52), and was significantly associated with promoter hypermethylation (P < 0.001). Methylation was significantly more frequent in higher pathological grades (P < 0.05). Methylation was not associated with other clinicopathological features and environmental factors including H pylori infection and smoking. CONCLUSION: p16 promoter hypermethylation is an important event in gastric carcinogenesis. It is the principle mechanism of p16 gene silencing. It is related to malignant tumor behavior. Detection of DNA methylation in serum may be a biomarker for early detection of gastric cancer. | Mohammad Reza Abbaszadegan Omeed Moaven Hamid Reza Sima Kamran Ghafarzadegan Azadeh A'rabi Mohammad Naser Forghani Hamid Reza Raziee Ali Mashhadinejad Mostafa Jafarzadeh Ehsan Esmaili-Shandiz Ezzat Dadkhah | 2008 | World Journal of Gastroenterology2008，14，13： | 50 |
| 2 | Stool-based DNA testing,a new noninvasive method for colorectal cancer screening,the first report from Iran显示文摘AIM:To detect tumor-associated DNA changes in stool samples among Iranian patients with colorectal cancer(CRC)compared to healthy individuals using BAT-26,p16 hypermethylation and long DNA markers.METHODS:Stool DNA was isolated from 45 subjects including 25 CRC patients and 20 healthy individuals using a new,fast and easy extraction method.Long DNA associated with tumor was detected using polymerase chain reaction method.Microsatellite studies were performed utilizing denaturating polyacrylamide gel to determine the instability of BAT-26.Methylation status of p16 promoter was analyzed using methylation-specific PCR(MSP).RESULTS:The results showed a significant difference in existence of long DNA(16 in patients vs 1 in controls,P < 0.001)and p16(5 in patients vs none in controls,P = 0.043)in the stool samples of two groups.Long DNA was detected in 64% of CRC patients;whereas just one of the healthy individuals was positive for Long DNA.p16 methylation was found in 20% of patients and in none of healthy individuals.Instability of BAT-26 was not detected in any of stool samples.CONCLUSION:We could detect colorectal cancer related genetic alterations by analyzing stool DNA witha sensitivity of 64% and 20% and a specificity of 95% and 100% for Long DNA and p16 respectively.A non-invasive molecular stool-based DNA testing can provide a screening strategy in high-risk individuals.However,additional testing on more samples is necessary from Iranian subjects to determine the exact specificity and sensitivity of these markers. | Mohammad Reza Abbaszadegan Alireza Tavasoli Arash Velayati Hamid Reza Sima Hassan Vosooghinia Mehdi Farzadnia Hamid Asadzedeh Mehran Gholamin Ezzat Dadkhah Azadeh Aarabi | 2007 | World Journal of Gastroenterology2007，13，10： | 14 |
| 3 | A Comprehensive Overview on the Latest Progress in the Additive Manufacturing of Metal Matrix Composites: Potential, Challenges, and Feasible Solutions显示文摘Nowadays, as an emerging technology, additive manufacturing(AM) has received numerous attentions from researchers around the world. The method comprises layer-by-layer manufacturing of products according to the 3D CAD models of the objects. Among other things, AM is capable of producing metal matrix composites(MMCs). Hence, plenty of works in the literature are dedicated to developing different types of MMCs through AM processes. Hence, this paper provides a comprehensive overview on the latest research that has been carried out on the development of the powder-based AM manufactured MMCs from a scientific and technological viewpoint, aimed at highlighting the opportunities and challenges of this innovative manufacturing process. For instance, it is documented that AM is not only able to resolve the reinforcement/matrix bonding issues usually faced with during conventional manufacturing of MMCs, but also it is capable of producing functionally graded composites and geometrically complex objects. Furthermore, it provides the opportunity for a uniform distribution of the reinforcing phase in the metallic matrix and is able to produce composites using refractory metals thanks to the local heat source employed in the method. Despite the aforementioned advantages, there are still some challenges needing more attention from the researchers. Rapid cooling nature of the process, significantly different coe fficient of expansion of the matrix and reinforcement, processability, and the lack of suitable parameters and standards for the production of defect-free AM MMCs seem to be among the most important issues to deal with in future works. | Mehran Dadkhah Mohammad Hossein Mosallanejad Luca Iuliano Abdollah Saboori | 2021 | Acta Metallurgica Sinica(English Letters)2021，34，9： | 2 |
| 4 | Role of hMLH1 and E-Cadherin Promoter Methylation in Gastric Cancer Progression显示文摘 | Meysam Moghbeli Omeed Moaven Bahram Memar Hamid Reza Raziei Azadeh Aarabi Ezzat Dadkhah Mohammad Mahdi Forghanifard Fatemeh Manzari Mohammad Reza Abbaszadegan | 2014 | Journal of Gastrointestinal Cancer2014，，1： | 2 |
| 5 | Study of body image in fertile and infertile men 显示文摘 | Akhondi MM Dadkhah A Bagherpour A | 2011 | J Re- prod Infertil2011，12，4： | 1 |
| 6 | Correlations between crude oil composition and produced water quulity:A multivariate analysis approach显示文摘 | Eftekhar Dadkhah M qye G | 2013 | Ind Eng Chem Res2013，52，17： | 1 |
| 7 | The macroscopic elasticity of 3Dwoven composites显示文摘 | Cox BN Dadkhah MS | 1995 | J Compos Mater1995，9，6： | 1 |
| 8 | Colloids and surfaces a: physicochem显示文摘 | AMIRI M C DADKHAH A | 2006 | Eng Aspects2006，278，： | 1 |
| 9 | Inhibitory effects of dietary Caraway essential oils on 1,2-dimethylhydrazine-induced colon carcinogenesis is mediated by liver xenobiotic metabolizing enzymes 显示文摘 | Dadkhah A Allameh A Khalafi H | 2011 | Nutrition and Cancer2011，63，1： | 1 |
| 10 | On the tensile failureof 3D woven composites显示文摘 | COX B N DADKHAH M S MORRIS W L | 1996 | Composites Part A:AppliedScience and Manufacturing1996，27，6： | 1 |
| 11 | Convenient method for preparation of hydrophobically modified starch nanocrystals with using fatty acids显示文摘 | Hassan Namazi Abbas Dadkhah | 2009 | Carbohydrate Polymers2009，，3： | 1 |
| 12 | Association of helicobacter pylori infection with severity of coronary heart disease显示文摘 | Rogha M Dadkhah D Pourmoghaddas Z | 2012 | ARYA Atheroscler2012，7，4： | 1 |
| 13 | Evaluation and comparison of SYBR Green I Real-Time PCR and TaqMan Real-Time PCR methods for quantitative assay of Listeria monocytogenes in nutrient broth and milk 显示文摘 | Hassan Dadkhah Mohammad Reza Bassami Saeed Hashemi | 2012 | African Journal of Microbiology Research2012，6，9： | 1 |
| 14 | On the tensile failure of 3D woven composites 显示文摘 | COX B N DADKHAH M S MORRIS W L | 1996 | Compos A Appl Sci Manuf1996，27，6： | 1 |
| 15 | Benign retroperitoneal schwannoma mimicking adrenal mass 显示文摘 | DADKHAH F SALIMI M KAVIANI A | 2005 | Urol J2005，2，1： | 1 |
| 16 | Mechanisms of compaction failure 3D composites 显示文摘 | COX B N DADKHAH M S INMAN R V | 1992 | Acta Metall Mater1992，40，12： | 1 |
| 17 | Cumulant based stochastic reactive power planning method for distribution systems with wind generators显示文摘 | Dadkhah M Venkatesh B | 2012 | IEEE Transactions on Power Systems2012，27，4： | 1 |
| 18 | On the tensile failure of 3d woven composites显示文摘 | COX B N DADKHAH M S MORRIS W L | 1996 | Composites Part a-Applied Science and Manufacturing1996，27，6： | 1 |
| 19 | Evaluation and comparison of SYBR Green Ⅰ real-time PCR and TaqMan real-time PCR methods for quantitative assay of Listeria monocytogenes in nutrient broth and milk 显示文摘 | Dadkhah H Bassami M R Hashemi S | 2012 | African Journal of Microbiology Research2012，6，9： | 1 |
| 20 | Dynamic Crack Curving and Branching under Biaxial Loading显示文摘 | HAWONG J S KOBAYASHI A S DADKHAH M S | 1987 | Experimental Mechanics1987，27，2： | 1 |
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