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| 1 | Gene expression profiling under different photoperiod/temperature conditions in a photoperiod-/thermo-sensitive genic male sterile line of rice(Oryza sativa L.)显示文摘Peiai 64S is a photoperiod- and thermo- sensitive genic male sterile (PTSGMS) line of rice, which is male sterile at long day/high temperature and partial fertile at short day/low temperature. A cDNA array representing 3328 unique rice genes was used to profile the gene expression patterns in the young panicles of Peiai 64S under these two condi- tions. The statistical data showed that up to 14.60% of genes exhibited up-or down-regulated expressions in the plants at long day/high temperature compared with plants at short day/low temperature. Only four genes were up-regulated while 482 genes down-regulated. Real-time PCR with all the up-regulated and 9 randomly selected down-regulated genes confirmed the differential expressions detected by the array, indicating that the constructed cDNA array is reliable. These differently expressed genes participated in almost all cell biological responses. Analysis of up- and down-regulated genes revealed distinctive changes between the mRNA abundances of MMK1 and MMK2, both of which are analogs ofMAPK, and significant down-regulation of several transcription factors. It was suggested that changes that occurred in the MAPK signal transduction path- way might disturb the transcription control necessary for morphogenesis of pollens and corresponding phys- iological functions. | GUO Xiaoqin DONG Haitao ZHENG Kangle LUO Hongmei TAN Xuelin FANG Yongqi WANG Yanqin DENG Ye DAI Chengen LOU Yichun SHAO Jing SHI Wenqin ZHAO Dong LI Debao | 2006 | Chinese Science Bulletin2006，51，2： | 2 |
| 2 | Frame of AGO generating space显示文摘 | Dai Debao Chen Rongqiu Zhang Shuhong | 2001 | The Journal of Grey System2001，，1： | 1 |
| 3 | Frame of AGO Generating Space显示文摘 | Dai Debao Chen Rongqiu | 2001 | The Journal of Grey System2001，13，1： | 1 |
| 4 | Global Identification of Significantly Expressed Genes in Developing Endosperm of Rice by Expression Sequence Tags and cDNA Array Approaches显示文摘Rice endosperm plays a very important role in seedling germination and determines the qualities of rice grain. Although studies on specific gene categories in endosperm have been carried out, global view of gene expression at a transcription level in rice endosperm is still limited. To gain a better understanding of the global and tissue-specific gene expression profiles in rice endosperm, a cDNA library from rice endosperm of immature seeds was sequenced. A cDNA array was constructed based on the tentative unique transcripts derived from expression sequence tag (EST) assembling results and then hybridized with cDNAs from five different tissues or organs including endosperm, embryo, leaf, stem and root of rice. Significant redundancy was found for genes encoding prolamin, glutelin, allergen, and starch synthesis proteins, accounting for ~34% of the total ESTs obtained. The cDNA array revealed 87 significantly expressed genes in endosperm compared with the other four organs or tissues. These genes included 13 prolamin family proteins, 17 glutelin family proteins, 12 binding proteins, nine catalytic proteins and four ribosomal proteins, indicating a complicated biological processing in rice endosperm. In addition, Northern verification of 1,4-alpha-glucan branching enzyme detected two isoforms in rice endosperm, the larger one of which only existed in endosperm. | Qichao Tu Haitao Dong Haigen Yao Yongqi Fang Cheng'en Dai Hongmei Luo Jian Yao Dong Zhao Debao Li | 2008 | Journal of Integrative Plant Biology2008，50，9： | 0 |
| 5 | Multiple splicing types of OsRIX4,an RAD21 homolog in rice (Oryza sativa L.)显示文摘The present paper describes multiple splicing types of OsRIX4,an RAD21 homolog in rice. A type of alternative splicing (AS),distinctive from all five previously known splicing types,was identified in which interior sequences of a constitutive exon could be spliced. Translation of the transcript produced with this AS type was demonstrated at the protein level. Expression of multiple transcripts was organ specific. The expression abundance of transcripts,OsRIX4-4 and OsRIX4-5,was positively correlated with fertility in rice. The splicing type identified in the present study provided the means to further un-derstand and define different mRNA splicing types in plants and suggested that post-transcription processing of mRNA and its regulation mechanism are complex. | DONG HaiTaoi GUO XiaoQin PEI YanXi DAI ChengEn FANG YongQi TU QiChao ZHUANG JieYun ZHAO Dong ZHENG KangLe LI DeBao | 2007 | Chinese Science Bulletin2007，52，11： | 0 |
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