| 1 | Rab20 is critical for bacterial lipoprotein tolerization-enhanced bactericidal activity in macrophages during bacterial infection显示文摘Bacterial cell wall component-induced tolerance represents an important protective mechanism during microbial infection.Tolerance induced by the TLR2 agonist bacterial lipoprotein(BLP)has been shown to attenuate the inflammatory response,and simultaneously to augment antimicrobial function,thereby conferring its protection against microbial sepsis.However,the underlying mechanism by which BLP tolerance augments bactericidal activity has not been fully elucidated.Here,we reported that the induction of BLP tolerance in murine macrophages upregulated the expression of Rab20,a membrane trafficking regulator,at both the mRNA and protein levels upon bacterial infection.The knockdown of Rab20 with Rab20 specific siRNA(siRab20)did not affect the phagocytosis of Escherichia coli(E.coli),but substantially impaired the intracellular killing of the ingested E.coli in BLP-tolerized macrophages.Furthermore,Rab20 was associated with GFP-E.coli containing phagosomes,and BLP tolerization resulted in the enhanced maturation of GFP-E.coli-containing phagosomes associated with Rab20 and strong lysosomal acidification.The knockdown of Rab20 substantially diminished lysosome acidification and disturbed the fusion of GFP-E.coli containing phagosomes with lysosomes in BLP-tolerized macrophages.These results demonstrate that Rab20 plays a critical role in BLP tolerization-induced augmentation of bactericidal activity via promoting phagosome maturation and the fusion of bacteria containing phagosomes with lysosomes. | Shuqi Zhao Dalin Xi Junwei Cai Wenting Chen Jing Xiang Na Peng Juan Wang Yong Jiang Zhuzhong Mei Jinghua Liu | 2020 | Science China(Life Sciences)2020，63，3： | 2 |
| 2 | A Fast-Fractional Flow Reserve Simulation Method in A Patient with Coronary Stenosis Based on Resistance Boundary Conditions显示文摘Fractional flow reserve(FFR)is the gold standard to identify individual stenosis causing myocardial ischemia in catheter laboratory.The purpose of this study is to present a fast simulation method to estimate FFR value of a coronary artery,which can evaluate the performance of vascular stenosis,based on resistance boundary conditions.A patient-specific 3-dimensional(3D)model of the left coronary system with intermediate diameter stenosis was reconstructed based on the CTA images.The resistance boundary conditions used to simulate the coronary microcirculation were computed based on anatomical reconstruction of coronary 3D model.This study was performed by coupling the 3D coronary tree model with the lumped parameter model(0D model).The flow rate and pressure of coronary tree were calculated in twenty minutes.In addition,the effect of inlet pressure and myocardial mass on FFRss values has been investigated.The results showed that the effect of myocardial mass was greater than the effect of inlet pressure on FFRss.This FFRss simulation method can quickly and accurately assess the influence of coronary stenosis in aid clinical diagnosis. | Wenxin Wang Dalin Tang Boyan Mao Bao Li Xi Zhao Jian Liu Youjun Liu | 2018 | Computer Modeling in Engineering & Sciences2018，，8： | 1 |
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