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| 1 | Molecular cloning and functional analysis of the FLOWERING LOCUS T(FT) homolog Gh FT1 from Gossypium hirsutum显示文摘FLOWERING LOCUS T(FT) encodes a member of the phosphatidylethanolamine-binding protein(PEBP) family that functions as the mobile floral signal, playing an important role in regulating the floral transition in angiosperms. We isolated an FT-homolog(Gh FT1) from Gossypium hirsutum L. cultivar,Xinluzao 33 Gh FT1 was predominantly expressed in stamens and sepals, and had a relatively higher expression level during the initiation stage of fiber development. Gh FT1 m RNA displayed diurnal oscillations in both long-day and short-day condition,suggesting that the expression of this gene may be under the control of the circadian clock. Subcellular analysis revealed that Gh FT1 protein located in the cytoplasm and nucleus. Ectopic expression of Gh FT1 in transgenic arabidopsis plants resulted in early flowering compared with wild-type plants. In addition,ectopic expression of Gh FT1 in arabidopsis ft-10 mutants partially rescued the extremely late flowering phenotype. Finally, several Researchflowering related genes functioning downstream of At FT were highly upregulated in the 35S::Gh FT1 transgenic arabidopsis plants. In summary, Gh FT1 is an FT-homologous gene in cotton that regulates flower transition similar to its orthologs in other plant species and thus it may be a candidate target for promoting early maturation in cotton breeding. | Danli Guo Chao Li Rui Dong Xiaobo Li Xiangwen Xiao Xianzhong Huang | 2015 | Journal of Integrative Plant Biology2015，57，6： | 14 |
| 2 | Effects of protein in wheat flour on retrogradation of wheat starch 显示文摘 | LIAN Xijun GUO Junjie WANG Danli | 2014 | Journal of Food Science2014，79，8： | 1 |
| 3 | Expression and localization of vitellogenin genes(VTG)and receptor(VGR)in the gonad development of silver pomfret Pampus argenteus显示文摘Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous vitellogenin,which enters oocyte through endocytosis mediated by its receptor(VGR).We investigated the expression and localization of VTG and VGR during gonad development of Pampus argenteus.The qPCR results show that vtgs were not expressed in male fish,but in the ovary and liver of female fish;the expression levels went up at first and then down.The expression levels of vgr in the testis were low and only 1%-3%of that in ovary.ELISA results show that during the ovarian development of P.argenteus,VTG in liver,serum,and ovary all showed a trend from increasing to decreasing.However,VTG in liver peaked in StageⅣ,and in serum and ovary peaked in Stage V,reflecting changes in the characteristics of VTG in the liver(synthesis),blood(transport),and ovaries(accumulation).During gonad development,VGR in the ovaries first increased and then decreased,reaching a peak in Stage V,in contrast to vgr mRNA expression.The VGR content in the testis was extremely low and stable,consistent with vgr mRNA.Immunohistochemistry results show that the location and intensity of VTG and VGR positive signals were synchronized with the changes of their protein content,which revealed that VTG was mainly synthesized in the liver cytoplasm,secreted into the blood,and transported to ovary in StageⅢ.VGR is highly expressed in oocytes in StageⅡ.In StageⅢ,a large amount of VTG reaches the ovary,when VGR begins to translate and is subsequently transported to the plasma membrane of the oocyte.Therefore,the positive signal of VGR was stronger near the plasma membrane of oocytes in StagesⅠandⅡ.By using qPCR,ELISA,and immunohistochemistry,the synthesis,transport,and accumulation of vitellogenin were elucidated and the mechanism of its endocytosis on egg membrane mediated by VTG during the development of P.argenteus was revealed preliminarily. | Zitao XIONG Jiazhe YANG Shun ZHANG Yajun WANG Shanliang XU Chunyang GUO Danli WANG | 2023 | Journal of Oceanology and Limnology2023，41，4： | 0 |
| 4 | Tbx 4 and Tbx 5 gene expression associated with appendage development and its relationship with the absence of the pelvic fin in Pampus argenteus显示文摘The Tbx family is first known through the study of their functions in the body and limbs,and its members Tbx4 and Tbx5 genes are important factors in determining the characteristics of the appendages.Pampus argenteus is one of the important economical marine fishes widely distributed in offshore areas.Therefore,it is necessary to study the role of Tbx family genes in the deletion of pelvic fin in P.argenteus.In this study,we cloned Tbx4 and Tbx5 cDNA sequence of P.argenteus(GenBank:MH709128 and MH712458).The Western blot and real time PCR were used to detect the expressions of Tbx4 and Tbx5 in different developmental stages and tissues of P.argenteus.In addition,whole-mount in-situ hybridization was used to study the localization of Tbx4 and Tbx5 genes in different developmental stages of P.argenteus.Results show that the translation of Tbx4 mRNA was inhibited during the critical period of pelvic fin development.Among different tissues,Tbx4 protein levels were the lowest in the abdominal epithelium,and even lower than that in the pectoral fin,suggesting that the protein expression was also inhibited in the abdominal epithelium of adult P.argenteus.Therefore,the results indicated that upstream genes regulation led to the key stage-specific and low expression of Tbx4 during pelvic fin development and in the abdominal epithelium. | Shun ZHANG Xiaojing ZHU Lingzhu HU Kai LIAO Shanliang XU Danli WANG Chunyang GUO | 2024 | Journal of Oceanology and Limnology2024，42，2： | 0 |
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