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| 1 | CFD simulation on the generation of turbidites in deepwater areas: a case study of turbidity current processes in Qiongdongnan Basin, northern South China Sea显示文摘Turbidity currents represent a major agent for sediment transport in lakes, seas and oceans. In particular, they formulate the most significant clastic accumulations in the deep sea, which become many of the world's most important hydrocarbon reservoirs. Several boreholes in the Qiongdongnan Basin, the northwestern South China Sea, have recently revealed turbidity current deposits as significant hydrocarbon reservoirs. However, there are some arguments for the potential provenances. To solve this problem, it is essential to delineate their sedimentary processes as well as to evaluate their qualities as reservoir. Numerical simulations have been developed rapidly over the last several years, offering insights into turbidity current behaviors, as geologically significant turbidity currents are difficult to directly investigate due to their large scale and often destructive nature. Combined with the interpretation of the turbidity system based on highresolution 3D seismic data, the paleotophography is acquired via a back-stripping seismic profile integrated with a borehole, i.e., Well A, in the western Qiongdongnan Basin; then a numerical model is built on the basis of this back-stripped profile. After defining the various turbidity current initial boundary conditions, including grain size, velocity and sediment concentration, the structures and behaviors of turbidity currents are investigated via numerical simulation software ANSYS FLUENT. Finally, the simulated turbidity deposits are compared with the interpreted sedimentary bodies based on 3D seismic data and the potential provenances of the revealed turbidites by Well A are discussed in details. The simulation results indicate that a sedimentary body develops far away from its source with an average grain size of 0.1 mm, i.e., sand-size sediment. Taking into account the location and orientation of the simulated seismic line, the consistence between normal forward simulation results and the revealed cores in Well A indicates that the turbidites should have been transported from Vietnam instead of Hainan Island. This interpretation has also been verified by the planar maps of sedimentary systems based on integration of boreholes and seismic data. The identification of the turbidity provenance will benefit the evaluation of extensively distributed submarine fans for hydrocarbon exploration in the deepwater areas. | JIANG Tao ZHANG Yingzhao TANG Sulin ZHANG Daojun ZUO Qianmei LIN Weiren WANG Yahui SUN Hui WANG Bo | 2014 | Acta Oceanologica Sinica2014，33，12： | 3 |
| 2 | Poly(α-L-lysine)-based nanomaterials for versatile biomedical applications:Current advances and perspectives显示文摘Poly(α-L-lysine)(PLL)is a class of water-soluble,cationic biopolymer composed ofα-L-lysine structural units.The previous decade witnessed tremendous progress in the synthesis and biomedical applications of PLL and its composites.PLL-based polymers and copolymers,till date,have been extensively explored in the contexts such as antibacterial agents,gene/drug/protein delivery systems,bio-sensing,bio-imaging,and tissue engineering.This review aims to summarize the recent advances in PLL-based nanomaterials in these biomedical fields over the last decade.The review first describes the synthesis of PLL and its derivatives,followed by the main text of their recent biomedical applications and translational studies.Finally,the challenges and perspectives of PLL-based nanomaterials in biomedical fields are addressed. | Maochao Zheng Miao Pan Wancong Zhang Huanchang Lin Shenlang Wu Chao Lu Shijie Tang Daojun Liu Jianfeng Cai | 2021 | Bioactive Materials2021，6，7： | 2 |
| 3 | Molecular Characterization, Tissue Distribution and Localization of Larimichthys crocea Kif3a and Kif3b and Expression Analysis of Their Genes During Spermiogenesis显示文摘KIF3A and KIF3B are two N-terminal motor proteins belonging to the kinesin-II superfamily that play essential roles in spermiogenesis.To understand the roles played by KIF3 A/3B during spermatogenesis of large yellow croaker Larimichthys crocea,we studied the testis characteristics at different developmental stages of L.crocea,and determined the spatiotemporal expression patterns of kif3a and kif3b during spermiogenesis.Quantitative real-time PCR(qR T-PCR)showed that the overall trends of kif3 a/3 b m RNA abundance during testis development are similar.From stage Ⅱ to stage V,kif3a/3b m RNA abundances first increased and then fell after reaching a peak at stage IV.Interestingly,the m RNA abundances of both genes at stage V were higher than those at stages Ⅱ and Ⅲ.In addition,it is worth of noting that kif3 b m RNA abundance was higher than that of kif3a at all stages.Fluorescence in situ hybridization results revealed that kif3a/3b m RNA abundance dynamics were consistent with the migration of mitochondria,the deformation of nucleus,and the formation of tail.The m RNA hybridization signals of both genes first appeared either around the nuclear periphery or on the side of the nuclei,then appeared at one side of nuclei,and finally were mainly on the tail during spermiogenesis.Our findings contributed to better understanding the molecular mechanisms of spermiogenesis in fish;and suggested that KIF3A and KIF3B may participate in the intracellular transport of mitochondria,nuclear deformation,and the formation of tail during the spermiogenesis in L.crocea. | MU Danli DU Chen FU Suyan WANG Jingqian HOU Congcong TANG Daojun ZHU Junquan | 2019 | Journal of Ocean University of China2019，18，6： | 0 |
| 4 | Characterization,expression dynamics,and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta显示文摘Mitochondria undergo morphological changes during spermatogenesis in some animals.The mechanism and role of mitochondrial morphology regulation,however,remain somewhat unclear.In this study,we analyzed the molecular characteristics,expression dynamics and subcellular localization of optic atrophy protein 1(OPA1),a mitochondrial fusion and cristae maintenance-related protein,to reveal the possible regulatory mechanisms underlying mitochondrial morphology in Phascolosoma esculenta spermiogenesis.The full-length cDNA of the P.esculenta opa1 gene(Pe-opa1)is 3743 bp in length and encodes 975 amino acids.The Pe-OPA1 protein is highly conservative and includes a transmembrane domain,a GTPase domain,two helical bundle domains,and a lipid-interacting stalk.Gene and protein expression was higher in the coelomic fluid(a site of spermatid development)of male P.esculenta and increased first and then decreased from March to December.Moreover,their expression during the breeding stage was significantly higher than during the non-breeding stage,suggesting that Pe-OPA1 is involved in P.esculenta reproduction.The Pe-OPA1 protein was more abundant in components consisting of many spermatids than in components without,indicating that Pe-OPA1 mainly plays a role in the spermatid in coelomic fluid.Moreover,Pe-OPA1 was mainly detected in the spermatid mitochondria.Immunofluorescence experiments showed that the Pe-OPA1 are constitutively expressed and co-localized with mitochondria during spermiogenesis,suggesting its involvement in P.esculenta spermiogenesis.These results provide evidence for Pe-OPA1's involvement in the regulation of mitochondrial morphology during spermiogenesis. | Xinming GAO Binbin FENG Chen DU Congcong HOU Shan JIN Daojun TANG Junquan ZHU | 2024 | Journal of Oceanology and Limnology2024，42，1： | 0 |
| 5 | Prohibitin Protein Expression During Spermatogenesis in the Large Yellow Croaker, Larimichthys crocea显示文摘Mitochondria are important for animals’fertility,and their morphologies and functions during spermatogenesis are un-der investigation.However,the molecular mechanism that regulates the mitochondrial dynamic during spermatogenesis is still un-known.In this study,the cytological features of spermatogenesis were investigated in Larimichthys crocea.In addition,the structure and function of prohibitin(PHB),which is associated with mitochondrial structure and dynamic,was also investigated.The full-length cDNA and protein(Lc-PHB)from the L.crocea phb gene(Lc-phb)contained 1625 base pairs and 271 amino acids,respec-tively.Lc-PHB had a conserved primary structure that resulted in a transmembrane,SPFH(the analogous region of proteins stomat-ins,prohibitins,flotillins and HflK/C),and coiled-coil domains.It was detected at high levels in the muscle,liver,and heart,and at intermediate levels in the testis,gill,and brain.Lc-phb mRNA expression was detected in spermatogenic cells by fluorescence in situ hybridization.An immunofluorescence assay revealed that PHB protein was localized in the mitochondria during spermatogenesis.Specifically,PHB expression was detected in the perinuclear cytoplasm of spermatogonia,spermatocytes,and spermatids in the early developmental stage,and mainly localized on one side of the nuclei in the cytoplasm of spermatids in a middle developmental stage,and finally on the sperm midpiece.Western blotting showed that PHB was located in the extracted mitochondria protein fraction but not in the cytoplasm protein fraction of testes.Conclusively,these results indicated that PHB was expressed in the mitochondria dur-ing spermatogenesis.In addition,the study explained the mitochondrial dynamic during fish spermatogenesis and proposed a possi-ble relationship among PHB,spermatogenesis,and male fertility. | LIN Chenwen GAO Xinming NI Jie ZHANG Shengshuo LIU Cheng LUO Shengyu DU Chen ZHENG Xuebin HOU Congcong TANG Daojun ZHANG Chundan ZHU Junquan | 2022 | Journal of Ocean University of China2022，21，5： | 0 |
| 6 | Physiological and histological responses of Phascolosoma esculenta (Sipuncula: Phascolosomatidea) to acute heat stress显示文摘Temperature is an important factor affecting the growth,development,and survival of organisms.The effects of temperature stress on aquatic organisms have received increasing attention,as these organisms are mostly poikilotherms and their body temperature are directly corresponding changes with ambient temperature,resulting in they are easily exposed in temperature stress.However,little is known about the effects of high temperature on Sipuncula.In this study,we investigated the effects of acute heat stress on malondialdehyde(MDA)concentration,the activities of antioxidant(superoxide dismutase and glutathione peroxidase)and immunity-related(acidic and alkaline phosphatase)enzymes,heat shock protein 70(hsp70)and hsp90 gene expression,and the histological structure of the sipunculid Phascolosoma esculenta.Within the coelom fl uid,the MDA concentration and all detected antioxidant enzyme activities increased during high temperature stress;signifi cant increases were also observed here and in the intestine in the hsp70 and hsp90 mRNA expression levels.These results indicated that acute heat stress caused oxidative stress;antioxidants and heat shock proteins probably act to protect P.esculenta against oxidative damage,constituting part of its physiological mechanism for adaptation to high temperatures.In addition,the increased activity of the acidic and alkaline phosphatases indicated eff ects on its nonspecifi c immune system.Furthermore,damaged tissue structures were observed in the body wall,retractor muscle,intestine,and nephridium after 96 h of 40-℃ stress.The damaged cells of these tissues showed obvious condensed chromatin around the nuclear membrane.This histological damage suggests that heat stress could affect movement,food absorption,digestion,and excretion in P.esculenta.These results elucidate the effects of temperature stress on P.esculenta and its physiological response mechanisms and provide practical indicators for assessing heat stress status and determining suitable culture temperatures for P.esculenta. | Xinming GAO Haiyan YANG Daojun TANG Chen DU Shan JIN Congcong HOU Chundan ZHANG Junquan ZHU Jianping WANG | 2022 | Journal of Oceanology and Limnology2022，40，2： | 0 |
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