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| 1 | Heparin improves organ microcirculatory disturbances in caerulein-induced acute pancreatitis in rats显示文摘AIM: Microcirculatory disturbances are important early pathophysiological events in various organs during acute pancreatitis. The aim of the study was to evaluate changes in rnicroperfusion of the pancreas, liver, kidney, stomach,colon, skeletal muscle, and to investigate the influence of heparin on the organ rnicrocirculation in caerulein-induced experimental acute pancreatitis.METHODS: Acute pancreatitis was induced by 4 intraperitoneal injections of caerulein (Cn) (15 μg/kg). The organ microcirculation was measured by laser Doppler flowrnetry. Serum interleukin 6 and hernatocrit levels were analysed.RESULTS: Acute pancreatitis resulted in a significant drop of microperfusion in all examined organs. Heparin administration (2×2.5 mg/kg) improved the rnicrocirculation in pancreas (36.9±4% vs75.9±10%), liver (56.6±6% vs 75.2±16%), kidney (45.1±6% vs79.3±5%), stomach (65.2±8% vs78.1±19%), colon (69.8±6% vs 102.5±19%),and skeletal muscle (59.2±6% vs 77.9±13%). Heparin treatment lowered IL-6 (359.0±66 U/mL vs 288.5±58 U/mL) and hematocrit level (53±4% vs 46±3%).CONCLUSION: Hepadn administration has a positive influence on organ microcirculatory disturbances accompanying experimental Cn-induced acute pancreatitis. | Marek Dobosz Lucjanna Mionskowska Stanislaw Ha■ Sebastian Dobrowolski Dariusz Dymecki Zdzislaw Wajda | 2004 | World Journal of Gastroenterology2004，10，17： | 34 |
| 2 | Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues显示文摘BACKGROUND Mesenchymal stromal/stem cells (MSCs) constitute a promising tool in regenerative medicine and can be isolated from different human tissues. However, their biological properties are still not fully characterized. Whereas MSCs from different tissue exhibit many common characteristics, their biological activity and some markers are different and depend on their tissue of origin. Understanding the factors that underlie MSC biology should constitute important points for consideration for researchers interested in clinical MSC application. AIM To characterize the biological activity of MSCs during longterm culture isolated from: bone marrow (BM-MSCs), adipose tissue (AT-MSCs), skeletal muscles (SMMSCs), and skin (SK-MSCs). METHODS MSCs were isolated from the tissues, cultured for 10 passages, and assessed for: phenotype with immunofluorescence and flow cytometry, multipotency with differentiation capacity for osteo-, chondro-, and adipogenesis, stemness markers with qPCR for mRNA for Sox2 and Oct4, and genetic stability for p53 and c-Myc;27 bioactive factors were screened using the multiplex ELISA array, and spontaneous fusion involving a co-culture of SM-MSCs with BM-MSCs or AT-MSCs stained with PKH26 (red) or PKH67 (green) was performed. RESULTS All MSCs showed the basic MSC phenotype;however, their expression decreased during the follow-up period, as confirmed by fluorescence intensity. The examined MSCs express CD146 marker associated with proangiogenic properties;however their expression decreased in AT-MSCs and SM-MSCs, but was maintained in BM-MSCs. In contrast, in SK-MSCs CD146 expression increased in late passages. All MSCs, except BM-MSCs, expressed PW1, a marker associated with differentiation capacity and apoptosis. BM-MSCs and AT-MSCs expressed stemness markers Sox2 and Oct4 in long-term culture. All MSCs showed a stable p53 and c-Myc expression. BM-MSCs and AT-MSCs maintained their differentiation capacity during the follow-up period. In contrast, SK-MSCs and SM-MSCs had a limited ability to differentiate into adipocytes. BM-MSCs and AT-MSCs revealed similarities in phenotype maintenance, capacity for multilineage differentiation, and secretion of bioactive factors. Because AT-MSCs fused with SM-MSCs as effectively as BM-MSCs, AT-MSCs may constitute an alternative source for BM-MSCs. CONCLUSION Long-term culture affects the biological activity of MSCs obtained from various tissues. The source of MSCs and number of passages are important considerations in regenerative medicine. | Urszula Kozlowska Agnieszka Krawczenko Katarzyna Futoma Tomasz Jurek Marta Rorat Dariusz Patrzalek Aleksandra Klimczak | 2019 | World Journal of Stem Cells2019，11，6： | 10 |
| 3 | Preoperative high level of D-dimers predicts unresectability of pancreatic head cancer显示文摘AIM: To assess the value of D-dimer level in determining resectability of pancreatic cancer. METHODS: Preoperative prediction of pancreatic head cancer resectability remains inaccurate. The use of hemostatic factors may be of potential help, since D-dimers correlate with tumor stage. Single center clinical trial study comprised patients with potentially resectable pancreatic head tumor and without detectable venous thrombosis(n = 64). Resectability was defined as no evidence of nodal involvement, distant spread and no invasion of mesenteric vessels. Final decision of resectability was confirmed intraoperatively. Experienced pancreatic surgeon performed all surgeries. Following the dissection of hepatoduodenal ligament, samples of portal blood and bile were taken. Peripheral blood via central line and urine via Foley catheter were sampled. D-dimer levels were further measured.RESULTS: At laparotomy only 29(45.3%) tumors were found to be resectable. Our analysis showed higher by 57.5%(P < 0.001) mean D-dimer values in peripheral and 43.7%(P = 0.035) in portal blood of patients with unresectable pancreatic cancer. Significant differences were not observed when analyzing D-dimer levels in bile and urine. Peripheral D-dimer level correlated with pancreatic cancer resectability. When cut-off D-dimer value of 570.6 μg/L was used, the sensitivity for assessment of tumor unresectability was 82.8%. Furthermore, D-dimer level in peripheral blood of metastatic disease(n = 15) was significantly higher when compared to locally advanced(n = 20) pancreatic cancer(2470 vs 1168, P = 0.029). The area under ROC curve for this subgroup of patients was 0.87; for determination of unresectable disease when threshold of 769.8 μg/L was used, sensitivity and specificity was 86.6% and 80%, respectively.CONCLUSION: Patients with resectable pancreatic head cancer based on preoperative imaging studies and high D-dimer level may be considered unresectable due to occult hepatic metastases. These patients may benefit from diagnostic laparoscopy to avoid exploratory laparotomy. | Adam Durczynski Anna Kumor Piotr Hogendorf Dariusz Szymanski Piotr Grzelak Janusz Strzelczyk | 2014 | World Journal of Gastroenterology2014，20，36： | 9 |
| 4 | 应用有限元法(FEM)验证在直齿轮和斜齿轮内采用ISO和AGMA标准的齿根强度的比较分析显示文摘在工程界现在普遍的趋势是要求重新研究各不同的标准以确是其在工业内最好的实践。本文介绍采用在ISO和AGMA标准对齿根强度估算方法的比较分析,并用开发的有限元和模型仿真进行证实。本分析(1)介绍了采用齿条和齿轮刀具制造的广大范围的直齿和斜齿轮,而(2)是齿轮主要几何学(齿轮设计),制造(齿条和齿轮刀具)和性能参数的不同组合,以及对每个齿轮进行齿根强度分析的有限元方法(FEM)。FEM的结果与用ISO和AGMA标准计算的相比较。对不同的设计、制造和性能参数组合的比较分析用图解说明和简要讨论。该结果将许可在现代工程实际中应用标准所存在的限制有一个很好的了解,并为改进和统一标准提供一个基础。 | Andrzej kawalec Jerzy Wiktor Dariusz Ceglarek | 2007 | 传动技术2007，21，2： | 8 |
| 5 | Laser-induced white-light emission from graphene ceramics–opening a band gap in graphene显示文摘Recent theoretical and experimental studies have indicated the existence of a new stable phase of carbon with mixed sp^(2) and sp^(3) hybridized bonds—diaphite.Such a two-layered structure with sp^(2)/sp^(3) bonds may be observed after the photostimulation of highly oriented pyrolytic graphene with femtosecond laser pulses.This hidden multistability of graphene may be used to create a semiconducting phase immersed in the semimetallic continuum,resulting in bandgap opening.We demonstrate that bandgap opening and light emission from graphene is possible using continuous-wave laser beams with wavelengths from the visible(405 nm)to the near-infrared range(975 nm).We demonstrate that without the application of cooling,the effective temperature of the emitting sample remains lower than 900 K,which is far below the value predicted by the theory of black-body radiation.Moreover,light emission from a graphene sample may be observed at temperatures as low as 10 K. | Wieslaw Strek Bartlomiej Cichy Lukasz Radosinski Pawel Gluchowski Lukasz Marciniak Mikolaj Lukaszewicz Dariusz Hreniak | 2015 | Light(Science & Applications)2015，4，1： | 6 |
| 6 | 帕博利珠单抗联合以铂类为基础的化疗治疗非小细胞肺癌合并稳定性脑转移患者的结局:KEYNOTE-021、-189和-407研究的汇总分析显示文摘背景与目的此项探索性分析回顾评价了晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)患者的结局,旨在确定基线时合并脑转移是否会影响一线应用帕博利珠单抗联合化疗(pembrolizumab plus chemotherapy,PC)比对单用化疗的疗效。患者和方法对KEYNOTE-021队列G(非鳞癌)、KEYNOTE-189(非鳞癌)和KEYNOTE-407(鳞癌)三项研究晚期NSCLC患者的数据进行汇总分析。患者接受含铂两药化疗和/或35个周期帕博利珠单抗治疗(每3周200 mg)。所有研究均纳入了经治或初治的稳定性脑转移患者(KEYNOTE-189与KEYNOTE-407研究纳入初治脑转移患者)。经治的脑转移患者已处于病情稳定的状态≥2周(KEYNOTE-021队列G患者≥4周),无新发或脑转移病灶扩大的证据且入组前至少3天以上未使用激素。初治的无症状脑转移患者需定期接受脑部影像学检查。结果共纳入1298例患者,其中171例有基线脑转移,1127例无脑转移。两组的中位随访时间(范围)在数据截止时分别为10.9(0.1-35.1)个月和11.0(0.1-34.9)个月。合并脑转移和无脑转移患者的总生存期[0.48(95%CI:0.32-0.70)和0.63(95%CI:0.53-0.75)]和无进展生存期[0.44(95%CI:0.31-0.62)和0.55(95%CI:0.48-0.63)]的风险比(PC/化疗)相似。合并脑转移的患者中,PC组和单用化疗组患者的中位总生存期分别为18.8个月和7.6个月,中位无进展生存期分别为6.9个月和4.1个月。无论是否合并脑转移,PC组患者的客观缓解率都高于单用化疗组,且缓解持续时间有显著延长。合并脑转移的患者中,PC组与单用化疗组的治疗相关不良事件发生率分别为88.2%和82.8%;而无脑转移的患者中,两组治疗相关不良事件的发生率分别为94.5%和90.6%。结论无论是否合并脑转移,与单用化疗相比,帕博利珠单抗联合以铂类为基础的组织学特异性化疗可改善所有PD-L1亚组的临床结局,其中包括PD-L1肿瘤比例评分<1%的患者,并且在晚期NSCLC患者中安全性良好。该方案是晚期NSCLC初治患者的标准方案,并可用于合并稳定性脑转移的患者。 | 周清(翻译校对) Steven F Powell Delvys Rodríguez-Abreu Corey J Langer Ali Tafreshi Luis Paz-Ares Hans-Georg Kopp Jeronimo Rodríguez-Cid Dariusz M Kowalski Ying Cheng Takayasu Kurata Mark M Awad Jinaxin Lin Bin Zhao M Catherine Pietanza Bilal Piperdi Marina C Garassino | 2022 | 中国肺癌杂志2022，25，1： | 6 |
| 7 | Ultrastructure of oval cells in children with chronic hepatitis B,with special emphasis on the stage of liver fibrosis:The first pediatric study显示文摘AIM:To investigate the ultrastructure of oval cells in children with chronic hepatitis B,with special emphasis on their location in areas of collagen fibroplasia. METHODS:Morphological investigations were conducted on biopsy material obtained from 40 children,aged 3-16 years with chronic hepatitis B. The stage of fibrosis was assessed histologically using the arbitrary semiquantitative numerical scoring system proposed by Ishak et al. The material for ultrastructural investigation was fixed in glutaraldehyde and paraformaldehyde and processed for transmission-electron microscopic analysis. RESULTS:Ultrastructural examination of biopsy specimens obtained from children with chronic hepatitis B showed the presence of two types of oval cells,the hepatic progenitor cells and intermediate hepatic-like cells. These cells were present in the parenchyma and were seen most commonly in areas of intense periportal fibrosis (at least stage 2 according to Ishak et al) and in the vicinity of the limiting plate of the lobule. The activated nonparenchymal hepatic cells,i.e. transformed hepatic stellate cells and Kupffer cells were seen in close proximity to the intermediate hepatic-like cells. CONCLUSION:We found a distinct relationship between the prevalence of oval cells (hepatic progenitor cells and intermediate hepatocyte-like cells) and fibrosis stage in pediatric patients with chronic hepatitis B. | Maria Elzbieta Sobaniec-Lotowska Joanna Maria Lotowska Dariusz Marek Lebensztejn | 2007 | World Journal of Gastroenterology2007，13，21： | 6 |
| 8 | Discrete-time dynamic graphical games:model-free reinforcement learning solution显示文摘This paper introduces a model-free reinforcement learning technique that is used to solve a class of dynamic games known as dynamic graphical games. The graphical game results from multi-agent dynamical systems, where pinning control is used to make all the agents synchronize to the state of a command generator or a leader agent. Novel coupled Bellman equations and Hamiltonian functions are developed for the dynamic graphical games. The Hamiltonian mechanics are used to derive the necessary conditions for optimality. The solution for the dynamic graphical game is given in terms of the solution to a set of coupled Hamilton-Jacobi-Bellman equations developed herein. Nash equilibrium solution for the graphical game is given in terms of the solution to the underlying coupled Hamilton-Jacobi-Bellman equations. An online model-free policy iteration algorithm is developed to learn the Nash solution for the dynamic graphical game. This algorithm does not require any knowledge of the agents' dynamics. A proof of convergence for this multi-agent learning algorithm is given under mild assumption about the inter-connectivity properties of the graph. A gradient descent technique with critic network structures is used to implement the policy iteration algorithm to solve the graphical game online in real-time. | Mohammed I.ABOUHEAF Frank L.LEWIS Magdi S.MAHMOUD Dariusz G.MIKULSKI | 2015 | Control Theory and Technology2015，13，1： | 6 |
| 9 | Major complications following exenteration in cases of pelvic malignancy:A 10-year experience显示文摘瞄准:在妇产科、直肠的恶意的取出肚肠以后分析主要复杂并发症。方法:有妇产科的恶意的 22 个病人并且 6 与直肠的恶意经历了在 1996 和 2005 之间的盆腔脏器除去术(PE ) 。 PE 在71.4%盒子中为主要恶意被执行(在 13 的外阴癌症,在 5 直肠的癌症,在 1 的颈的癌症和 Bartholin 分别地是在 1 种情况中的腺癌症并且在28.6%盒子中的周期性的恶意(在 5 的颈的癌症,在 1 的卵巢的癌症,在 1 的子宫的肉瘤和在 1 种情况中的直肠的癌症分别地)。以后的 PE,全部的 PE 和前面的 PE 最经常被执行。结果:包含泌尿道感染或伤口裂开的起作用的领域里的主要复杂并发症发生在 12 个病人(42.9%) 。早复杂并发症包括了从骶的丛,成年呼吸窘迫症候群(ARDS ) ,血栓静脉炎,尖锐肾衰竭,膀胱机能障碍,输尿管损坏,重新操作和肺的栓子的巨大的流血。尿失禁作为迟了的复杂并发症在 2 个女人被观察。在 1 个病人,肾造口术由于有的复杂并发症与胃肠道连接了的广泛的肾盂积水膨出和 1 个病人在 1 个病人被执行。死亡率是 7% ,哪个内部起作用的死亡占了 3.5% 。主要复杂并发症经常在与周期性的恶意影响那些的先进主要外阴癌症发生了。结论:PE 比到有周期性的癌症的那些对有主要外阴、直肠的癌症的病人更有益。固有的复杂并发症的知识和 PE 的病态是必要的。 | Dariusz Wydra Janusz Emerich Sambor Sawicki Katarzyna Ciach Andrzej Marciniak | 2006 | World Journal of Gastroenterology2006，12，7： | 5 |
| 10 | 全球国际收支失衡:欧洲的观点显示文摘 | Alan Ahearne Klaus Regling In Jun Kim Dariusz Rosati 施建淮 | 2006 | 国际经济评论2006，，5： | 5 |
| 11 | Effect of interferon alpha and ribavirin treatment on serum levels of transforming growth factor-β1,vascular endothelial growth factor,and basic fibroblast growth factor in patients with chronic hepatitis C显示文摘AIM:To assess the role of transforming growth factor-β1(TGF-β1),vascular endothelial growth factor(VEGF)andbasic fibroblast growth factor(bFGF)in the pathogenesisof fibrosis associated with chronic hepatitis C(CHC)and to evaluate the influence of the antiviral therapyon above parameter levels depending on the treatmentresults(complete response or no response).METHODS:Study group included 100 patients withCHC,in whom fibrosis in liver specimens was assessed(Scheuer fibrosis score:1-4 points).Control groupincluded 30 subjects with antibodies anti-HCV presentand persistently normal ALT level,without fibrosis(Scheuer fibrosis score:0 points).Concentration ofstudied parameters was assayed in the serum byimmunoenzymatic method before and after the therapywith interferon alpha-2b and ribavirin.RESULTS:TGF-β1 levels were significantly higher in thestudy group compared to the control group(35.89 vs 32.37ng/mL;P=0.023).Such differences were not found in VEGFand bFGF levels.In patients showing complete response(negative HCV RNA and normal ALT level),significantincrease in VEGF(112.8 vs 315.03 pg/mL;P<0.05)andbFGF(2.51 vs 15.79 pg/mL;P=0.04)levels were found.Significant decrease in TGF-β1 level was observed bothin responders(37.44 vs 30.02 ng/mL;P=0.05),andin non-responders(38.22 vs 30.43 ng/mL;P=0.043).bFGF levels before the treatment were significantly lower(2.51 vs 5.94 pg/mL;P=0.04),and after the treatmentsignificantly higher(15.79 vs 4.35 pg/mL;P=0.01) in patients with complete response than in those with noresponse.CONCLUSION:Among the analyzed parametersTGF-β1 seems to play the most important role in thepathogenesis of fibrosis in CHC.Levels of this factor aresignificantly lower in subjects who do not have fibrosisdeveloped in them.Good therapeutic effect in CHCpatients is associated with significant changes in TGF-β1,VEGF,and bFGF levels,bFGF seems to have the highestusefulness in the prognosis of treatment efficacy. | Ewa Janczewska-Kazek Bogdan Marek Dariusz Kajdaniuk Halina BorgieI-Marek | 2006 | World Journal of Gastroenterology2006，12，6： | 5 |
| 12 | 综述前苏联关于地表沉陷的煤炭地下气化经验(英文)显示文摘1.相比于传统的煤矿开采方法,煤炭地下气化的适用范围更广,但同时也可能引起地下水污染和地表沉陷。2.前苏联在煤炭地下气化方面拥有一段很长的研究和使用历史,由于当时的国际环境,前苏联的研究相对比较封闭,所以对其研究成果的综述目前仍然很有价值。3.综述了不同煤层参数(如煤层深度、倾斜度、高度、宽度、含灰量和地质剖面等)和气化燃烧相关参数(如岩土材料导热性、热损耗、热冲击和体积变化等)与地表沉陷的相关性研究。 | Yury DERBIN James WALKER Dariusz WANATOWSKI Alec MARSHALL | 2015 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2015，16，10： | 5 |
| 13 | Assessment of sealed fire states by fire characteristic显示文摘The paper presented assessment of sealed fire states in underground coal mines by so-called 'fire characteristic', which graphically described tendencies of fire gas components-oxygen, nitrogen, carbon dioxide, carbon monoxide and hydrocarbons-in time. In order to mark gas components tendencies in time the authors applied the time series analysis. The case studied confirmed, that analysis of fire gas components tendencies in time and their correlation allow to elicit proper conclusions about fire state assessment. Assessment of fire states based on single value of fire indexes without considering their trends in time and correlation between trends of gas components would give wrong results The suggested method can appropriately indicate fire states in a sealed area. | 袁树杰 SZLAZAK Nikodem OBRACAJ Dariusz | 2006 | Journal of Coal Science & Engineering(China)2006，12，1： | 4 |
| 14 | The influence of potassium to mineral fertilizers on the maize health显示文摘Field experiments(2009–2011) were conducted at the Department of Agronomy at Poznań University of Life Sciences on the fields of the Research Institute in Swadzim. We evaluated the health of maize plants of two types, depending on the variations in mineral fertilization. The conducted research recorded the occurrence of pests such as oscinella frit(Oscinella frit L.) and the European corn borer(Pyrausta nubilalis Hbn.). Diseases recorded during the research included two pathoagenes: Fusarium(Fusarium ssp.) and corn smut(Ustilago maydis Corda). It was shown that the meteorological conditions during the maize vegetation had a significant influence on the occurrence of pests. Adding potassium to mineral fertilizers increased the maize resistance to Fusarium. Cultivation of 'stay-green' cultivar shall be considered as an element of in-tegrated maize protection. The occurrence of oscinella frit was correlated with the occurrence of Fusarium as well as the occurrence of the European corn borer for both examined cultivars. | Jan Bocianowski Piotr Szulc Anna Tratwal Kamila Nowosad Dariusz Piesik | 2016 | Journal of Integrative Agriculture2016，15，6： | 4 |
| 15 | Random checkerboard based homogenization for estimating effective thermal conductivity of fully saturated soils显示文摘This paper proposes homogenization scheme for estimating the effective thermal conductivity of fully saturated soils. This approach is based on the random checkerboard-like microstructure. Two modeling scales and two modeling approaches are distinguished and used, i.e. microscale and mesoscale and 1-step and 2-step homogenizations, respectively. The 2-step homogenization involves sequential averaging procedure, i.e. first, at microscale, a mineralogical composition of soil skeleton is considered and averaging process results in estimation of the skeleton effective thermal conductivity, and then, at mesoscale, a random spatial packing of solid skeleton and pores via random checkerboard microstructure is modeled and leads to evaluation of the soil overall thermal conductivity. The 1-step homogenization starts directly at the mesoscale and homogenization procedure yields evaluation of the overall soil thermal conductivity. At the mesoscale, the distinct nature of soil skeleton, as composed of soil separates,is considered and random variability of soil is modeled via enriched random checkerboard-like structure.Both approaches, i.e. 1-step and 2-step homogenizations, interrelate mineralogical composition with the soil texture characterized by the volume fractions of soil separates, i.e. sand, silt and clay. The probability density functions(PDFs) of thermal conductivity are assumed for each of the separates. The soil texture PDF of thermal conductivity is derived taking into consideration the aforementioned functions. Whenever the random checkerboard-like structure is used in averaging process, the Monte Carlo procedure is applied for estimation of homogenized thermal conductivity. Finally, the proposed methodology is tested against the laboratory data from our measurements as well as those available from literature. | Dariusz Lydzba Adrian Rózanski Magdalena Rajczakowska Damian Stefaniuk | 2017 | Journal of Rock Mechanics and Geotechnical Engineering2017，9，1： | 4 |
| 16 | Pediatric non-alcoholic steatohepatitis:The first report on the ultrastructure of hepatocyte mitochondria显示文摘AIM:To investigate the ultrastructure of abnormal hepatocyte mitochondria,including their cellular and hepatic zonal distribution,in bioptates in pediatric nonalcoholic steatohepatitis(NASH).METHODS:Ultrastructural investigations were conducted on biopsy liver specimens obtained from 10children(6 boys and 4 girls)aged 2-14 years with previously clinicopathologically diagnosed NASH.The disease was diagnosed if liver biopsy revealed steatosis,inflammation,ballooned hepatocytes,Mallory hyaline,or focal necrosis,varying degrees of fibrosis in the absence of clinical,serological,or histological findings of infectious liver diseases,autoimmune hepatitis,metabolic liver diseases,or celiac disease.For ultrastructural analysis,fresh small liver blocks(1 mm3 volume)were fixed in a solution containing 2%paraformaldehyde and 2.5%glutaraldehyde in 0.1 mol/L cacodylate buffer.The specimens were postfixed in osmium tetroxide,subsequently dehydrated through a graded series of ethanols and propylene oxide,and embedded in Epon812.The material was sectioned on a Reichert ultramicrotome to obtain semithin sections,which were stained with methylene blue in sodium borate.Ultrathin sections were contrasted with uranyl acetate and lead citrate,and examined using an Opton EM 900 transmission electron microscope.RESULTS:Ultrastructural analysis of bioptates obtained from children with non-alcoholic steatohepatitis revealed characteristic repetitive mitochondrial abnormalities within hepatocytes;mainly mitochondrial polymorphisms such as megamitochondria,loss of mitochondrial cristae,and the presence of linear crystalline inclusions within the mitochondrial matrix of an increased electron density.The crystalline inclusions were particularly evident within megamitochondria(MMC),which seemed to be distributed randomly both within the hepatic parenchymal cell and the zones of hepatic lobule,without special variations in abundance.The inclusions appeared as bundles viewed longitudinally,or as an evenly spaced matrix in cross section,and frequently caused mitochondrial deformation.The average diameter of these linear structures was 10 nm and the average space between them 20 nm.Sometimes enlarged intramitochondrial granules were seen in their vicinity.Foamy cytoplasm of hepatocytes was found,resulting from the proliferation of smooth endoplasmic reticulum and glycogen accumulation.The perivascular space of Disse was frequently dilated,and contained transitional hepatic stellate cells,as well as mature and/or newly forming collagen fiber bundles.CONCLUSION:Marked ultrastructural abnormalities observed in hepatocyte mitochondria,especially their polymorphism in the form of MMC and loss of mitochondrial cristae,accompanied by foamy cytoplasm,clearly indicate a major role of these organelles in the morphogenesis of pediatric NASH.Our findings seem to prove the high effectiveness of electron microscopy in the diagnosis of the disease. | Joanna M Lotowska Maria E Sobaniec-Lotowska Sylwia B Bockowska Dariusz M Lebensztejn | 2014 | World Journal of Gastroenterology2014，20，15： | 3 |
| 17 | Histological outcome of chronic hepatitis B in children treated with interferon alpha显示文摘AIM: To evaluate the effect of interferon alpha (IFN-α)treatment on the liver histology in children with chronic hepatitis B and to evaluate the usefulness of various histological scoring systems of liver histology in this group of patients.METHODS: Fibrosis stage and inflammation grade were assessed according to Batts and Ludwig, Ishak et al., and METAVIR (only fibrosis stage) before and 12 mo after IFN-α treatment termination in 93 children aged 2-16years with chronic hepatitis B.RESULTS: None of the three numerical scoring systems for liverfibrosis showed statistically significant differences in liver fibrosis, while evolution of inflammatory activity revealed statistically significant improvement in the whole group of children with chronic hepatitis B treated with IFN-α and in responders. Significantly positive correlations were found between fibrosis stage and inflammation grade in the respective scoring systems.CONCLUSION: Treatment with IFN-α did not improve histological fibrosis but decreased inflammatory activity in children with chronic hepatitis B. The three semiquantitative scoring systems seem to be comparable in the estimation of the inflammation grade and fibrosis stage in this group of children. | Sobaniec-Lotowska Maria Elzbieta Lebensztejn Dariusz Marek | 2005 | World Journal of Gastroenterology2005，11，45： | 3 |
| 18 | Thermal properties of AlN polycrystals obtained by pulse plasma sintering method显示文摘Aluminum nitride(AlN)polycrystals were prepared by pulse plasma sintering(PPS)technique.The starting AlN powder mixtures were composed with 3.0 wt%,5.0 wt%and 10 wt%of yttrium oxide(Y_(2)O_(3)),respectively.Relative density of each polycrystal was measured by hydrostatic method and evaluated higher than 97%.X-ray diffraction(XRD)method was used for phase examination of the samples after heat treatment.Microstructure examination supported by computer-aided analysis was performed by scanning electron microscopy(SEM)and energy dispersive spectrometry(EDS).The results were correlated with thermal conductivity of the samples carried out by laser pulse method(LFA).The influence of the rapid sintering technique and yttrium oxide additive content on the thermal conductivity and microstructure appearance of AlN polycrystals was clearly shown. | PawełJ.RUTKOWSKI Dariusz KATA | 2013 | Journal of Advanced Ceramics2013，2，2： | 3 |
| 19 | GDF-15 Level Correlates with CMKLR1 and VEGF-A in Tumor-free Margin in Colorectal Cancer显示文摘Colorectal cancer(CRC)is the third most frequently diagnosed cancer worldwide,responsible for over 880000 deaths each year.Growth/differentiation factor 15(GDF-15)is reported to be a promising diagnostic and prognostic factor in CRC.It induces pleiotropic effects in tumor cells:proliferation,sternness,invasion and metastasis.Some studies indicate that GDF-15 may stimulate angiogenesis in malignant neoplasms.However,it has not been investigated in CRC yet.The aim of our study was to determine the level of GDF-15 and the concentrations of hypoxia-inducible factor-la(HIF-1α),VEGF-A and chemokine-like receptor 1(CMKLR1)in tumor and margin specimens of CRC in relation to histological grade and TNM staging.The study comprised 33 samples of tumor and margin tissues obtained from CRC patients.To assess the concentration of GDF-15,HIF-1α,VEGF-A and CMKLR1,commercially available enzyme-linked immunosorbent assay(ELISA)kits were used.We found significantly increased levels of GDF-15 and CMKLR1 in tumor tissue compared to margin tissue and higher concentrations of HIF-1α and VEGF-A in margin tissue than in tumor tissue.The levels of GDF-15 and HIF-1α were significantly correlated with VEGF-A and CMKLR1 in margin tissue.In CRC,the increased level of GDF-15 might stimulate angiogenesis through upregulation of HIF-1α,VEGF A and CMKLR1 expression.Our study is the first one to reveal the correlation between the levels of GDF-15 and CMKLR1 in CRC.The elevated levels of HIF-1α and VEGF-A in tumor-free margin tissues suggest that noncancer cells in the tumor microenvironment are an important source of proangiogenic factors. | Sylwia Mielcarska Kamila Stopifnska Miriam Dawidowicz Agnieszka Kula Pawel Kicamer Alicja Prawdric Senkowska Ewa Nowakowska Zajde Katarzyna Walkiewicz Dariusz Waniczek Elzbieta Swietochowska | 2021 | Current Medical Science2021，41，3： | 3 |
| 20 | Bax protein may influence the invasion of colorectal cancer显示文摘AIM: To evaluate the expression of Bcl-xL, Bak, and Bax proteins in correlation with particular clinico-histopathological parameters, including tumor invasion front, in patients with colorectal cancer. METHODS: The expression of these proteins was evaluated with the use of the immunohistochemical method in 50 primary tumors. RESULTS: According to observations, a low expression of Bax and Bak proteins is related to the localization of the tumor in the rectum(P < 0.05 and P < 0.05 respectively), which may explain an increased incidence of colorectal cancer in this area. A positive expression of Bax protein also correlates with the presence of cancer cell infiltration to lymph and blood vessels(P < 0.05), which may suggest the participation of this protein in the early stages of colorectal cancer progression. Moreover, a positive expression of Bcl-xL protein correlated with a positive expression of Bak protein. This may suggest a greater participation of Bcl-xL protein in the inhibition of the proapoptotic Bak protein, but not the Bax protein. CONCLUSION: Bax protein is probably very significant in the cancerogenesis mechanism in the large intestine. | Anna Pryczynicz Mariusz Gryko Katarzyna Niewiarowska Dariusz Cepowicz Marek Ustymowicz Andrzej Kemona Katarzyna Guzińska-Ustymowicz | 2014 | World Journal of Gastroenterology2014，20，5： | 3 |
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