3篇
您的检索式：作者名="Daryush Talei"
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| 1 | Nain-e Havandi Andrographis paniculata present yesterday, absent today: a plenary review on underutilized herb of Iran’s pharmaceutical plants显示文摘 | Alireza Valdiani Mihdzar Kadir Soon Tan Daryush Talei Mohd Abdullah Sonia Nikzad | 2012 | Molecular Biology Reports2012，，5： | 1 |
| 2 | Micro- satellite-based evidences of genetic bottlenecks in the cryptic spe- cies 'Andrographis paniculata Nees': a potential anticaneer agent 显示文摘 | Alireza Valdiani Arash Javanmard Daryush Talei | 2013 | Mol Biol Rep2013，40，2： | 1 |
| 3 | Adjuvant role of Pseudomonas flagellin for Acinetobacter baumannii biofilm associated protein显示文摘AIM To study immunogenicity of Pseudomonas N terminal flagellin as an adjuvant for Acinetobacter baumannii(A. baumanni) biofilm associated protein(Bap).METHODS The N terminal flagellin gene was amplified. The p ET28a(+) and polymerase chain reaction products weredigested with HindⅢ and Eco R Ⅰ. The ligation of N terminal flagellin into p ET28a(?+) was performed using T4 DNA ligase and was then transformed into Escherichia coli BL21(DE3) as a suitable expression host. p ET28a(?+) vector harboring a conserved region of Bap from our previous work was used. The recombinant proteins were expressed, analyzed by SDS-PAGE method and was purified by affinity chromatography with His-Tag residues followed by confirmation with western blotting. Mice were immunized with recombinant N terminal flagellin and Bap subunits. The immunized animals were intranasally(i.n) challenged with A. baumannii and Pseudomonas aeruginosa(P. aeruginosa).RESULTS The flagellin enhanced the immunogenicity of Bap causing an increase in specific Ig G titers in serum(P < 0.001). Internal organs, i.e., liver, lung and spleen of the BapFlagellin immunized group challenged with A. baumannii showed significantly lower bacterial load compared to the control group. The bacterial loads were studied in internal organs. A. baumannii infected immunized animals with Bap-Flagellin exhibited internal organs with minor bacterial load while P. aeruginosa PAO1 infected group showed heavy bacterial load of(4.3 ± 0.12) × 106,(1.1 ± 0.01) × 106 and(2.2 ± 0.22) × 106 per gram of lungs, liver and spleen respectively. Bacterial loads were detected per gram of lungs, liver and spleen of the mice group immunized with Bap were(1.2 ± 0.06) × 107,(11.1 ± 0.041) × 105 and(3.6 ± 0.42) × 106 respectively. In vivo neutralization assay indicated that all experimental mice groups, except for Flagellin administered group was significantly(P < 0.05) protected against A. baumannii. CONCLUSION These results demonstrate that P. aeruginosa Flagellin as an adjuvant for Bap A. baumannii could be a useful model to evaluate new vaccine against A. baumannii. | Mozhgan Derakhshan Sefidi Iraj Rasooli Parviz Owlia Daryush Talei Shakiba Darvish Alipour Astaneh Shahram Nazarian | 2016 | World Journal of Methodology2016，6，3： | 1 |
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