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| 1 | Levels of insecticide resistance to deltamethrin,malathion,and temephos,and associated mechanisms in Aedes aegypti mosquitoes from the Guadeloupe and Saint Martin islands(French West Indies)显示文摘Background:In the Guadeloupe and Saint Martin islands,Aedes aegypti mosquitoes are the only recognized vectors of dengue,chikungunya,and Zika viruses.For around 40 years,malathion was used as a mosquito adulticide and temephos as a larvicide.Since the European Union banned the use of these two insecticide molecules in the first decade of the 21st century,deltamethrin and Bacillus thuringiensis var.israelensis are the remaining adulticide and larvicide,respectively,used in Guadeloupe.In order to improve the management of vector control activities in Guadeloupe and Saint Martin,we investigated Ae.aegypti resistance to and mechanisms associated with deltamethrin,malathion,and temephos.Methods:Ae.aegypti mosquitoes were collected from six different localities of Guadeloupe and Saint Martin.Larvae were used for malathion and temephos bioassays,and adult mosquitoes for deltamethrin bioassays,following World Health Organization recommendations.Knockdown resistance(Kdr)genotyping for V1016I and F1534C mutations,and expression levels of eight enzymes involved in detoxification mechanisms were examined in comparison with the susceptible reference Bora Bora strain.Results:Resistance ratios(RR50)calculated for Ae.aegypti larvae showed high resistance levels to temephos(from 8.9 to 33.1-fold)and low resistance levels to malathion(from 1.7 to 4.4-fold).Adult females displayed moderate resistance levels to deltamethrin regarding the time necessary to affect 50%of individuals,varying from 8.0 to 28.1-fold.Molecular investigations on adult mosquitoes showed high resistant allele frequencies for V1016I and F1534C(from 85 to 96%and from 90 to 98%,respectively),as well as an overexpression of the glutathione S-transferase gene,GSTe2,the carboxylesterase CCEae3a,and the cytochrome genes 014614,CYP6BB2,CYP6M11,and CYP9J23.Conclusions:Ae.aegypti populations from Guadeloupe and Saint Martin exhibit multiple resistance to organophosphates(temephos and malathion),and pyrethroids(deltamethrin).The mechanisms associated with these resistance patterns show strong frequencies of F1534C and V1016I Kdr mutations,and an over-expression of CCEae3a,GSTe2,and four cytochrome P450 genes(014614,CYP9J23,CYP6M11,CYP6BB2).These results will form the baseline for a deeper understanding of the insecticide resistance levels and associated mechanisms of Ae.aegypti populations and will be used to improve vector control strategies in Guadeloupe and Saint Martin. | Daniella Goindin Christelle Delannay Andric Gelasse Cedric Ramdini Thierry Gaude Frederic Faucon Jean-Philippe David Joël Gustave Anubis Vega-Rua Florence Fouque | 2017 | Infectious Diseases of Poverty2017,6,1: | 3 |
| 2 | Metallographic Methods for Revealing the Multiphase Microstructure of TRIP-Assisted Steels显示文摘 | E. Girault P. Jacques Ph. Harlet K. Mols J. Van Humbeeck E. Aernoudt F. Delannay | 1998 | Materials Characterization1998,,2: | 2 |
| 3 | Relative genetic contribution among ancestral lines to Noah American soybean cultivars显示文摘 | Delannay X Rodgers D M Palmer R G | 1983 | Crop Science1983,23,: | 1 |
| 4 | Development, identification and characterization of a glyphosate - tolerant soybean line 显示文摘 | Padgette S R Kolacz K H Delannay X D | 1995 | Crop Sci1995,35,: | 1 |
| 5 | Review the wetting of solids by molten metals and its relation to the preparation of metal matrix composites显示文摘 | DELANNAY F FROYEN L DERUYTTEE A | 1987 | J Mater Sci1987,,22: | 1 |
| 6 | Field-amplified sample stacking for the detection of chemical warfare agent degradation products in low-conductivity matrices by capillary electrophoresis-mass spectrometry显示文摘 | Lagarrigue M Bossee A Begos A Delannay N | 2008 | Journal of Chromatography A2008,,1178: | 1 |
| 7 | Enhancement of the mechanical properties of a low-carbon, low-silicon steel by formation of a multiphased microstructure containing retained Austenite显示文摘 | P. Jacques F. Delannay X. Cornet Ph. Harlet J. Ladriere | 1998 | Metallurgical and Materials Transactions A1998,,9: | 1 |
| 8 | Quantitative prediction of textures in aluminium cold rolled to moderate strains 显示文摘 | DELANNAY L KALID1NDI S R van HOUTTE P | 2002 | Materials Science and Engineering A2002,336,12: | 1 |
| 9 | Slow relaxation and compaction of granular systems显示文摘 | RICHARD P NICODEMI M DELANNAY R | 2005 | Nature Materials2005,4,2: | 1 |
| 10 | Development,identification and characterization of a glyphosate - tolerant soybean line 显示文摘 | Padgette S R Kolacz K H Delannay X | 1995 | Crop Science1995,35,: | 1 |
| 11 | Review the wetting of solids by molten metals and its relation to the preparation of metal-matrix composites显示文摘 | FROYEN L DERUYTTERE A | 1987 | Journal of Materials Science1987,22,: | 1 |
| 12 | Yield evaluation of a glyphosate-tolerant soybean line after treatment with glyphosate 显示文摘 | DELANNAY X BAUMAN T T BEIGHLEY D H | 1995 | Crop Science1995,35,5: | 1 |
| 13 | Relative contribution among ancestral lines to North American soybean cultivars显示文摘 | Delannay X Rodgers D M Palmer R G | 1983 | Crop Science1983,23,: | 1 |
| 14 | On the adhesion of the cohesive zone properties of an adhesive layer from the analysis of the wedge peel test显示文摘 | Ferracin T Landis C Delannay F | 2003 | Solids Struct2003,,40: | 1 |
| 15 | Development, Identification, and Charaterization of Glyphaste- tolerant Soybean Line显示文摘 | PADGETTE S R KOLAC K H DELANNAY X | 1995 | Crop Sci1995,35,: | 1 |
| 16 | The wetting of solids by molten metals and its relation to the preparation of metal-matrix composites composites显示文摘 | F. Delannay L. Froyen A. Deruyttere | 1987 | Journal of Materials Science1987,,1: | 1 |
| 17 | Thickness dependence of cracking resistance in thin aluminium plates显示文摘 | Pardoen T Marchal Y Delannay F | 1999 | Journal of the Mechanics and Physics of Solids1999,47,10: | 1 |
| 18 | Molecular breeding in developing countries : challenges and perspectives 显示文摘 | Ribaut J M de Vicente M C Delannay X | 2010 | Current O- pinion in Plant Biology2010,13,2: | 1 |
| 19 | The determinants of trade credit in transition countries显示文摘 | DELANNAY A F WEILL L | 2004 | Economics of Planning2004,,37: | 1 |
| 20 | Field performance of transgenic tomato plants expressing the Baeillusthuringiensis var Kurstaki insect contral protein显示文摘 | Delannay X Lavallee BJ Proksch R | 1989 | Biotechnolcgy1989,,7: | 1 |