| 4 | Impact of hormonal modulation at proestrus on ovarian responses and uterine gene expression of suckled anestrous beef cows显示文摘Background: This study evaluated the impact of hormonal modulation at the onset of proestrus on ovarian response and uterine gene expression of beef cows.Methods: A total of 172 anestrous beef cows were assigned to one of four groups according to the treatment with estradiol cypionate(ECP) and/or equine chorionic gonadotropin(eCG) [CON(n = 43), ECP(n = 43), eCG(n = 44)and ECP + eCG(n = 42)].Results: ECP-treated cows(ECP and ECP + eCG groups) presented greater occurrence of estrus(44.6% vs. 65.4%; P = 0.01) and pregnancy per AI [47.1% vs. 33.3%; P = 0.07], but similar progesterone(P4) concentration at subsequent diestrus than cows not treated with ECP(CON and eCG groups). Nonetheless, eCG-treated cows(eCG and ECP + eCG groups) presented larger follicle at timed AI(12.6 ± 0.3 vs. 13.5 ± 0.3 mm; P = 0.03), greater ovulation rate(96.5%vs. 82.6%; P = 0.008) and greater P4 concentration at d 6(3.9 ± 0.2 vs. 4.8 ± 0.2 ng/mL; P = 0.001) than cows not treated with eCG(CON and ECP groups). Next, cows with a new corpus luteum 6 d after TAI were submitted to uterine biopsy procedure. Uterine fragments [CON(n = 6), ECP(n = 6)] were analyzed by RNA-Seq and a total of 135 transcripts were differentially expressed between groups(73 genes up-regulated by ECP treatment). Subsequently,uterine samples were analyzed by q PCR(genes associated with cell proliferation). ECP treatment induced greater abundance of PTCH2(P = 0.07) and COL4 A1(P = 0.02), whereas suppressed EGFR(P = 0.09) expression. Conversely, eCG treatment increased abundance of HB-EGF(P = 0.06), ESR2(P = 0.09), and ITGB3(P = 0.05), whereas it reduced transcription of ESR1(P = 0.05). Collectively, supplementation with ECP or eCG at the onset of proestrous of anestrous beef cows influenced ovarian responses, global and specific endometrial gene expression.Conclusion: Proestrus estradiol regulate the endometrial transcriptome, particularly stimulating proliferative activity in the endometrium. | Manoel Francisco de Sá Filho Angela Maria Gonella-Diaza Mariana Sponchiado Marcio Ferreira Mendanha Guilherme Pugliesi Roney dos Santos Ramos Sónia Cristina da Silva Andrade Gustavo Gasparin Luiz Lehmann Coutinho Marcelo Demarchi Goissis Fernando Silveira Mesquita Pietro Sampaio Baruselli Mario Binelli | 2018 | Journal of Animal Science and Biotechnology2018，9，1： | 0 |
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