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您的检索式：作者名="Deqin ZHAI"
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| 1 | Ochratoxin A induces abnormal tryptophan metabolism in the intestine and liver to activate AMPK signaling pathway显示文摘Background Ochratoxin A(OTA)is a mycotoxin widely present in raw food and feed materials and is mainly pro-duced by Aspergillus ochraceus and Penicillium verrucosum.Our previous study showed that OTA principally induces liver inflammation by causing intestinal flora disorder,especially Bacteroides plebeius(B.plebeius)overgrowth.However,whether OTA or B.plebeius alteration leads to abnormal tryptophan-related metabolism in the intestine and liver is largely unknown.This study aimed to elucidate the metabolic changes in the intestine and liver induced by OTA and the tryptophan-related metabolic pathway in the liver.Materials and methods A total of 30 healthy 1-day-old male Cherry Valley ducks were randomly divided into 2 groups.The control group was given 0.1 mol/L NaHCO3 solution,and the OTA group was given 235μg/kg body weight OTA for 14 consecutive days.Tryptophan metabolites were determined by intestinal chyme metabolomics and liver tryptophan-targeted metabolomics.AMPK-related signaling pathway factors were analyzed by Western blot-ting and mRNA expression.Results Metabolomic analysis of the intestinal chyme showed that OTA treatment resulted in a decrease in intesti-nal nicotinuric acid levels,the downstream product of tryptophan metabolism,which were significantly negatively correlated with B.plebeius abundance.In contrast,OTA induced a significant increase in indole-3-acetamide levels,which were positively correlated with B.plebeius abundance.Simultaneously,OTA decreased the levels of ATP,NAD+and dipeptidase in the liver.Liver tryptophan metabolomics analysis showed that OTA inhibited the kynurenine metabolic pathway and reduced the levels of kynurenine,anthranilic acid and nicotinic acid.Moreover,OTA increased the phosphorylation of AMPK protein and decreased the phosphorylation of mTOR protein.Conclusion OTA decreased the level of nicotinuric acid in the intestinal tract,which was negatively correlated with B.plebeius abundance.The abnormal metabolism of tryptophan led to a deficiency of NAD+and ATP in the liver,which in turn activated the AMPK signaling pathway.Our results provide new insights into the toxic mechanism of OTA,and tryptophan metabolism might be a target for prevention and treatment. | Weiqing Ma Yang Fu Shanshan Zhu Daiyang Xia Shuangshuang Zhai Deqin Xiao Yongwen Zhu Michel Dione Lukuyu Ben Lin Yang Wence Wang | 2023 | Journal of Animal Science and Biotechnology2023，14，6： | 0 |
| 2 | Research on Acidic Stability of Immobilized Heavy Metals in Sediment from Polluted River显示文摘The heavy metals can accumulate in sediment after discharged into surface water bodies. Before the sediment can be further landfilled or recycled,a large amount of heavy metal in it needs to be treated,known as solidification/stabilization. The properties of treated sediment may change with environment such as acid rain and landfill time,which may lead to the release of heavy metals from treated sediment into environment,posing great risks to environment and human health. In this study,the quantitative relationship has been tested between the dissolution of heavy metals from treated sediment and the p H of the leaching solution,by changing the p H of the treated sediment-leaching solution continuously. The results showed that when the p H of the treated sediment-leaching solution was higher than 5. 5,the heavy metals released from the treated sediment into solution were relatively low;when the p H of the treated sediment-leaching solution dropped to about 5. 5,a large amount of Cu,Zn,Ni and other heavy metals were released. The solidification of Cu,Zn,Cr and Cd in the treated sediment is relatively stable,while that of Ni and Pb still need to be improved. The buffer capacity of the treated sediment to acid is 0. 999 meq/g,which means it has relatively high capacity to buffer the pH change caused by external acid. | Deqin ZHAI Dingpeng XIONG Yuxi YANG Shuo TAN | 2020 | Meteorological and Environmental Research2020，11，2： | 0 |
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