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11篇 您的检索式:作者名="Dexian Dong"
    题名 作者 年代 出处 被引量
1A DELLA protein complex controls the arbuscular mycorrhizal symbiosis in plants显示文摘Nan Yu Dexian Luo Xiaowei Zhang Junzhong Liu Wanxiao Wang Yue Jin Wentao Dong Jiyun Liu Huan Liu Weibing Yang Longjun Zeng Qun Li Zuhua He Giles E D Oldroyd Ertao Wang 2014Cell Research2014,24,1:9
2Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast显示文摘Tumor necrosis factor-related apop-tosis-inducing ligand (TRAIL) induces selectively apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. A chloroplast expres-sion vector, p64TRAIL, containing the cDNA coding for the soluble TRAIL (sTRAIL), was constructed with clpP-trnL-petB-chlL-rpl23-rpl2 as Chlamydomonas reinhardtii plastid homologous recombinant frag-ments and spectinomycin-resistant aadA gene as a select marker. The plasmid p64TRAIL was trans-ferred into the chloroplast genome of C. reinhardtii by the biolistic method. Three independently trans-formed lines were obtained by 100 mg/L spectino-mycin selection. PCR amplification, Southern blot analysis of the sTRAIL coding region DNA and culti-vation cells in the dark all showed that the exogenous DNA had been integrated into chloroplast genome of C. reinhardtii. Western blot analysis showed that human soluble TRAIL was expressed in C. reinhardtii chloroplast. The densitometric analysis of Western blot indicated that the expressed human sTRAIL protein in the chloroplasts of C. reinhardtii accounted for about 0.43%―0.67% of the total soluble proteins. These experimental results demonstrated the possi-bility of using transgenic chloroplasts of green alga as bioreactors for production of biopharmaceuticals.YANG Zongqi LI yinü CHEN Feng LI Dong ZHANG Zhifang LIU Yanxin ZHENG Dexian WANG Yong SHEN Guifang 2006Chinese Science Bulletin2006,51,14:4
3Treatment of acid rose dye containing wastewater by ozonizing – biological aerated filter显示文摘Xiaojun Wang Xiaoyang Gu Dexian Lin Fang Dong Xiaofang Wan 2006Dyes and Pigments2006,,3:3
4Removal of humic substances from soil DNA using aluminum sulfate 显示文摘DONG Dexian YANG An LI Haiming 2006J Microb Meth2006,66,:1
5Using trifluoroacetic acid to pretreat lignocellulosic biomass显示文摘Dong Dexian Sun Jie Huang Feiyun 2009biomass and bioenergy2009,,33:1
6Removal of humic substances from soil DNA using aluminium sulfate显示文摘DONG DEXIAN YAN AN LIU HAIMING 2006J Microbiol Methods2006,66,2:1
7Removal of humic substances from soil DNA using aluminum sulfate显示文摘DONG Dexian YANG An LI Haiming 2006J Microb Meth2006,66,:1
8Removal of humic substances from soil DNA using aluminium sulfate显示文摘Dexian Dong An Yan Haiming Liu Xuehong Zhang Yuquan Xu 2005Journal of Microbiological Methods2005,,2:1
9Cloning and identification of a novel binding protein of CD2 cytoplasmic domain显示文摘In order to understand why CD2 has a dual action of transduction of activation or apoptosis signals in T cells under different experimental conditions, we employed a yeast two-hybrid system to look for a binding protein of the cytoplasmic domain of CD2 which may be involved in this issue. A human T cell cDNA library was screened by a cDNA encoding the cytoplasmic domain of CD2 (Thr211-Gln336). The specificity of protein-protein interaction was verified by co-immunoprecipitation. The binding protein obtained, designated CD2cBP, was found to be homologous to v-fos transformation effector protein (Fte-1). As Fte-1 plays a role in cell transformation, growth, protein synthesis and protein-import into mitochondria, this result suggests that CD2cBP may be putatively involved in CD2-mediated signaling.SHAO Yuan XIAO Sheng ZHAI Wenjing LI Dong LIU Yanxin LIU Shilian ZHENG Dexian 2000Chinese Science Bulletin2000,45,24:1
10Genetic diversity of phenazine- and pyoluteorin-producing pseudomonads isolated from green pepper rhizosphere显示文摘Haiming Liu Dexian Dong Huasong Peng Xuehong Zhang Yuquan Xu 2006Archives of Microbiology2006,,2:1
11Biomimetic affinity purification of cardiotoxin and its pharmacological effects on the nervous systemy显示文摘Dexian Dong Haoran Liu Qishi Xiao 2008J Mol Recognit2008,21,:1
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