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| 1 | Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs显示文摘Background: During sperm maturation, there is a reorganization of fatty acids from plasmatic membrane of the spermatozoa, which allows higher membrane integrity and acquisition of sperm motility. However, the fatty acid profile during sperm maturation remains unclear in dogs. Thus, the aim of this study was to identify the fatty acids from the epididymal spermatozoa and plasma during the sperm maturation, and observed changes in the motility and plasmatic membrane parameters. Twenty one adult dogs were used, subsequently to bilateral orchiectomy and epididymal storage, sperm samples were collected from the different segments of the epididymis. Samples were evaluated for conventional microscopy, computer-assisted motility analysis, sperm plasma membrane permeability and the fatty acid analysis(lipids were extracted, transmethylated and analyzed by chromatography).Results: Caput and corpus sperm showed lower values for the motility variables evaluated and plasmatic membrane integrity, indicating different levels of the fatty acids organization. Saturated, monounsaturated and polyunsaturated fatty acids were in higher concentrations in the spermatozoa from epididymis cauda. Highlighting the presence of caprylic, stearic and docosahexaenoic acids.Conclusions: These findings demonstrate the influence of the fatty acid profile during sperm maturation, assigning physical and chemical changes in sperm cells, essential for fertilization. | Daniel S.Ramos Angrimani Marcilio Nichi Joāo Diego A.Losano Cristina F.Lucio Gisele A.Lima Veiga Márcia V.M.Junqueira Franco Camila I.Vannucchi | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 3 |
| 2 | Molecular genetics of berry colour variation in table grape显示文摘 | Diego Lijavetzky Leonor Ruiz-García José A. Cabezas María T. Andrés Gemma Bravo Ana Ibá?ez Juan Carre?o Félix Cabello Javier Ibá?ez José M. Martínez-Zapater | 2006 | Molecular Genetics and Genomics2006，，5： | 2 |
| 3 | 策略将在肝移植以后减少丙肝病毒复发显示文摘 Hepatitis C virus (HCV) is a major health problem that leads to chronic hepatitis, cirrhosis and hepatocellular carcinoma, being the most frequent indication for liver transplantation in several countries. Unfortunately, HCV re-infects the liver graft almost invariably following reperfusion, with an accelerated history of recurrence, leading to 10%-30% of patients progressing to cirrhosis within 5 years of transplantation. In this sense, some groups have even advocated for not retransplanting this patients, as lower patient and graftoutcomes have been reported. However, the management of HCV recurrence is being optimized and several strategies to reduce post-transplant recurrence could improve outcomes, decrease the rate of re-transplantation and optimize the use of available grafts. Three moments may be the focus of potential actions in order to decrease the impact of viral recurrence: the pretransplant moment, the transplant environment and the post-transplant management. In the pre-transplant setting, it is not well established if reducing the pre transplant viral load affects the risk for HCV progression after transplant. Obviously, antiviral treatment can render the patient HCV RNA negative post transplant but the long-term benefit has not yet been fully established to justify the cost and clinical risk. In the transplant moment, factors as donor age, cold ischemia time, graft steatosis and ischemia/reperfusion injury may lead to a higher and more aggressive viral recurrence. After the transplant, discussion about immunosuppression and the moment to start the treatment (prophylactic, pre-emptive or once-confirmed) together with new antiviral drugs are of interest. This review aims to help clinicians have a global overview of posttransplant HCV recurrence and strategies to reduce its impact on our patients. | Ruben Ciria María Pleguezuelo Shirin Elizabeth Khorsandi Diego Davila Abid Suddle Hector Vilca-Melendez Sebastian Rufian Manuel de la Mata Javier Briceo Pedro López Cillero Nigel Heaton | 2013 | World Journal of Hepatology2013，5，5： | 2 |
| 4 | Treatment and posttreatment effects of mandibular cervical headgear followed by fixed appliances in Class Ⅲ malocclusion显示文摘 | DIEGO R DAVID A GIOVANNI O | 2008 | Am J Orthod Dentofacial Orthnp2008，133，3： | 1 |
| 5 | Mapping wound-response genes involved in post-harvest physiological deterio-ration(PPD)of cassava(Manihot esculenta Crantz)显示文摘 | DIEGO F C KIM R EMMANUEL O | 2002 | Euphytica2002，128，： | 1 |
| 6 | Evaluation of phytotoxicity and antifungal activity against Colletotrichum acutatumof essential oils of thyme(Thymus vulgaris),lemongrass(Cymbopogon citratus),and its main constituents显示文摘 | Alzate O Diego A Mier M | 2009 | Vitae2009，16，1： | 1 |
| 7 | A genotype-phenotype correlation study in a group of 54 patients with X-linked agammaglobulinemia 显示文摘 | L o pez-Granados E P o fez de Diego R Ferreira Cerd a n A | 2005 | J Allergy Clin Immunol2005，116，3： | 1 |
| 8 | Production of matrix metalloproteinnase-9 in lipopolysaccharide-stimulated human amnion occurs through an autocrine and paracrine proinflammatory cytokine-dependent system显示文摘 | FABIAN A V DIEGO O SAMUEL P | 2002 | Biol Reprod2002，67，： | 1 |
| 9 | Silencing of DELLA induces facultative parthenocarpy in tomato fruits 显示文摘 | Cristina M Diego O Philippe E | 2007 | Plant J2007，52，： | 1 |
| 10 | Antifouling technology - past, present and future steps towards efficient and environmentally friend- ly antifouling coatings 显示文摘 | DIEGO M Y S O REN K KIM D J | 2004 | Progress in Organic Coatings2004，50，2： | 1 |
| 11 | A practical use of CFD for ventilation of underground works显示文摘 | DIEGO I TORNO S TORA O J | 2011 | Tunnelling and Underground Space Technology2011，，26： | 1 |
| 12 | Exploing the natural and human-induced effects on the assemblage of soil microarthropod communities inArgentina显示文摘 | Diego O Ferraro Qaudio M Ghersa | 2007 | European Journal of soil Biology2007，43，2： | 1 |
| 13 | A simple mathematical modea of cooperativity in receptor mosaics based on the 'symmetry rule'显示文摘 | LUIGI F A ALEXANDER O T DIEGO G | 2005 | Biosystems2005，80，： | 1 |
| 14 | Emerging views on the distinct but related roles of the main and accessory olfactory systems inresponsiveness to chemosensory sig- nals in mice显示文摘 | Diego R Julie A Anthony M O | 2002 | Hormones and Behavior2002，26，： | 1 |
| 15 | Exploring the natural and human-in- duced effects on the assemblage of soil microarthropod com- munities in Argentina显示文摘 | Diego O F Qaudio M G | 2007 | European Journal of Soil Biology2007，43，2： | 1 |
| 16 | Plants and human health in the twenty-first century显示文摘 | Ilya Raskin David M. Ribnicky Slavko Komarnytsky Nebojsa Ilic Alexander Poulev Nikolai Borisjuk Anita Brinker Diego A. Moreno Christophe Ripoll Nir Yakoby Joseph M. O'Neal Teresa Cornwell Ira Pastor Bertold Fridlender | 2002 | Trends in Biotechnology2002，，12： | 1 |
| 17 | The burden of community-acquired pneumonia in the eldrely: the Spanish EVAN-65 study显示文摘 | OCHOA-GONDER O VILA-CORCOLES A de DIEGO C | 2008 | BMC Public Health2008，8，： | 1 |
| 18 | Exploing the natural and human-induced effects on the assemblage of soil microarthropod communities in Argentina显示文摘 | DIEGO O F QAUDIO M G | 2007 | European Journal of Soil Biology2007，43，2： | 1 |
| 19 | FEM models including randomness and its application to the blasting vibrations prediction显示文摘 | TORA(N)O J RODR(I)GUEZ R DIEGO I | | 0，，01： | 1 |
| 20 | C-reactive protein elevation and early outcome in patients with unstable angina pectoris显示文摘 | Luigi O Diego A Piera AM | 1997 | Am J Cardiol1997，80，： | 1 |
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