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| 1 | Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology显示文摘Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field. | Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang | 2021 | Engineering2021，7，9： | 4 |
| 2 | Pricing and inventory decisions for non-instantaneous deteriorating items with price and promotional effort stochastic demand显示文摘This study investigates an inventory model for a non-instantaneous deteriorating item with partial backlogging wherein the demand is stochastic in nature and depends on price and promotional effort whereas the deterioration rate is time proportional.Under these settings,a mathematical model is developed with the objective to maximise the expected profit per unit time by determining the optimal price and the length of replenishment cycle.Some useful theoretical results are established to deduce the optimal replenishment schedule.An effective algorithmic procedure is developed to find the optimal solutions to the proposed model.The applicability of the proposed model is illustrated by numerical example.Sensitivity analysis of the optimal solution with respect to key parameters has been carried out and the implications are discussed.The study indicates that though promotional effort stimulates the market demand,it is beneficial in an economic sense when it applies in a conservative manner. | Hardik N.Soni Dipali N.Suthar | 2019 | Journal of Control and Decision2019，6，3： | 2 |
| 3 | Epidemiology of osteoarthritis:an update显示文摘 | Leena Sharma Dipali Kapoor Sakeba Issa | 2006 | Curr Opin Rheum2006，18，2： | 1 |
| 4 | Epidemiology of osteoarthritis: an update 显示文摘 | Leena S Dipali K Sakeba I | 2006 | Curr Opin Rheum2006，18，2： | 1 |
| 5 | Mathematical Modeling and Experimental Study on Thin-Layer Vacuum Drying of Ginger (Zingiber Officinale R.) Slices显示文摘 | Indrajit Thorat Debabandya Mohapatra R. Sutar S. Kapdi Dipali Jagtap | 2012 | Food and Bioprocess Technology2012，，4： | 1 |
| 6 | Epidemiology of osteoarthristis:an update显示文摘 | LEENA SHARMA DIPALI KAPOOR SAKEBA ISSA | 2006 | Curr Opin Rheum2006，，： | 1 |
| 7 | Comparative Study of Separation of Non - encapsulated Drug from Unilamellar Liposomes by Various Methods显示文摘 | DIPALI S R Kulkavni S B Betageri G V | 1996 | J Pharm Pharmacol1996，48，11： | 1 |
| 8 | Polyaniline based electrodes for electrochemical supercapacitor: Synergistic effect of silver, activated carbon and polyaniline显示文摘 | Dipali S P Pawar S A Devan R S | 2014 | Journal of Electroanalytical Chemistry2014，724，： | 1 |
| 9 | Fabrication of sucrose biosensor based on single mode planar optical waveguide using co-immobilized plant invertase and GOD 显示文摘 | Dipali S B Anu V Aiyer R C | 2007 | BiosensBioelectron2007，22，： | 1 |
| 10 | Invertase inhibition based electrochemical sensor for the detection of heavy metal ions in aqueous system: Application of ultra-microelectrode to enhance sucrose biosensor’s sensitivity显示文摘 | Dipali Bagal-Kestwal Meena S. Karve Bhalchandra Kakade Vijayamohanan K. Pillai | 2008 | Biosensors and Bioelectronics2008，，4： | 1 |
| 11 | Comparative study of separation of non-encapsulated drug from unilamellar liposomes by various methods 显示文摘 | Dipali S R Kulkavni S B Betageri G V | 1996 | Pharm Pharmacol1996，48，11： | 1 |
| 12 | Epidemiology of osteoarthritis: an update显示文摘 | Leena Sharma Dipali Kapoor Sakeba Issa | 2006 | Current Opinion in Rheumatology2006，，2： | 1 |
| 13 | Epidemiology of osteoarthritis :an update显示文摘 | Leena Sharma Dipali Kapoor Sakeba Issa | 2006 | Curr Opin Rheum2006，18，2： | 1 |
| 14 | Comparative study of separation of non-encapsulated drug from unilamellar liposomes by various methods显示文摘 | Kulkavni SB Betageri GV | 1996 | J Pharm Pharmacol1996，48，11： | 1 |
| 15 | Complete cDNA sequence of bovine alpha 1-antitrypsin 显示文摘 | Dipali S Meenakshi R B Edward P K | 1992 | Biochimica et Biophysica Acta1992，1130，2： | 1 |
| 16 | Complete cDNA sequence of bovine alpha 1-antitrypsin显示文摘 | Dipali Sinha Meenakshi R Bakhshi Edward P Kirby | 1992 | Biochimica et Biophysica Acta1992，1130，2： | 1 |
| 17 | Epidemiology of osteoarthritis: an update 显示文摘 | Leena Sharma Dipali Kapoor Sakeba lssa | 2006 | Curr Opin Rheum2006，18，2： | 1 |
| 18 | Epidemiology of osteoarthritis:an update显示文摘 | Leena Sharma Dipali Kapoor Sakeba Issa | 2006 | Curr Opin Rheum2006，18，2： | 1 |
| 19 | Catalytic Ring Opening of MCH and MCP Over Ir Containing USY and HZSM-5 with the Same SiO2/Al2O3 Ratio显示文摘 | Dipali Upare Songhun Yoon Chul Lee | 2012 | Catalysis Letters2012，，6： | 1 |
| 20 | Pharmacokinetics and tissue distribution of long circulating liposomal formulation of 2′,3′-dideoxyinosine显示文摘 | Satish R. Dipali Yuh-Jing Lin William R. Ravis Guru V. Betageri | 1997 | International Journal of Pharmaceutics1997，，1： | 1 |
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