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| 1 | Multispecies probiotic protects gut barrier function in experimental models显示文摘AIM:To investigate the effect of the probiotic combination Lactibiane Tolerance?(LT)on epithelial barrier function in vitro and in vivo.METHODS:The effect of the multispecies probiotic LT was assessed on several models of epithelial barrier function both in vitro(in basal and inflammatory conditions)and in vivo[visceral hypersensitivity induced by chronic stress or by colonic perfusion of a fecal supernatant(FSN)from patients with irritable bowel syndrome(IBS)].In vitro,we measured the permeability of confluent T84 cell monolayers incubated with or without LT by evaluating the paracellular flux of macromolecules,in basal conditions and after stimulation with lipopolysaccharide(LPS)or with conditioned medium of colonic biopsies from IBS patients(IBS-CM).In vivo,male C57/Bl6 mice received orally NaCl or LT for 15 d and were submitted to water avoidance stress(WAS)before evaluating visceral sensitivity by measuring the myoelectrical activity of the abdominal muscle and the paracellular permeability with 51Cr-EDTA.Permeability and sensitivity were also measured after colonic instillation of FSN.Tight-junctions were assessed by immunoblotting and TLR-4 expression was evaluated by immunohistochemistry RESULTS:Incubation of T84 cell monolayers with LT in basal conditions had no significant effect on permeability(P>0.05 vs culture medium).By contrast,addition of LT bacterial bodies(LT)completely prevented the LPS-induced increase in paracellular permeability(P<0.01 vs LPS 10 ng/mL(LPS 10);P<0.01 vs LPS 100ng/mL(LPS 100),P>0.05 vs culture medium).The effect was dose dependent as addition of 109 LT bacterial bodies induced a stronger decrease in absorbance than 106 LT(109 LT+LPS 10:-20.1%±13.4,P<0.01vs LPS 10;106 LT+LPS 10:-11.6%±6.2,P<0.01 vs LPS 10;109 LT+LPS 100:-14.4%±5.5,P<0.01 vs LPS 100;106 LT+LPS 100:-11.6%±7.3,P<0.05 vs LPS 100).Moreover,the increase in paracellular permeability induced by culturing T84 cells with conditioned medium of colonic biopsies from IBS patients(IBS-CM)was completely inhibited in the presence of 109 LT(P<0.01 vs IBS-CM).LT also significantly prevented the epithelial disruption induced by intracolonic infusion of fecal supernatant from IBS patients(P<0.01 vs IBS FSN)or water avoidance stress P<0.01 vs WAS)in C57/Bl6 mice and increased the expression of occludin in vitro and in vivo,as assessed by immnunoblotting.The WAS-induced effect on visceral sensitivity was prevented by LT treatment since values obtained for all steps of colorectal distension were significantly(P<0.01)different from the WAS group.Finally,LT downregulated the response mediated through TLR-4 in vitro(decrease in tumor necrosis factorαsecretion in response to LPS:-65.8%for 109 LT and-52.5%for 106LT,P<0.01 vs LPS)and in vivo(inhibition of WAS induced an increase in TLR-4 expression in the LT treated mice colon,P<0.01 vs WAS).CONCLUSION:The probiotic LT mix prevented the disruption to the epithelial barrier induced by LPS,stress or colonic soluble factors from IBS patients and prevented visceral hypersensitivity. | Mylene Nébot-Vivinus Cherryl Harkat Hanene Bzioueche Christel Cartier Raffaella Plichon-Dainese Lara Moussa Helene Eutamene Dorsa Pishvaie Sophie Holowacz Christian Seyrig Thierry Piche Vassilia Theodorou | 2014 | World Journal of Gastroenterology2014，20，22： | 12 |
| 2 | Effects of acetic acid, lactic acid and trisodium phosphate on the microflora of refrigerated beef carcass surface tissue inoculated with Escherichia coli O157 : H7, Listeria innocua , and Clostridium s poro genes 显示文摘 | Dorsa W J Cutter C N Siragusa G R | 1997 | Journal of Food Protection1997，60，6： | 1 |
| 3 | Transcriptional effects of estrogen on neuronal neurotensin gene expression involve cAMP/protein kinase A-dependent signaling mechanisms显示文摘 | Watters JJ Dorsa DM | 1998 | J Neurosci1998，18，17： | 1 |
| 4 | Estrogen activates rapid signaling in the brain: role of estrogen receptor alpha and estrogen receptor beta in neurons and glia显示文摘 | Mhyre A J Dorsa D M | 2006 | Neuroscience2006，138，： | 1 |
| 5 | Roles of estrogen receptors alpha and beta in sexually dimorphic neuroprotect ion anainst glutam ate toxicity显示文摘 | Bryant DN Dorsa DM | 2010 | Neuroscience2010，170，： | 1 |
| 6 | Estrogen activates rapid signaling in the brain : role of estrogen receptor alpha and estrogen receptor beta in neurons and glia 显示文摘 | Mhyre A J Dorsa D M | 2006 | Neuroscience2006，138，3： | 1 |
| 7 | Estrogen receptor-mediated neuroprotection from oxidative stress requires activation of the mitogen-activated protein binase pathway显示文摘 | Mize AL Shapiro RA Dorsa DM | 2003 | Endoerindogy2003，144，： | 1 |
| 8 | Roles of estrogen receptors alpha and beta in sexually dimorphic neuroproteetion against glutamate toxicity显示文摘 | Bryant DN Dorsa DM | 2010 | Neuroscience2010，170，4： | 1 |
| 9 | 17β-Estradiol and the phytoestrogen genistein attenuate neuronal apoptosis induced by the endoplasmic reticulum calcium-ATPase inhibitor thapsigargin显示文摘 | Linford NJ Dorsa DM | 2002 | Steroids2002，67，1314： | 1 |
| 10 | A rapid microbial ATP bioluminescence assay for meat carcasses显示文摘 | Cutter C N Dorsa G R Siragusa | 1996 | Dairy Food Sanit1996，16，： | 1 |
| 11 | Estrogen receptor-mediated neuroprotection from oxidative stress requires activation of the mitugen-activated protein kinase pathway显示文摘 | Mize A Shapiro R Dorsa D | 2003 | Endocrinology2003，144，： | 1 |
| 12 | 17β-Estradiol and the phytoestrogen genistein attenuate neuronal apoptosis induced by the endoplasmic reticulum calcium-ATP inhibitor thapsigargin显示文摘 | Linford NJ Dorsa DM | 2002 | Steroids2002，67，： | 1 |
| 13 | Biolumin显示文摘 | RSiragusa G Dorsa W J Cutter C N | 1996 | Chemilumin1996，11，6： | 1 |
| 14 | Modulation of bcl-2 expres- sion: a potential component of estrogen protection in NT2 neu- rons显示文摘 | Singer CA Rogers KL Dorsa DM | 1998 | Neuroreport1998，9，： | 1 |
| 15 | Effects of dietary cottonseed meal and gossypol on growth of young channel catfish显示文摘 | Dorsa W J Robinette H R Robinson E H | 1982 | Transaction of the American Fisheries Society1982，3，： | 1 |
| 16 | Immediate extubation after off-pump coronary artery bypass graft surgery in 1,196 consecutive patients:feasibility,safety and predictors of when not to attempt it显示文摘 | Dorsa AG Rossi AI Thierer J | 2011 | J Cardiothorac Vasc Anesth2011，25，3： | 1 |
| 17 | The effects of ovariectomy and estrogen replacement on trkA and choline acetyltransferase mRNA expression in the basal forebrain of the adult female Sprague-Dawley rat 显示文摘 | Singer CA Dorsa DM | 1996 | J Neurosci1996，16，5： | 1 |
| 18 | The effects of ovariect-emy and estrogen replacement on trkA chorine acetyltransferase mRNA expression in the basal forebrain of the adult female Sprague-Dawley rat 显示文摘 | McMillan PJ singer CA Dorsa DM | 1996 | Neruosci1996，16，5： | 1 |
| 19 | Neuroprotective effects of estrogen and selective estrogen receptor modulators begin at the plasma membrane显示文摘 | Sheldahl L C Marriott L K Bryant D M Shapiro R A Dorsa D M | 2007 | Minerva Endocrinol2007，32，： | 1 |
| 20 | Insulin in the Brain显示文摘 | D G Baskin D P Figlewicz S C Woods D Porte D M Dorsa | 1987 | Annual Review of Physiology1987，，： | 1 |
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