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您的检索式：作者名="Duangkamol"
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| 1 | Development of species-specific SCAR markers for identification of three medicinal species of Phyllanthus显示文摘Phyllanthus amarus Schum. & Thonn. has been widely used in traditional medicine in Thailand as an antipyretic, a diuretic, to treat liver diseases and viral infections. Two closely related species, P. debilis L. and P. urinaria Klein ex Willd., with different and less effective medicinal properties, are less commonly used. These three species are similar in morphology and often occur in overlapping populations in nature. The latter two species can easily be mistaken for P. amarus and collected for medicinal uses, which can lead to undesirable results. DNA fingerprints of these species were obtained using RAPD-PCR techniques. RAPD markers specific for each species were identified. Primers for highly specific sequence-characterized-amplified-regions (SCAR) were then designed from nucleotide sequences of specific RAPD markers. These primers efficiently amplified SCAR markers of 408, 501 and 319 bp unique to P. amarus, P. debilis and P. urinaria respectively. This method of plant identification was rapid and highly specific when tested against DNA of several closely related species and was able to amplify specific markers from mixed DNA samples. | Piyada THEERAKULPISUT Nantawan KANAWAPEE Duangkamol MAENSIRI Sumontip BUNNAG Pranom CHANTARANOTHAI | 2008 | 植物分类学报2008，46，4： | 4 |
| 2 | A chalcone with potent inhibiting activity against biofilm formation by nontypeable Haemophilus influenzae显示文摘 | Duangkamol Kunthalert Sudarat Baothong Pichit Khetkam Suwadee Chokchaisiri Apichart Suksamrarn | 2014 | Microbiol Immunol2014，，10： | 1 |
| 3 | In vitro interference of cefotaxime at subinhibitory concentrations on biofilm formation by nontypeable Haemophilus influenzae显示文摘Objective:To investigate the in vitro interference of cefotaxime at subinhibitory concentrations [sub-minimal inhibitory concentrations(MIC)] on biofilm formation by nontypeable Haemophilus influenzae(NTHi).Methods:The interference of subinhibitory concentrations of cefotaxime on biofilm formation of the clinical strong-biofilm forming isolates of NTHi was evaluated by a microtiter plate biofilm formation assay.The effect of sub-MIC cefotaxime on bacterial cell-surface hydrophobicity was determined using a standard microbial adhesion to n-hexadecane test.Additionally,the effects on bacterial adherence to human fibronectin and expression of bacterial adhesins were also investigated.Results:Subinhibitory concentrations of cefotaxime,both at 0.1× and 0.5× MIC levels,efficiently reduced the NTHi biofilm formation,and this effect was independent of decreasing bacterial viability.Sub-MIC cefotaxime also decreased bacterial cell-surface hydrophobicity and reduced adherence to human fibronectin.Inhibition in the P2 and P6 gene expressions upon exposure to sub-MIC cefotaxime was also noted.Conclusions:Taken together,our results indicate that sub-MIC cefotaxime interferes with the formation of NTHi biofilm,and this effect is feasibly related to the interference with cell-surface hydrophobicity,fibronectin-binding activity as well as alteration of the P2 and P6 gene expression.The findings of the present study therefore provide a rationale for the use of subinhibitory concentrations of cefotaxime for treatment of NTHi-related diseases. | Sudarat Baothong Sutthirat Sitthisak Duangkamol Kunthalert | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，9： | 1 |
| 4 | Improved prediction of pile bending moment and deflection due to adjacent braced excavation显示文摘Deep excavations in dense urban areas have caused damage to nearby existing structures in numerous past construction cases.Proper assessment is crucial in the initial design stages.This study develops equations to predict the existing pile bending moment and deflection produced by adjacent braced excavations.Influential parameters(i.e.,the excavation geometry,diaphragm wall thickness,pile geometry,strength and small-strain stiffness of the soil,and soft clay thickness)were considered and employed in the developed equations.It is practically unfeasible to obtain measurement data;hence,artificial data for the bending moment and deflection of existing piles were produced from well-calibrated numerical analyses of hypothetical cases,using the three-dimensional finite element method.The developed equations were established through a multiple linear regression analysis of the artificial data,using the transformation technique.In addition,the three-dimensional nature of the excavation work was characterized by considering the excavation corner effect,using the plane strain ratio parameter.The estimation results of the developed equations can provide satisfactory pile bending moment and deflection data and are more accurate than those found in previous studies. | Chana PHUTTHANANON Pornkasem JONGPRADIST Duangkamol SIRIRAK Prateep LUEPRASERT Pitthaya JAMSAWANG | 2023 | Frontiers of Structural and Civil Engineering2023，17，11： | 0 |
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