|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa显示文摘Background: Lycopene(LYC) is a natural carotenoid with powerful reactive oxygen species(ROS) scavenging activities. The aim of this study was to investigate if lycopene has the ability to reverse ROS-mediated alterations to the motility, viability and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate(Fe AA). Spermatozoa were washed out of fresh bovine semen, suspended in 2.9 % sodium citrate and subjected to LYC treatment(0.25, 0.5, 1 or 2 mmol/L) in the presence or absence of Fe AA(150 μmol/L Fe SO_4 and 750 μmol/L ascorbic acid) during a 6 h in vitro culture. Spermatozoa motion characteristics were assessed using the Sperm Vision?computer-aided sperm analysis(CASA) system. Cell viability was examined with the metabolic activity(MTT) assay,ROS generation was quantified via luminometry and the nitroblue-tetrazolium(NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro culture to investigate the intracellular activity of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx) as well as the concentrations of glutathione(GSH) and malondialdehyde(MDA).Results: FeA A treatment led to a reduced spermatozoa motility(P < 0.001), viability(P < 0.001) and a decline of the antioxidant capacity of spermatozoa(P < 0.001) but increased the ROS generation(P < 0.001), superoxide production(P < 0.001) and lipid peroxidation(P < 0.001). LYC administration resulted in a preservation of the spermatozoa motion parameters(P < 0.001), mitochondrial activity(P < 0.001) and antioxidant characteristics(P < 0.001 with respect to SOD;P < 0.01 in relation to CAT; P < 0.05 as for GPx and GSH) with a concentration range of 1 and 2 mmol/L LYC revealed to be the most effective.Conclusions: Our results suggest that LYC exhibits significant ROS-scavenging and antioxidant properties which may prevent spermatozoa alterations caused by oxidative stress, and preserve the functionality of male reproductive cells. | Eva Tvrdá Anton Kovácik Eva Tusimová Dusan Paál Alica Mackovich Jakhongir Alimov Norbert Lukác | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 3 |
| 2 | Maternal trait anxiety,depression and life event stress in pregnancy:Relationships with infant temperament显示文摘 | Dusan H P Leo L | 2005 | Early Human Development2005，81，2： | 1 |
| 3 | Adsorption of Pb^2+ Cd^2+ Sr^2+ ions onto natural and acid-activated sepiolites显示文摘 | Slavica L Ivona J Dusan J | 2007 | Appl Clay Sci2007，37，1： | 1 |
| 4 | Adsorption of Pb2+,Cd2+ and Sr2+ ions onto natural and acid-activated sepiolites显示文摘 | Slavica L Ivona J Dusan J | 2007 | Applied Clay Science2007，37，1： | 1 |
| 5 | A role for IL-15 in driving the onset of spontaneous autoimmune thyroiditis显示文摘 | Kaiser P Rothwell L Dusan V | 2002 | J Immunol2002，168，： | 1 |
| 6 | Chlorophylla fluorescence induction显示文摘 | Dusan L | 1999 | Biochim Biophysiol Acta1999，14，12： | 1 |
| 7 | Mycoplasma synoviae invades non-phagocytic chicken cells in vitro显示文摘 | Dusanic' D Bercic R L Cizelj I | 2009 | Veterinary Microbiology2009，138，12： | 1 |
| 8 | Effect of Excess Air Ratio and Temperature on NOx Emission from Grate Combustion of Biomass in the Staged Air Combustion Scenario显示文摘 | Ehsan Houshfar (Q)dfyvind Skreiberg Terese L(Q) vas Dusan Todorovi Lars S (Q) rum | | 0，，： | 1 |
| 9 | 显示文摘 | Dusan L Justin G J | 1999 | Proc-Aust Conf-Nucl Tech Anal1999，11，： | 1 |
| 10 | Fractal Models of Elastic-perfectly Plastic Contact of Rough Surfaces Based on the Cantor Set显示文摘 | Thomas W Dusan Krajcinovic L | 1995 | International Journal of Solids and Structures1995，32，： | 1 |
| 11 | Mycoplasma synoviae invades non-phagocytic chicken cells in vitro 显示文摘 | Dusanic D Bercic R L Cizelj I | 2009 | Vet Mierobiol2009，138，12： | 1 |
| 12 | Computing with bees: attacking complex transportation engineering problems 显示文摘 | Panta L Dusan T | 2003 | International Journal on Artificial Intelligence Tools2003，12，3： | 1 |
| 13 | Adsorption of Pb^2+ , Cd^2+ and Sr^2+ ions onto natural and acid activated sepiolites 显示文摘 | Slavica L Ivona J C Dusan J | 2007 | Ap- plied Clay Science2007，37，12： | 1 |
| 14 | Left ventricular pacing thresholds with new cardiac vein lead compared with right ventricular thresholds 显示文摘 | Angel L David D Dusan ZK | 2000 | Circulation2000，102，18： | 1 |
| 15 | Nanoporous anodic alu- minium oxide: Advances in surface engineering and emer- ging applications 显示文摘 | Abul M M Dusan L Nicolas H V | 2013 | Prog Mater Sci2013，58，5： | 1 |
| 16 | High-temperature induced chlorophyll fluorescence changes in barley leaves显示文摘 | Petr I | 1997 | Plant Science1997，124，： | 1 |
| 17 | Maternal trait anxiety, de- pression and life event stress in pregnancy: relationships with infant temperament显示文摘 | Marie PA Dusan HP Leo L | 2005 | Early Hum Dev2005，81，： | 1 |
| 18 | Maternal trait anxiety,depression and life event stress in pregnancy:relationships with infant tempera ment显示文摘 | Marie PA Dusan HP Leo L | 2005 | Early Hum Dev2005，81，： | 1 |
| 19 | Self-ordering electrochemicalsynthesis of T1O2 nanotube arrays: controlling the nanotubegeometry and the growth rate显示文摘 | Krishna K Dusan L | 2011 | International Journal ofNanoscience2011，10，1： | 1 |
| 20 | High-temperature induced chlorophyll fluorescence changes in barely leaves显示文摘 | Dusan L Petr I | 1997 | Plant Science1997，124，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员