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| 1 | 荧光偏振免疫分析在农药和兽药残留检测中的研究进展显示文摘荧光偏振免疫分析技术是以免疫竞争原理和荧光偏振原理为基础的一种以快速筛选为目的的分析技术,主要用于测定小分子量物质(抗原),就是检测荧光标记抗原在结合特异性的抗体前后,荧光偏振值的变化,是均相的竞争免疫分析方法,反应系统完全在溶液中,不需要分离结合的和未结合的抗体,不受溶液颜色、仪器灵敏度变化的影响,该技术最显著的特点是操作简单和检测时间短,适用于大量样本的快速筛选检测。目前该技术在农药和兽药残留分析中已经得到了应用,但是国内的相关研究尚未见报道。文章综述了荧光偏振免疫分析的历史背景,发展历程和主要原理,概括了荧光偏振免疫分析方法在农药和兽药残留分析中的研究进展。 | 王战辉 张素霞 沈建忠 Sergei A Eremin | 2007 | 光谱学与光谱分析2007，27，11： | 19 |
| 2 | 荧光偏振免疫分析方法快速检测沙拉沙星残留显示文摘以异硫氰酸荧光素(FITC)标记沙拉沙星合成荧光标记物,采用薄层色谱法提纯,优化了反应时间、标记物和抗体的工作浓度,建立了沙拉沙星的快速荧光偏振免疫分析法(FPIA)。本方法测定沙拉沙星在缓冲液中的半数抑制浓度(IC50)为43.2μg/L;检测范围为5.7~327μg/L,可以达到国家规定的动物性食品中兽药最高残留限量(80μg/kg)的要求。本研究考察了FPIA测定沙拉沙星的动力学过程及对其它4种喹诺酮类药物的交叉反应。结果表明,环丙沙星、恩诺沙星、加替沙星及氧氟沙星的交叉反应率分别为3.3%,1.8%,1.7%和0.7%。在牛奶和猪尿中沙拉沙星的回收率分别在71%~94%和74%~102%之间。本方法操作简单快捷,整个检测过程只需5 min、而且灵敏度较高、特异性强,适用于动物性食品中沙拉沙星残留的快速筛选检测。 | 宋佩 孟萌 Sergei A Eremin 张太昌 田溪 薛虎寅 张昱 尹永梅 郗日沫 | 2012 | 分析化学2012，40，8： | 16 |
| 3 | The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection显示文摘A fluorescence polarization immunoassay(FPIA)was developed for the analysis ofaflatoxins(AFs)using an anti-aflatoxin B1(AFB1)monoclonal antibody and a novel fluorescein-labeled AFB1tracer.The FPIA showed an IC50 value of 23.33ng/mL with a limit of detection of 13.12 ng/mL for AFB1.The cross-reactivities of AFB1,AFB2,AFG1,AFG2,AFM1,and AFM2 with the antibody were100%,65.7%,143%,23.5%,111.4%,and 2%,respectively.The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs,albeit not AFG2 and AFM2.The total time required for analyzing 96 samples in one microplate was less than 5 min.This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs. | SHENG Ya Jie EREMIN Sergei MI Tie Jun ZHANG Su Xia SHEN Jian Zhong WANG Zhan Hui | 2014 | Biomedical and Environmental Sciences2014，27，2： | 10 |
| 4 | Numerical simulation of roof cavings in several Kuzbass mines using finite-difference continuum damage mechanics approach显示文摘An essential stage of mine design is an estimation of the steps of the first and periodic roof caving in longwall mines.Generally,this is carried out using the field experience and can be much enhanced by numerical simulation.In this work,the finite-difference method was applied coupled with the continuum damage mechanics(CDM)approach to simulate the stress-strain evolution of the rock mass with the underground opening during coal extraction.The steps and stages of roof caving were estimated relying on the numerical simulation data,and they were compared with the field data from several operating mines in the south of the Kuznetsk Basin,Russia.The dependence of the first roof caving step in simulation linearly correlates with field data.The results correspond to the actual roofs of longwall panels of the flat-dipping coal seams and the average rate of face advancement is approximately 5 m/day. | Mikhail Eremin Gabriel Esterhuizen Igor Smolin | 2020 | International Journal of Mining Science and Technology2020，30，2： | 3 |
| 5 | Detection of aflatoxin M1 in milk products from China by ELISA using monoclonal antibodies显示文摘 | Shi Chun Pei Yuan Yuan Zhang Sergei A. Eremin Won Jong Lee | 2009 | Food Control2009，，12： | 2 |
| 6 | Direct competitive ELISA based on a monoclonal antibody for detection of aflatoxin B1. Stabilization of ELISA kit components and application to grain samples显示文摘 | Anna Yu Kolosova Won-Bo Shim Zheng-You Yang Sergei A. Eremin Duck-Hwa Chung | 2006 | Analytical and Bioanalytical Chemistry2006，，1： | 2 |
| 7 | Development of anti-zearalenone monoclonal antibody and detection of zearalenone in corn products from China by ELISA显示文摘 | Shi-Chun Pei Won-Jong Lee Gai-Ping Zhang Xiao-Fei Hu Sergei A. Eremin Li-Jun Zhang | 2013 | Food Control2013，，1： | 2 |
| 8 | Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules显示文摘 | Smith DS Eremin SA | 2008 | Anal Bioanal Chem2008，391，： | 1 |
| 9 | Preoperative treatment of postmenopausal breast cancer patients with letrozole: A randomized double-blind multicenter study显示文摘 | W. Eiermann S. Paepke J. Appfelstaedt A. Llombart-Cussac J. Eremin J. Vinholes L. Mauriac M. Ellis M. Lassus H. A. Chaudri-Ross M. Dugan M. Borgs V. Semiglazov | 2001 | Annals of Oncology2001，，11： | 1 |
| 10 | Using cast electroslag blanks instead of forging in flange production显示文摘 | EREMIN E N ZHEREBTSOV S N GRITSEVICH V N | 2003 | Chemical and Petroleum Engineering2003，39，： | 1 |
| 11 | Comparative study of three immunoassays based on monoclonal antibodies for detection of the pesticide parathion-methyl in real samples 显示文摘 | Kolosova A Y Park J H Eremin S A | 2004 | Analytica Chimica Acta2004，511，： | 1 |
| 12 | A polarization fluorescence immunoassay for the herbicide 显示文摘 | Krasnova A I Eremin S A Natangelo M | 2001 | Analytical Letters2001，34，13： | 1 |
| 13 | Magnetic-field- and bias-sensitive conductivity of a hybrid Fe/Si02/p-Si structure in planar geometry 显示文摘 | VOLKOV N V TARASOV A S EREMIN E V VARNAKOV S N OVCHINNIKOV S G ZHARKOV S M | 2011 | J Appl Phys2011，109，12： | 1 |
| 14 | Predicting response to neoadjuvant chemotherapy in breast cancer: molecular imaging, systemic biomarkers and the cancer metabolome 显示文摘 | Chuthapisith S EreminJ M Eremin O | 2008 | Oncol Rep2008，20，4： | 1 |
| 15 | Preliminary screening method for dioxin contamination using polarization fluoroimmunoassay for chlorinated phenoxyacid pesticides 显示文摘 | Krikunova V S Eremin S A Smith D S | 2003 | Environ Anta Chem2003，83，7： | 1 |
| 16 | A mathematical model of microbial enhanced oil recovery method for mixed type rock 显示文摘 | Sitnikov A A Eremin N A | 2004 | SPE (S0016- 1844)2004，12，： | 1 |
| 17 | Evidence for Fas counter attack in vivo from a study of colorectal cancer显示文摘 | Arbuckle E Langlois NE Eremin O | 2000 | Oncol Rep2000，7，： | 1 |
| 18 | Developmental changes in the expression of low-voltage-activated Ca^2+ channels in rat visual cortical neurones显示文摘 | Tarasenko A N Isaev D S Eremin A V | 1998 | J Physiol1998，509，2： | 1 |
| 19 | The management of cancer of the breast in the elderly 显示文摘 | Ashkanani F Eremin O Heys SD | 2003 | Enr Surg Oncol2003，4，5： | 1 |
| 20 | Efremova on the Mechanism of Impurity Influence on Growth Kinetics and Surface Morphology of KDP Crystals- I :Defect Centers Formed by Bivalent and Trivalent Impurity Ions Incorporated in KDP Structure-theoretical Study显示文摘 | Miroslawa R Eremin N N Eremina T A | 2005 | Journal of Crystal Grow&2005，273，： | 1 |
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