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| 1 | Surface modification to improve dyeing of cotton fabric with a cationic dye显示文摘 | PISUNTORNSUG C YANUMET N EDGAR A O | 2002 | Coloration Technology2002,118,2: | 1 |
| 2 | Design robust nonlinear controllers for autonomous underwater vehicle with comparison simulated and at-sea test data显示文摘 | SONG Fei-jun Edgar A N SMITH S M | 2002 | Journal of vibration and control2002,,8: | 1 |
| 3 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022,28,29: | 1 |
| 4 | Olefin Oxidation with Dioxygen Catalyzed by Poq)hyrins and Phthalocyanines Intercalated in a - zirconium Phosphate 显示文摘 | Martha E N Sonia A G Edgar A P | 2001 | J Mol Catal A : Chem2001,,175: | 1 |
| 5 | Air infiltration measurements in a home using a convenient perfluorocarbon tracer techniqiue显示文摘 | RUSSELL N D EDGAR A C | 1982 | Environment International1982,,8: | 1 |
| 6 | CXCR4 physically associates with the T cell receptor to signal in T cells显示文摘 | Kumar A Humphreys T D Kremer K N Bramati P S Brad- field L Edgar C E | 2006 | Immunity2006,25,: | 1 |
| 7 | The in vitro detection of early enamel de-and re-mineralization adjacent to bonded orth- odontic cleats using quantitative light-induced fluorescence 显示文摘 | Preytt A Pender N Edgar WM | 2003 | Eur J Orthod2003,25,3: | 1 |
| 8 | Plasma membrane H^+-ATPase is involved in auxin-mediated cell elongation during wheat embryo development显示文摘 | NICOLE R K JOLANA T P A SONJA F NORBERT H WOLFGANG M EDGAR W VOLKER S GUNTHER N CHRISTIANE F I | 2003 | Plant Physiol2003,131,3: | 1 |
| 9 | Design robust nonlinear controllers for autonomous underwater vehicle with comparison simulated and at-sea test data显示文摘 | SONG Fei-jun Edgar A N SMITH S M | 2002 | Journal of vibration and control2002,,8: | 1 |
| 10 | Biases associated with the use of underwater visual census techniques to quantify the density and size-structure of fish populations显示文摘 | Edgar G J Barrett N S Morton A J | 2004 | Journal of Experimental Marine Biology and Ecology2004,308,2: | 1 |
| 11 | Using Acetone as Solvent to Study Removal of Anthracene in Soil Inhibits Microbial Activity and Alters Nitrogen Dynamics显示文摘 | Edgar Vázquez Nú?ez Viviana Rodríguez Alejandro García Gaytán Marco Luna-Guido Liliana A. Betancur-Galvis Rodolfo Marsch Luc Dendooven | 2009 | Archives of Environmental Contamination and Toxicology2009,,2: | 1 |
| 12 | Effect of cerium dioxide, titanium dioxide, silver, and gold nanoparticles on the activity of microbial communities intended in wastewater treatment显示文摘 | Ana García Lucía Delgado Josep A. Torà Eudald Casals Edgar González Víctor Puntes Xavier Font Julián Carrera Antoni Sánchez | 2011 | Journal of Hazardous Materials2011,,: | 1 |
| 13 | Arthrospira maxima(Spirulina)prevents endoplasmic reticulum stress in the kidney through its C-phycocyanin显示文摘Arthrospira maxima(Spirulina)is a cyanobacterium which is considered a nutraceutical because it has antioxidant,anti-inflammatory,and cytoprotective properties in different renal disease models(RodriguezSánchez et al.,2012;Aziz et al.,2018;Memije-Lazaro et al.,2018). | Placido ROJAS-FRANCO Margarita FRANCO-COLÍN Vanessa BLAS-VALDIVIA María Estela MELENDEZ-CAMARGO Edgar CANO-EUROPA | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021,22,7: | 0 |
| 14 | Development of a Construction Material for Indoor and Outdoor, Metakaolinite-Based Geopolymer, with Environmental Properties显示文摘Environmental problems that came from the human activity have many facets,since pollution of the atmosphere arises from vehicles,industrial emissions while pollution of water could be from organic compounds,pesticides etc.These can cause serious health effects,such as respiratory diseases,including asthma and lung cancer.Hence,in the present work,a kinetic study on the effective adsorption and photo degradation of methylene blue(MB)dye,under ultraviolet A(UVA)irradiation of an alkali activated inorganic polymer(geopolymer)with homogeneously dispersed titanium dioxide(TiO2)micro-particles is presented.In addition,antimicrobial testing of the alkali activated TiO2 material was performed showing a bacteriostatic effect. | M.Mondragón-Figueroa Héctor.R.Guzmán-Carrillo MiguelÁngel Rico JoséLuis.Reyez-Araiza Jorge.Pineda-Piñón Edgar J.López-Naranjo María C.Columba-Palomares Jóse.M.López-Romero Josè.Ramón Gasca-Tirado Alejandro.Manzano-Ramírez | 2019 | 材料科学与工程(中英文A版)2019,9,4: | 0 |