|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Efficient RT-QuIC seeding activity for α-synuclein in olfactory mucosa samples of patients with Parkinson’s disease and multiple system atrophy显示文摘Background:Parkinson’s disease(PD)is a neurodegenerative disorder whose diagnosis is often challenging because symptoms may overlap with neurodegenerative parkinsonisms.PD is characterized by intraneuronal accumulation of abnormalα-synuclein in brainstem while neurodegenerative parkinsonisms might be associated with accumulation of eitherα-synuclein,as in the case of Multiple System Atrophy(MSA)or tau,as in the case of Corticobasal Degeneration(CBD)and Progressive Supranuclear Palsy(PSP),in other disease-specific brain regions.Definite diagnosis of all these diseases can be formulated only neuropathologically by detection and localization ofα-synuclein or tau aggregates in the brain.Compelling evidence suggests that trace-amount of these proteins can appear in peripheral tissues,including receptor neurons of the olfactory mucosa(OM).Methods:We have set and standardized the experimental conditions to extend the ultrasensitive Real Time Quaking Induced Conversion(RT-QuIC)assay for OM analysis.In particular,by using human recombinantα-synuclein as substrate of reaction,we have assessed the ability of OM collected from patients with clinical diagnoses of PD and MSA to induceα-synuclein aggregation,and compared their seeding ability to that of OM samples collected from patients with clinical diagnoses of CBD and PSP.Results:Our results showed that a significant percentage of MSA and PD samples inducedα-synuclein aggregation with high efficiency,but also few samples of patients with the clinical diagnosis of CBD and PSP caused the same effect.Notably,the final RT-QuIC aggregates obtained from MSA and PD samples owned peculiar biochemical and morphological features potentially enabling their discrimination.Conclusions:Our study provide the proof-of-concept that olfactory mucosa samples collected from patients with PD and MSA possess important seeding activities forα-synuclein.Additional studies are required for(i)estimating sensitivity and specificity of the technique and for(ii)evaluating its application for the diagnosis of PD and neurodegenerative parkinsonisms.RT-QuIC analyses of OM and cerebrospinal fluid(CSF)can be combined with the aim of increasing the overall diagnostic accuracy of these diseases,especially in the early stages. | Chiara Maria Giulia De Luca Antonio Emanuele Elia Sara Maria Portaleone Federico Angelo Cazzaniga Martina Rossi Edoardo Bistaffa Elena De Cecco Joanna Narkiewicz Giulia Salzano Olga Carletta Luigi Romito Grazia Devigili Paola Soliveri Pietro Tiraboschi Giuseppe Legname Fabrizio Tagliavini Roberto Eleopra Giorgio Giaccone Fabio Moda | 2019 | Translational Neurodegeneration2019，8，1： | 5 |
| 2 | De-sign,synthesis and biological evaluation of novel stilbene-based antitumor agents显示文摘 | SIMONI D INVIDIATA F P ELEOPRA M | 2009 | Bioorg Med Chem2009，17，2： | 1 |
| 3 | The roal of gustatory flushing in Frey's syndrome and its treatment with botulinum toxin type A显示文摘 | Tugnoli V Marchese Ragona R Eleopra R | 2002 | Clin Auton Res2002，12，3： | 1 |
| 4 | The role of gustatory flushing in Frey's syndrome and its treatment with botulinum toxin type A显示文摘 | Tugnoli V Marchese RR Eleopra R | 2002 | Clin Auton Res2002，12，3： | 1 |
| 5 | The role of gustatory flushing in Frey's syndrome and its treatment with botulinum toxin type A显示文摘 | Tugnoli Marchese RR Eleopra R | 2002 | Clin Auton Res2002，12，3： | 1 |
| 6 | The role of gustatory flushing in Frey syndrome and its treatment with botulinum toxin type A 显示文摘 | Tugnoli V Marchese Ragona R Eleopra R | 2002 | Clin Aut Res2002，12，3： | 1 |
| 7 | The role of gusta- tory flushing in Frey's syndrome and its treatment with botulinum toxin type A显示文摘 | Tugnoli V Marchese Ragona R Eleopra R | 2002 | Clin Auton Res2002，12，3： | 1 |
| 8 | The role of gustatory flushing in Frey's syndrome and its treatment with botulinum toxin type A显示文摘 | Tugnoli V Marchese Ragona R Eleopra R | | 0，，03： | 1 |
| 9 | The role of gustatory flushing in Frey' s syndrome and its treatment with botulinum toxin A 显示文摘 | Tugnoli V Marchese Ragona R Eleopra R | 2002 | Clin Auton Res2002，12，3： | 1 |
| 10 | Clinical use of non-A botulinum toxins: botulinum toxin type C and botulinum toxin type F显示文摘 | Eleopra R Tugnoli V Quatrale R | 2006 | Neurotox Res2006，9，23： | 1 |
| 11 | The role of gustatory flushing in Frey's syndrome and its treatment with botulinum toxin type A显示文摘 | Tusnoli V Marchese Ragona R Eleopra R | 2002 | Clin Auton Bes2002，12，3： | 1 |
| 12 | The role of gustatory flushing in Frey's syndrome and its treatment with botulinum toxin type A显示文摘 | Tugnoli V Marchese R Eleopra R | 2002 | Clin Auton Res2002，12，3： | 1 |
| 13 | Clinical use of non-A botulinum toxins : bot- ulinum toxin type B 显示文摘 | Dressler D Eleopra R | 2006 | Neurotox Res2006，9，23： | 1 |
| 14 | The role of gustatory flushing in Frey’s syndrome and its treatment with botulinum toxin type A显示文摘 | V. Tugnoli R. Marchese Ragona R. Eleopra R. Quatrale J. G. Capone A. Pastore C. Montecucco D. De Grandis | 2002 | Clinical Autonomic Research2002，，3： | 1 |
| 15 | The role of gustatory flushing in Frey's syndrome and its treatment with hotulinum toxin type A 显示文摘 | Tugnoli V Marchese Ragona R Eleopra R | 2002 | Clin Auton Res2002，12，3： | 1 |
| 16 | The role of gustatory flushing in Frey's syndrome and its treatment with botulinum toxin type A显示文摘 | Tugnoli V Marchese Ragona R Eleopra R | 2002 | Clin Auton Res2002，12，3： | 1 |
| 17 | Botulinum toxin treatment in the facial muscles of humans:evidence of an action in untreated near muscles by peripheral local diffusion显示文摘 | Eleopra R Tugnoli V Caniatti L De Grandis D | 1996 | Neurology1996，46，： | 1 |
| 18 | Different types of botulinum toxin in humans 显示文摘 | Eleopra R Tugnoli V Quatrale R | 2004 | Mov Disord2004，19，8： | 1 |
| 19 | Different time courses of recovery after poisoning with botulinum neurotoxin serotypes A and E in humans 显示文摘 | Eleopra R Tugnoli V Rossetto O | 1998 | Neurosci Lett1998，256，3： | 1 |
| 20 | Botulinum toxin type A and C effect on motor unit in humans: An electrophysiological study by motor mumber estimate显示文摘 | Eleopra R Tugnoli V Bombardi R | 1997 | Electroencephalogr Clin Neurophysiol1997，103，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员