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2篇 您的检索式：作者名="Ellen de Waal"

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		题名	作者	年代	出处	被引量
	1	Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA显示文摘	Muyzer G Ellen De Waal	1993	Appl Environ Microbial1993，59，3：	1
	2	Expression,purification and characterization of the soluble Cu_A domain of cytochrome c oxidase of Paracoccus versutus显示文摘The key subunit II of cytochrome c oxidase (CcO) contains a soluble binuclear copper center (CuA) domain. The CUA domain of Paracoccus versutus was cloned, expressed, purified and characterized. The gene encoding the CUA domain in pETlld vector was expressed in E. coli BL21 (DE3). The results showed that the CuA domain was expressed mostly in inclusion bodies and the CUA domain protein synthesized in E. coli cells represents approximately 10 percent of the total cellular proteins. Dissolved in urea, dia-lyzed and recombined with Cu+/Cu2+ and purified by the Q-sepharose fast flow anion-exchange column and Sephadex G-75 gel filtration column, the soluble purple-colored protein, which shows a single band in electrophoresis, was obtained. The UV-visible absorption spectrum of CUA domain showed that there are intense band at 478 nm and a shoulder peak at 530 nm, and two weak bands at 360 and 806 nm respectively, which can be assigned to the charge transfer and the interactions of obitals of Cu-S and Cu-Cu	LI Lianzhi SONG Aixin XIE Yi HUANG Zhongxian Ellen de Waal Urszula Kolczak Gerard W.Canters	2001	Chinese Science Bulletin2001，46，19：	0

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