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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Ischemic brain injury is mediated by the activation of poly (ADP- ribose)polymerase显示文摘 | ENDRES M Wang ZQ Namura S | | 0，，： | 2 |
| 3 | Attenuation of delayed neuronal death after mild focal ischemia in mice by inhibition of the caspase family显示文摘 | Namum S Shimizu-Saaamata M | 1998 | J Cereb Blood Flow Metab1998，18，3： | 1 |
| 4 | (n-3)fatty acids supplementation suppresses cytokine production and lymphocyte proliferation:Comparison between young and older women显示文摘 | Meydani S Endres S Woods M | 1997 | Nutr1997，121，： | 1 |
| 5 | Afro-assisted investigation of the corrosion behaviour of magnesium and Az91 alloys in an ionic liquid with varying water content显示文摘 | Shkurankov A E1 Abedin S Z Endres F | 2007 | Australian Journal of Chemistry2007，60，1： | 1 |
| 6 | Ischemic brain injury is mediated by the activation of poly(ADP-ribose) polymerase显示文摘 | Endres M Wang ZQ Namura S | 1997 | J Cereb Blood Flow Metab1997，17，11： | 1 |
| 7 | Risk factorsfor infection after spinal surgery 显示文摘 | Fang h Hu S Endres N | 2005 | Spine2005，30，12： | 1 |
| 8 | Efficacy of tranexamic acid in reducing blood loss in posterior lumbar spine surgery for degenerative spinal stenosis with instability: a retrospective case control study 显示文摘 | Endres S Heinz M Wilke A | 2011 | BMC Surg2011，11，： | 1 |
| 9 | Risk factors for infection after spinal sugery显示文摘 | Fang A Hu S S Endres N | 2005 | Spine2005，30，12： | 1 |
| 10 | Instrumented intervertebral or pos- terolateral fusion in elderly patients clinical results of a single cen-ter 显示文摘 | Endres S Aigner R Wilke A | 2011 | B MC Musculoskelet Disord2011，12，1： | 1 |
| 11 | A 3D environ-ment for anulus fibrosus regeneration 显示文摘 | Cabraja M Endres M Vetterlein S | 2012 | Neurosurg Spine2012，17，2： | 1 |
| 12 | Elevated cell-associated levels of interleukin 1ββ and inter-leukin 6 in inflamed mucosa of inflammatory bowel disease 显示文摘 | Grottrup-Wolfers E Moeller J Karbaeh U Muller-Lissner S Endres S | 1996 | Eur J Clin Invest1996，26，2： | 1 |
| 13 | Ischemic brain injury is mediated by the activation of poly(ADP-ribose) polymerase显示文摘 | Endres M Wang ZQ Namura S | 1997 | J Cereb Bolld Flow Metab1997，17，11： | 1 |
| 14 | A study on the electrodeposition of tantalum on NiTi alloy in an ionic liquid and corrosion behaviour of the coated alloy显示文摘 | Zein El Abedin S Welz-Biermann U Endres F | 2005 | Electrochemistry Communications2005，7，9： | 1 |
| 15 | Inhibition of Toll-like receptor 7- and 9- mediated alpha/beta interferon production in human plasmacytoid dendritic cells by respiratory syncytial virus and measles virus 显示文摘 | Schlender J Hornung V Finke S Gfinthner-Biller M Marozin S Brz6zka K Moghim S Endres S Hartmann G Conzelmann KK | 2005 | J Virol2005，79，9： | 1 |
| 16 | Risk factors for infection after spinal surgery显示文摘 | FANG A HU S ENDRES N | 2005 | Spine2005，30，12： | 1 |
| 17 | Ischemic brain injury is mediated by the activation of poly(ADP-ribose)polymerase显示文摘 | Endres m Wang ZQ Namura S Waeber C | 1997 | J Cereb Blood Flow Metab1997，17，11： | 1 |
| 18 | Catalytical conversion of carbohydrates in subcritical water: A new chemical process for lactic acid production显示文摘 | Bicker M Endres S Ott L | 2005 | Journal of Molecular Catalysis A: Chemical2005，239，12： | 1 |
| 19 | NO-Donors,part 3:nitrooxyacylated thiosalicylates and salicylates-synthesis and biological activities显示文摘 | Endres S Hacker A Noack E | 1999 | Eur J Med Chem1999，34，11： | 1 |
| 20 | Bilipro- rein chromophore attachment, chaperone-like :unction of the PecE subunit of alpha-phycoeryth- phcyanin lyase 显示文摘 | Bohm S Endres S Scheer H Zhao K H | 2007 | J Biol Chem2007，282，25： | 1 |
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