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您的检索式：作者名="Engui Zhao"
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| 1 | An Aggregation-Induced Emission Optical Highlighter for the Studies of Endoplasmic Reticulum-Lipid Droplet Content Dynamics显示文摘The lipid droplet(LD)is a dynamic organelle responsible for lipid storage and metabolism that plays important roles in maintaining lipid homeostasis.However,limited strategies are available for tracking the LD content exchange.In this contribution,we report a novel fluorescent probe,TPEAmAl,for real-time LD content dynamics tracking.TPE-AmAl is LD-specific,but emits faintly due to its intramolecular motion.Upon photoactivation,it undergoes a photocyclodehydrogenation reaction and shows a large fluorescence increment.Thus,it can be used for highlighting selected LDs with high spatial resolution.By measuring the fluorescence changes in the distal region,the lipid content exchange efficiency can be estimated.In our experiment,LD content exchange rate differences between nascent and mature LDs as well as cells with normal and deficient LD budding machinery are observed.This probe expands the fluorescence-based toolbox for LD content dynamics studies. | Engui Zhao Chuen Kam Ming-Yu Wu Erjing Wang Jiawei Wang Ben Zhong Tang Sijie Chen | 2022 | CCS Chemistry2022，4，2： | 0 |
| 2 | An AIE-active probe for efficient detection and high-throughput identification of outer membrane vesicles显示文摘Rapid detection and quantification of outer membrane vesicle(OMV)are of both scientific value and clinical implications.However,limited tools are available for investigations of OMVs.Herein,we report a novel fluorescent probe with aggregation-induced emission(AIE)characteristics,namely,OEO-TPE-MEM(OTM),for OMV detection.OTM emits faintly in an aqueous medium,but its fluorescence could be effectively turned on upon interacting with bacteria bodies and OMVs produced by Gram-negative bacteria.Notably,OTM could provide quantitative information on bacterial membrane remodeling and OMV secretion and be applied to high-throughput screening of OMV-inducing agents.This study presents a powerful AIE probe for imaging and quantitative analysis of bacteria envelop and derived OMVs,which might be applied for evaluating research and clinical antimicrobial materials in future studies. | Fei Wang Po-Yu Ho Chuen Kam Quanzhi Yang Jinzhao Liu Weiping Wang Engui Zhao Sijie Chen | 2023 | Aggregate2023，4，4： | 0 |
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