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| 1 | Functional expression of a proliferation-related ligand in hepatocellular carcinoma and its implications for neovascularization显示文摘AIM: To detect the expression of a proliferation-related ligand on human hepatocellular carcinoma (HCC) cell lines (SK-Hep1, HLE and HepG2) and in culture medium.METHODS: APRIL expression was analyzed by Western blotting in HCC cell lines. Effects of APRIL to cell count and angiogenesis were analyzed, too.RESULTS: Recombinant human APRIL (rhAPRIL) increased cell viability of HepG2 cells and, in HUVEC, rhAPRIL provided slight tolerance to cell death from serum starvation. Soluble APRIL (sAPRIL) from HLE cells increased after serum starvation, but did not change in SK-Hep1 or HepG2 cells. These cells showed down-regulation of VEGF after incubation with anti-APRIL antibody.Furthermore, culture medium from the HCC cells treated with anti-APRIL antibody treatment inhibited tube formation of HUVECs.CONCLUSION: Functional expression of APRIL might contribute to neovascularization via an upregulation of VEGF in HCC. | Hiroshi Okano Katsuya Shiraki Yutaka Yamanaka Hidekazu Inoue Tomoyuki Kawakita Yukiko Saitou Yumi Yamaguchi Naoyuki Enokimura Keiichi Ito Norihiko Yamamoto Kazushi Sugimoto Kazumoto Murata Takeshi Nakano | 2005 | World Journal of Gastroenterology2005，11，30： | 13 |
| 2 | Augmentation of tumor necrosis factor family-induced apoptosis by E3330 in human hepatocellular carcinoma cell lines via inhibition of NFκB显示文摘AIM: To investigate the reduction of cell viability in human hepatocellular carcinoma (HCC) cell lines induced by inhibition of nuclear factor κB (NFκB). METHODS: HLE, SKHep1, and HepG2 were incubated and E3330 was used to compare the stimulation of some chemotherapeutic drugs with that of TNF family, Fas ligand, TNFα and TNF-related apoptosis-inducing ligand (TRAIL) at the point of the reduction of cell viability by inhibiting NFκB. RESULTS: E3330 decreased NFκB levels in HLE cells stimulated by TNF and TRAIL. The cytotoxicity of the combination of TRAIL, TNFα, Fas ligand, and E3330 increased synergistically in a dose-dependent manner compared to either E3330 alone in all HCC cell lines by MTT assay. However, the combination of some chemotherapeutic drugs and E3330 did not decrease the cell viability. CONCLUSION: Inhibition of NFκB sensitizes human HCC cell lines to TNF-mediated apoptosis including TRAIL, and TRAIL-based tumor therapy might be a powerful potential therapeutic tool in the treatment of human HCC. | Yukiko Saitou Katsuya Shiraki Takenari Yamanaka Kazumi Miyashita Tomoko Inoue Yutaka Yamanaka Yumi Yamaguchi Naoyuki Enokimura Norihiko Yamamoto Keiichi Itou Kazushi Sugimoto Takeshi Nakano | 2005 | World Journal of Gastroenterology2005，11，40： | 3 |
| 3 | Adenoviral-mediated transfer of p53 gene enhances TRAIL-induced apoptosisin human hepatocellular carcinoma cells显示文摘 | Hidekazu Inoue Katsuya Shiraki Kazumoto Murata Kazushi Sugimoto Tomoyuki Kawakita Yumi Yamaguchi Yukiko Saitou Naoyuki Enokimura Norihiko Yamamoto Yutaka Yamanaka Takeshi Nakano | 2004 | International Journal of Molecular Medicine2004，，2： | 1 |
| 4 | Vitamin K analog (compound 5) induces apoptosis in human hepatocellular carcinoma independent of the caspase pathway显示文摘 | Enokimura N Shiraki K Kawakita T | 2005 | Anticancer Drugs2005，16，8： | 1 |
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