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| 1 | Multispecies probiotic protects gut barrier function in experimental models显示文摘AIM:To investigate the effect of the probiotic combination Lactibiane Tolerance?(LT)on epithelial barrier function in vitro and in vivo.METHODS:The effect of the multispecies probiotic LT was assessed on several models of epithelial barrier function both in vitro(in basal and inflammatory conditions)and in vivo[visceral hypersensitivity induced by chronic stress or by colonic perfusion of a fecal supernatant(FSN)from patients with irritable bowel syndrome(IBS)].In vitro,we measured the permeability of confluent T84 cell monolayers incubated with or without LT by evaluating the paracellular flux of macromolecules,in basal conditions and after stimulation with lipopolysaccharide(LPS)or with conditioned medium of colonic biopsies from IBS patients(IBS-CM).In vivo,male C57/Bl6 mice received orally NaCl or LT for 15 d and were submitted to water avoidance stress(WAS)before evaluating visceral sensitivity by measuring the myoelectrical activity of the abdominal muscle and the paracellular permeability with 51Cr-EDTA.Permeability and sensitivity were also measured after colonic instillation of FSN.Tight-junctions were assessed by immunoblotting and TLR-4 expression was evaluated by immunohistochemistry RESULTS:Incubation of T84 cell monolayers with LT in basal conditions had no significant effect on permeability(P>0.05 vs culture medium).By contrast,addition of LT bacterial bodies(LT)completely prevented the LPS-induced increase in paracellular permeability(P<0.01 vs LPS 10 ng/mL(LPS 10);P<0.01 vs LPS 100ng/mL(LPS 100),P>0.05 vs culture medium).The effect was dose dependent as addition of 109 LT bacterial bodies induced a stronger decrease in absorbance than 106 LT(109 LT+LPS 10:-20.1%±13.4,P<0.01vs LPS 10;106 LT+LPS 10:-11.6%±6.2,P<0.01 vs LPS 10;109 LT+LPS 100:-14.4%±5.5,P<0.01 vs LPS 100;106 LT+LPS 100:-11.6%±7.3,P<0.05 vs LPS 100).Moreover,the increase in paracellular permeability induced by culturing T84 cells with conditioned medium of colonic biopsies from IBS patients(IBS-CM)was completely inhibited in the presence of 109 LT(P<0.01 vs IBS-CM).LT also significantly prevented the epithelial disruption induced by intracolonic infusion of fecal supernatant from IBS patients(P<0.01 vs IBS FSN)or water avoidance stress P<0.01 vs WAS)in C57/Bl6 mice and increased the expression of occludin in vitro and in vivo,as assessed by immnunoblotting.The WAS-induced effect on visceral sensitivity was prevented by LT treatment since values obtained for all steps of colorectal distension were significantly(P<0.01)different from the WAS group.Finally,LT downregulated the response mediated through TLR-4 in vitro(decrease in tumor necrosis factorαsecretion in response to LPS:-65.8%for 109 LT and-52.5%for 106LT,P<0.01 vs LPS)and in vivo(inhibition of WAS induced an increase in TLR-4 expression in the LT treated mice colon,P<0.01 vs WAS).CONCLUSION:The probiotic LT mix prevented the disruption to the epithelial barrier induced by LPS,stress or colonic soluble factors from IBS patients and prevented visceral hypersensitivity. | Mylene Nébot-Vivinus Cherryl Harkat Hanene Bzioueche Christel Cartier Raffaella Plichon-Dainese Lara Moussa Helene Eutamene Dorsa Pishvaie Sophie Holowacz Christian Seyrig Thierry Piche Vassilia Theodorou | 2014 | World Journal of Gastroenterology2014，20，22： | 12 |
| 2 | Oral treatment with plecanatide or dolcanatide attenuates visceral hypersensitivity via activation of guanylate cyclase-C in rat models显示文摘AIM To investigate the effects of plecanatide and dolcanatide on maintenance of paracellular permeability, integrity of tight junctions and on suppression of visceral hypersensitivity. METHODS Transport of fluorescein isothiocyanate(FITC)-dextran was measured to assess permeability across cell monolayers and rat colon tissues. Effects of plecanatide and dolcanatide on the integrity of tight junctions in Caco-2 and T84 monolayers and on the expression and localization of occludin and zonula occludens-1(ZO-1) were examined by immunofluorescence microscopy. Anti-nociceptive activity of these agonists was evaluated in trinitrobenzene sulfonic acid(TNBS)-induced inflammatory as well as in non-inflammatory partial restraint stress(PRS) rat models. Statistical significance between the treatment groups in the permeability studies were evaluated using unpaired t-tests.RESULTS Treatment of T84 and Caco-2 monolayers with lipopolysaccharide(LPS) rapidly increased permeability, which was effectively suppressed when monolayers were also treated with plecanatide or dolcanatide. Similarly, when T84 and Caco-2 monolayers were treated with LPS, cell surface localization of tight junction proteins occludin and ZO-1 was severely disrupted. When cell monolayers were treated with LPS in the presence of plecanatide or dolcanatide, occludin and ZO-1 were localized at the cell surface of adjoining cells, similar to that observed for vehicle treated cells. Treatment of cell monolayers with plecanatide or dolcanatide without LPS did not alter permeability, integrity of tight junctions and cell surface localization of either of the tight junction proteins. In rat visceral hypersensitivity models, both agonists suppressed the TNBS-induced increase in abdominal contractions in response to colorectal distension without affecting the colonic wall elasticity, and both agonists also reduced colonic hypersensitivity in the PRS model. CONCLUSION Our results suggest that activation of GC-C signaling might be involved in maintenance of barrier function, possibly through regulating normal localization of tight junction proteins. Consistent with these findings, plecanatide and dolcanatide showed potent antinociceptive activity in rat visceral hypersensitivity models. These results imply that activation of GC-C signaling may be an attractive therapeutic approach to treat functional constipation disorders and inflammatory gastrointestinal conditions. | Illona-Marie Boulete Anusha Thadi Catherine Beaufrand Viren Patwa Apoorva Joshi John A Foss E Priya Eddy Helene Eutamene Vaseem A Palejwala Vassilia Theodorou Kunwar Shailubhai | 2018 | World Journal of Gastroenterology2018，24，17： | 5 |
| 3 | Prevention of gut leakiness by a probiotic treatment leads to attenuated HPA response to an acute psychological stress in rats显示文摘 | Afifa Ait-Belgnaoui Henri Durand Christel Cartier Gilles Chaumaz Hélène Eutamene Laurent Ferrier Eric Houdeau Jean Fioramonti Lionel Bueno Vassilia Theodorou | 2012 | Psychoneuroendocrinology2012，，11： | 2 |
| 4 | Mannodendrimers prevent acute lung inflammation by inhibiting neutrophil recruit?ment显示文摘 | Blattes E Vercellone A Eutamene H | 2013 | Proc Natl Acad Sci USA2013，110，28： | 1 |
| 5 | LPS-induced lung inflammation is linked to increased epithelial permeability:role of MLCK显示文摘 | Eutamene H Theodorou V Schmidlin F | 2005 | Eur Respir J2005，25，5： | 1 |
| 6 | Synergy between Laetobaeillus parocasei and its bacteri- al products to counteract stress-induced gut permeability and sensitivity increase in rats显示文摘 | Helene Eutamene Florence Lamine Chantal Chabo | 2007 | The Journal of Nutrition2007，137，8： | 1 |
| 7 | Stressinduced visceral hypersensitivity to rectal distension in rats:role of CRF and mast cells显示文摘 | Gue M Del Rio-Lacheze C Eutamene H | 1997 | Neuro gastroenterol Motil1997，9，4： | 1 |
| 8 | Synergy between Lactobacillus paracasei and its bacterial products to counteract stress-induced gut permeability and sensitivity increase in rats显示文摘 | Eutamene H Lamine F Chabo C | 2007 | J Nutr2007，137，8： | 1 |
| 9 | Lactobaeillus far- ciminis treatment attenuates stress-induced overexpression of fos protein in spinal and supraspinal sites after colorectal distension in rats显示文摘 | Ait-Belgnaoui A Eutamene H Houdeau E | 2009 | Neurogastroenterol Motil2009，21，5： | 1 |
| 10 | Fecal proteases from diarrheic-IBS and ulcerative colitis patients exert opposite effect on visceral sensitivity in mice显示文摘 | Anita Annaházi Krisztina Gecse Marta Dabek Afifa Ait-Belgnaoui András Rosztóczy Richárd Róka Tamás Molnár Vassilia Theodorou Tibor Wittmann Lionel Bueno Helene Eutamene | 2009 | Pain2009，，1： | 1 |
| 11 | Guanylate cyclase C - me- diated antinociceptive effects of linaelotide in rodent models of visceral pain显示文摘 | Eutamene H Bradesi S Larauche M | 2010 | Neurogastroenterol Moti12010，22，3： | 1 |
| 12 | Acute and chronic stress differently affect visceral sensitivity to rectal distension in female rats显示文摘 | Bradesi S Eutamene H Garcia-Villar R | 2002 | Neurogastroenterol Motil2002，14，1： | 1 |
| 13 | Guanylate cyclase C-mediated antinociceptive effects of linaclotide in rodent models of visceral pain显示文摘 | EUTAMENE H BRADESI S LARAUCHE M | 2010 | Neurogastroenterol Motil2010，22，3： | 1 |
| 14 | Lactobacillus farciminis treatment attenuates stress induced over-expression of c-fos protein in spinal and supraspinal sites after colorectal distension in rats 显示文摘 | Ait-Belgnaoui A Eutamene H Houdeau E | 2009 | Neurogastroenterol Moti12009，21，5： | 1 |
| 15 | Stress induced visceral hypersensitivity to rectal distension in rats:role of CRF andmas tcells 显示文摘 | Gue M Lacheze C Eutamene H | 1997 | Neurogastroenterol Motil1997，9，： | 1 |
| 16 | Evidence of central and pe ripheral sensitization in a rat model of narcotic bowel-like syn- drome显示文摘 | Aqostini S Eutamene H Cartier C | 2010 | Gastroenterology2010，139，2： | 1 |
| 17 | Preparation and Aqueous Properties of Starch--grafted Polyacrylamide Copolymers显示文摘 | Mohamed Eutamene Abbes Benbakhti Mohamed Khodja Amane Jada | 2009 | Starch2009，61，： | 1 |
| 18 | Preparation and aqueous properties of starch-grafted polyacrylamide copolymers 显示文摘 | Eutamene M Benbakhti A Khodja M | 2009 | Starch-Starke2009，，2： | 1 |
| 19 | LPS - induced lunginflammation is linked to increased epithelial permeability : role ofMLCK显示文摘 | Eutamene H Theodorou V Schmidlin F | 2005 | Eur Respir J2005，25，5： | 1 |
| 20 | Role of probioties in correcting abnormalities of colonic flora induced by stress显示文摘 | Eutamene H Bueno L | 2007 | Gut2007，56，： | 1 |
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