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| 1 | 用ALV-Jgp85单克隆抗体证明蛋鸡存在J亚群禽白血病显示文摘采用免疫组化法,对病理学初步诊断为蛋用型鸡 J亚群白血病的自然发病鸡的肿瘤、骨髓、肝脏、脾脏、肾脏、肺脏、心脏、胰脏、输卵管、卵巢、腺胃、骨骼肌、大脑、坐骨神经,用特异性抗 J亚群禽白血病病毒(ALV J)囊膜糖蛋白gp85的单克隆抗体进行检测,待检的组织切片中均检出阳性抗原,免疫组化的研究结果与病理学诊断结果相一致。在国内外首次发现并报道蛋用型鸡 J亚群禽白血病的自然病例。 | 徐镔蕊 董卫星 余春明 冯小宇 李宁 Lucy F.Lee Maoxiang Li | 2005 | 畜牧兽医学报2005，36，3： | 52 |
| 2 | 蛋鸡J亚群禽白血病的分子生物学诊断显示文摘根据J亚群白血病病毒(ALV-J)原型株HPRS-103的序列设计了一对针对外源性ALV-J引物H5和H7,从发生ML病死鸡的肿瘤、骨髓、肝脏、脾脏和输卵管组织中提取DNA作为模板,经PCR扩增得到长度为545bp的片段,对其序列进行测定后,与ALV-J原型株HPRS-103的序列进行了比较,发现其核苷酸同源性为97.4%,所编码氨基酸的同源性为96.1%。该片段含有ALV-Jgp85编码基因的部分序列和ALV-Jpol基因的部分序列,从分子水平上证实了蛋鸡发生J亚群禽白血病,进一步证明了此前根据病理学观察、免疫组化及免疫荧光诊断的结果。这是首次从分子水平上证明蛋用型鸡发生J亚群禽白血病。 | 徐镔蕊 董卫星 余春明 Lucy F.Lee Maoxiang Li | 2005 | 病毒学报2005，21，4： | 39 |
| 3 | Huanglongbing:An overview of a complex pathosystem ravaging the world's citrus显示文摘Citrus huanglongbing(HLB) has become a major disease and limiting factor of production in citrus areas that have become infected. The destruction to the affected citrus industries has resulted in a tremendous increase to support research that in return has resulted in significant information on both applied and basic knowledge concerning this important disease to the global citrus industry. Recent research indicates the relationship between citrus and the causal agent of HLB is shaped by multiple elements, in which host defense responses may also play an important role. This review is intended to provide an overview of the importance of HLB to a wider audience of plant biologists. Recent advances on host-pathogen interactions, population genetics and vectoring of the causal agent are discussed. | John V.da Graca Greg W.Douhan Susan E.Halbert Manjunath L.Keremane Richard F.Lee Georgios Vidalakis Hongwei Zhao | 2016 | Journal of Integrative Plant Biology2016，58，4： | 15 |
| 4 | 蛋鸡J亚群禽白血病病毒的分离与初步鉴定显示文摘 | 冯小宇 徐镔蕊 Lucy F.Lee Maoxiang Li | 2008 | 中国兽医杂志2008，44，1： | 14 |
| 5 | Climate change and plague history in Europe显示文摘Previous research that reported the linkage between climate change and plague activity primarily refers to the immediate effect of short-term climatic variation. Yet, decades of discussion about the climate-plague association cannot determine the precise role of climate in shaping plague dynamics. One reason for this discrepancy originates from the narrow selection of spatio-temporal settings for comprehensive analysis of the correlation, leading to a limited consideration of the complexity of possible dynamics.By analyzing a 414-year long record of plague outbreak in pre-industrial Europe and the corresponding climatic data in multi-scale,we find little evidence to support climate-plague correlation in(1) both climatic variations and large-scale climatic phenomena,(2)both country scale and continental scale,(3) annual to inter-annual scale, and(4) both linear and non-linear analytic approaches. The null-result should not be viewed as a general rejection of other recent findings related to climate-plague association; nevertheless,it suggests that a wider consideration of scales, sensitivity checks and consideration of contexts should be included in explaining and predicting plague transmission under contemporary global climate conditions. | Ricci P.H.YUE Harry F.LEE | 2018 | Science China Earth Sciences2018，61，2： | 8 |
| 6 | 利用重组反转录病毒表达马立克氏病病毒Meq基因显示文摘为研究马立克氏病病毒(MDV)Meq蛋白的生物学功能,应用磷酸钙与MDVMeq基因重组的反转录病毒转染载体质粒RCAS-Meq的DNA,转染于鸡胚成纤维细胞系DF1。经间接免疫荧光技术研究表明,转染后第3天即开始有少量DF1细胞在细胞核表达Meq蛋白,以后阳性细胞增多,到转染后第7天阳性细胞最多,之后荧光即衰减;通过酶联免疫吸附试验检测,发现细胞培养上清中游离病毒在转染后第5天即可检出,直至第7天仍维持最高水平,第9天开始下降。用细胞培养上清感染DF1细胞单层,于感染后第2天即可检出Meq蛋白阳性细胞,之后阳性细胞逐渐增多,到第4~5天达高峰,之后逐渐衰减;培养上清中的游离病毒在感染后第4天即可被检出,第5~7天达最高值。研究结果表明:(1)磷酸钙法能很好地将重组质粒RCAS-MeqDNA转染到DF1细胞中,而对细胞的损伤很小;(2)转染后细胞释放出的重组病毒能感染DF1细胞并稳定地表达Meq蛋白。表明Meq基因在构建的重组病毒基因组中的存在及其转录表达都是稳定的。这为在体内和体外研究MDVMeq基因的生物学功能提供了一种非常有效的方法和途径;(3)Meq蛋白仅分布于细胞核(包括核质和核仁)。这一结果证实了? | 韦平 Luck F.Lee | 1999 | 中国兽医学报1999，19，2： | 7 |
| 7 | Two centuries of April-July temperature change in southeastern China and its influence on grain productivity显示文摘China is a traditional agriculture based country and one main region for crop production is southeastern China where temperature is a dominant climate variable affecting agriculture. Temperature and social disturbances both influence crop production, yet distinguishing their relative impacts is difficult due to a lack of reliable, high-resolution historical climatic records before the very recent period. Here we present the first tree-ring based warm-season temperature reconstruction for southeastern China, a core region of the East Asian monsoon, for the past 227 years. The reconstruction target was April-July mean temperature, and our model explained 60.6% of the observed temperature variance during 1953–2012.Spatial correlation analysis showed that the reconstruction is representative of April-July temperature change over most of eastern China. The reconstructed temperature series agrees well with China-scale(heavily weighted in eastern China) agricultural production index values quite well at decadal timescales.The impacts of social upheavals on food production, such as those in the period 1920–1949, were confirmed after climatic influences were excluded. Our study should help distinguish the influence of social disturbance and warm-season temperature on grain productivity in the core agricultural region of China during the past two centuries. | Jiangfeng Shi Jinbao Li David D.Zhang Jingyun Zheng Shiyuan Shi Quansheng Ge Harry F.Lee Yesi Zhao Jie Zhang Huayu Lu | 2017 | Science Bulletin2017，62，1： | 6 |
| 8 | 原位PCR和原位杂交检测蛋鸡J亚群禽白血病病毒显示文摘根据ALV_J原型株HPRS10 3株gp85基因的内部序列 ,和pol基因的 3′端设计一对引物H5 H7。从发生ML病死蛋用型鸡的肿瘤、骨髓、肝脏、脾脏和输卵管组织中提取总RNA ,反转录为cDNA ,经PCR扩增得到长度为 5 4 5bp的ALV_JcDNA特异性探针。探针定位于 5 2 5 8~ 5 80 2bp。将病鸡的组织石蜡切片置HybaidExpress原位PCR仪平台上 ,以H5 H7为引物进行原位PCR扩增。应用地高辛标记的cDNA探针对原位PCR扩增后切片进行了原位杂交检测。结果在待检组织肿瘤组织、十二指肠、骨髓中出现明显的阳性信号。睾丸、肺、胰腺、大脑、输卵管、肾脏均检出散在的阳性信号。这是国内外首次从分子水平证明蛋鸡J亚群禽白血病。 | 徐镔蕊 乔素兰 董卫星 Lucy F.Lee LI Mao-xiang 秦玉明 李宁 | 2004 | 微生物学报2004，44，5： | 6 |
| 9 | Quantitative analysis of the impact of droughts and floods on internal wars in China over the last 500 years显示文摘Although many large-N quantitative studies have evidenced the adverse effects of climatic extremes on social stability in China during the historical period, most of them rely on temperature and precipitation as major explanatory variables, while the influence of floods and droughts on social crises is rarely measured. Furthermore, a comparison of the climate-society nexus among different geographic regions and at different temporal scales is missing in those studies. To address this knowledge gap,this study examines quantitatively the influence of floods and droughts on internal wars in three agro-ecological(rice, wheat,and pastoral) regions in China in AD1470–1911. Poisson regression and wavelet transform coherence analyses are applied to allow for the non-linear and non-stationary nature of the climate-war nexus. Results show that floods and droughts are significant in driving internal wars in historical China, but are characterized by strong regional variation. In the rice region, floods trigger internal wars at the inter-annual and multi-decadal time scales. In the wheat region, both floods and droughts cause internal wars at the inter-annual and multi-decadal time scales. In the pastoral region, internal wars are associated with floods only at the multi-decadal time scale. In addition, the multi-decadal coherence between hydro-climatic extremes and internal wars in all three of the agro-ecological regions is only significant in periods in which population density is increasing or the upper limit of regional carrying capacity is being reached. The above results imply that the climate-war nexus is mediated by regional geographic factors such as physical environmental setting and population pressure. Hence, we encourage researchers who study the historical human-climate relationship to boil down data according to geographic regions in the course of statistical analysis and to examine each region individually in follow-up studies. | Harry F.LEE David D.ZHANG PEI Qing JIA Xin Ricci P.H.YUE | 2017 | Science China Earth Sciences2017，60，12： | 3 |
| 10 | Differentiations of geographic distribution and subsistence strategies between Tibetan and other major ethnic groups are determined by the physical environment in Hehuang Valley显示文摘The people of the Tibetan Plateau have received extensive attention from scholars because of their unique adaptability to the low temperature and anoxic environments. However, the Tibetan communities and their habitats in the low-altitude regions of the plateau have rarely been studied in a scientific manner. Based on the extraction of geographic information of 197 towns in the Hehuang Valley and on variance analysis, this study examines the habitats and subsistence strategies of the Tibetans and other major ethnic groups in the low-latitude region of the Tibetan Plateau. Our statistical results show that the annual average temperature of Tibetan habitats in the Hehuang Valley is relatively high. The relatively warm environment of the valley allows the Tibetans in that area to cultivate wheat and barley and raise cattle and sheep—a subsistence strategy significantly different from that of other Tibetans in the high-altitude regions in the Tibetan Plateau. In addition, the Tibetan communities in the Hehuang Valley include similar agri-pastoral ethnic groups (including Hui and Salar), both of which adopt similar subsistence strategies. The agricultural ethnic groups (Han and Tu) live in a relatively cool and humid environment facilitating agricultural production, while the agri-pastoral ethnic groups (Tibetan, Hui, and Salar) inhabit relatively warm and arid environment in the valley. Due to the lack of agricultural activities, agri-pastoral groups must also engage in animal husbandry to supplement their diet. In the Ando Tibetan region, the subsistence strategies of the ethnic groups are closely related to their physical environment.Those ethnic groups communicate among themselves, integrate, and influence each other, resulting in a diversified culture. This study proves that the habitat variation at a regional scale corresponds significantly to the variation of subsistence strategies. Our findings may further refine knowledge about the human-environmental relationships of Tibetans and lead future research towards using quantitative methods to analyse the intersection of physical environment and ethnic groups' distribution. | Xin JIA Harry F.LEE Mengchun CUI Guoquan CHENG Yang ZHAO Hong DING Ricci P.H.YUE Huayu LU | 2019 | Science China Earth Sciences2019，62，2： | 2 |
| 11 | 马立克氏病新一代疫苗的研究与应用显示文摘马立克氏病(Marek’s disease,MD)是由疱疹病毒马立克氏病病毒(MDV)引起的鸡及其它禽类全身性淋巴瘤病或多克隆T细胞淋巴瘤病。俄罗斯、中国、葡萄牙、摩洛哥、秘鲁等国目前仍有MD暴发;美国、加拿大、英国等欧洲国家鸡群普遍接种MD疫苗,目前MD已得到很好控制。 | Lucy F.Lee | 2010 | 中国家禽2010，32，19： | 2 |
| 12 | Explicit limit equilibrium solution for slope stability显示文摘 | D. Y.Zhu C. F.Lee | 2002 | Geomech2002，，15： | 1 |
| 13 | 查看详情显示文摘 | Park I Lim Y Noh S Lee D.Meister M.Amsden J.J.Laquai F.Lee C.Yoon D.Y | | 0，，： | 1 |
| 14 | Universal primers for fluorescent labelling of PCR fragments—an efficient and cost‐effective approach to genotyping by fluorescence显示文摘 | M. J.BLACKET C.ROBIN R. T.GOOD S. F.LEE A. D.MILLER | 2012 | Molecular Ecology Resources2012，，3： | 1 |
| 15 | Evaluation of the Activity and Stability of Alkali-doped Metal Oxide Catalysts for Application to an Intensified Method of Biodiesel Production显示文摘 | Claire S.MacLeod Adam P.Harvey Adam F.Lee Karen Wilson | | 0，，01： | 1 |
| 16 | Functional genomics approaches in parasitic helminths显示文摘 | J.HAGEN E. F.LEE W. D.FAIRLIE B. H.KALINNA | 2012 | Parasite Immunology (鈥?)2012，，2： | 1 |
| 17 | Activity of plant flavonoids against antibiotic‐resistant bacteria显示文摘 | Hong‐XiXu Song F.Lee | 2001 | Phytother Res2001，，1： | 1 |
| 18 | Effects of carbon taxes on different industries by fuzzy goal programming:A case study of the petrochemical-related industries,Taiwan显示文摘 | Cheng F.Lee Sue J.Lin Charles Lewis Yih F.Chang | | 0，，： | 1 |
| 19 | Coupling and decoupling theory and its application to the MRI phased array显示文摘 | Ray F.Lee Randy O.Giaquinto Christopher J.Hardy | 2002 | Magn. Reson. Med2002，，1： | 1 |
| 20 | Patterns of sperm use by twice-mated female Queensland fruit flies显示文摘Multiple mating by females,polyandry,is common in insects,including in tephritid fruit flies.Female insects that remate commonly store sperm of multiple males.How the sperm of different males contribute to paternity is an important element of sexual selection.Sexual behavior and reproduction of the Queensland fruit fly(Qfly),Bactrocera tryoni,has been extensively investigated both in relation to understanding this economically important species’reproductive biology and in relation to implications for Sterile Insect Technique(SIT),whereby sterile flies are released to constrain reproduction of pest populations.Despite numerous studies of pre-and postcopulatory sexual selection in Qfly,there have been no direct studies of paternity patterns in polyandrous female Qflies.We used two morphologically distinguishable lines to investigate patterns of sperm use in Qfly.The two lines showed comparable mating performance evidenced by similar mating and remating frequency,copula duration,and proportion of second mate paternity(P2)between reciprocal crosses.The mechanism of sperm usage,with P2 close to 0.5 immediately after the second mating followed by gradual decrease of P2 as females aged,is most consistent with stratification or repositioning of sperm.Patterns observed in the present study are compared with the available information from other tephritid fruit flies,and are discussed in relation to this species’reproductive biology,known patterns of sperm storage,and SIT. | Jason Shadmany Siu F.Lee Thu N.M.Nguyen Phillip W.Taylor | 2022 | Insect Science2022，29，4： | 0 |
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