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| 1 | Enhanced Vitamin C Production Mediated by an ABA-Induced PTP-like Nucleotidase Improves Plant Drought Tolerance in Arabidopsis and Maize显示文摘Abscisic acid(ABA)is a key phytohormone that mediates environmental stress responses.Vitamin C,or L-ascorbic acid(AsA),is the most abundant antioxidant protecting against stress damage in plants.How the ABA and AsA signaling pathways interact in stress responses remains elusive.In this study,we characterized the role of a previously unidentified gene,PTPN(PTP-like Nucleotidase)in plant drought tolerance.In Arabidopsis,(AtPTPN was expressed in multiple tissues and upregulated by ABA and drought treatments.Loss-of-function mutants oiAtPTPN were hyposensitive to ABA but hypersensitive to drought stresses,whereas plants with enhanced expression AtPTPN showed opposite phenotypes to.Overexpression of maize PTPN(ZmPTPN)promoted,while knockdown oiZmPTPN inhibited plant drought tolerance,indicating conserved and positive roles of PTPN in plant drought tolerance.We found that both AtPTPN and ZmPTPN release Pi by hydrolyzing GDP/GMP/dGMP/IMP/dIMP,and that AtPTPN positively regulated AsA production via endogenous Pi content control.Consistently,overexpression of VTC2,the rate-limiting synthetic enzyme in AsA biosynthesis,promoted AsA production and plant drought tolerance,and these effects were largely dependent on AtPTPN activity.Furthermore,we demonstrated that the heat shock transcription factor HSFA6a directly binds the AtPTPN promoter and activates AtPTPN expression.Genetic analyses showed that AtPTPN is required for HSFA6a to regulate ABA and drought responses.Taken together,our data indicate that PTPN-mediated crosstalk between the ABA signaling and AsA biosynthesis pathways positively controls plant drought tolerance. | Hui Zhang Yanli Xiang Neng He Xiangguo Liu Hongbo Liu Liping Fang Fei Zhang Xiaopeng Sun Delin Zhang Xingwang Li William Terzaghi Jianbing Yan Mingqiu Dai | 2020 | Molecular Plant2020，13，5： | 7 |
| 2 | Clemens von Sonntag显示文摘 | Xingwang Fang Fertraud Mark | 1996 | Ultrasoni-ca Sonochemistry1996，，3： | 1 |
| 3 | Oxidative decomposition of pentachlorophenol in aqueous solution 显示文摘 | FANG Xingwang HE Yongke LIU Jun | 1998 | Radiation Physics and Chemistry1998，53，： | 1 |
| 4 | Space Materials Science in China:Ⅱ.Ground-based Researches and Academic Activities显示文摘Activities of space materials science research in China have been continuously supported by two main national programs.One is the China Space Station(CSS)program since 1992,and the other is the Strategic Priority Program(SPP)on Space Science since 2011.In CSS plan in 2019,eleven space materials science experimental projects were officially approved for execution during the construction of the space station.In the SPP Phase Ⅱ launched in 2018,seven pre-research projects are deployed as the first batch in 2018,and one concept study project in 2019.These pre-research projects will be cultivated as candidates for future selection as space experiment projects on the recovery of scientific experimental satellites in the future.A new apparatus of electrostatic levitation system for ground-based research of space materials science and rapid solidification research has been developed under the support of the National Natural Science Foundation of China.In order to promote domestic academic activities and to enhance the advancement of space materials science in China,the Space Materials Science and Technology Division belong to the Chinese Materials Research Society was established in 2019.We also organized scientists to write five review papers on space materials science as a special topic published in the journal Scientia Sinica to provide valuable scientific and technical references for Chinese researchers. | PAN Mingxiang WANG Weihua FAN Shuqian ZHANG Qi PAN Xiuhong DENG Weijie HU Liang WEI Bingbo WANG Haipeng YIN Zhigang FANG Jinghong YU Jianding ZHANG Xingwang YUAN Zhangfu JIANG Hongxiang ZHAO Jiuzhou WANG Gong | 2020 | 空间科学学报2020，40，5： | 0 |
| 5 | Development of MEMS directed evolution strategy for multiplied throughput and convergent evolution of cytochrome P450 enzymes显示文摘Directed evolution(DE)inspired by natural evolution(NE)has been achieving tremendous successes in protein/enzyme engineering.However,the conventional'one-protein-for-one-task'DE cannot match the'multi-proteins-for-multi-tasks'NE in terms of screening throughput and efficiency,thus often failing to meet the fast-growing demands for biocatalysts with desired properties.In this study,we design a novel'multi-enzymes-for-multi-substrates'(MEMS)DE model and establish the proof-ofconcept by running a NE-mimicking and higher-throughput screening on the basis of'two-P450 s-against-seven-substrates'(2P×7S)in one pot.With the multiplied throughput and improved hit rate,we witness a series of convergent evolution events of the two archetypal cytochrome P450 enzymes(P450 BM3 and P450 cam)in laboratory.It is anticipated that the new strategy of MEMS DE will find broader application for a larger repertoire of enzymes in the future.Furthermore,structural and substrate docking analysis of the two functionally convergent P450 variants provide important insights into how distinct P450 active-sites can reach a common catalytic goal. | Li Ma Fengwei Li Xingwang Zhang Hui Chen Qian Huang Jing Su Xiaohui Liu Tianjian Sun Bo Fang Kun Liu Dandan Tang Dalei Wu Wei Zhang Lei Du Shengying Li | 2022 | Science China(Life Sciences)2022，65，3： | 0 |
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