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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Timing of volcanism in the Sierra Nevada of California:Evidence for Pliocene delamination of the batholithic root?显示文摘 | Manley C R Glazner A F Farmer G L | 2000 | Geology2000，28，： | 1 |
| 3 | Did lithospheric delamination trigger late Cenozoic potassic volcanism in the southern Sierra Nevada,California?显示文摘 | Farmer G L Glazner A F Manley C R | 2002 | Geological Society of America Bulletin2002，114，： | 1 |
| 4 | CD62 blockade with P-Selectin glycoprotein ligand-immunoglobulin fusion protein reduces ischemia-reperfusion injury after rat intestinal transplantation显示文摘 | FARMER D G SHEN X D AMERSI F | 2005 | Transplantation2005，79，1： | 1 |
| 5 | Assay validation for KIM-I: human urinary renal dysfunction biomarker显示文摘 | CHATURVEDI S FARMER T KAPKE G F | 2009 | Int J Biol Sei2009，5，2： | 1 |
| 6 | Effective damping for SSR analysis of parallel turbine-generators显示文摘 | AGRAWAL B L FARMER F G | 1988 | IEEE Trans on Power Systems1988，3，4： | 1 |
| 7 | Synthesis and biological activity of novel nonsteroidal progesterone receptor antagonists based on cyclocymopol monomethyl ether 显示文摘 | Hamann L G Farmer L F Johnson M G | 1996 | J Med Chem1996，39，9： | 1 |
| 8 | Timing of Volcanism in the Sierra Nevada of California: Evidence for Pliocene delamiation of the batholithic root? 显示文摘 | Manley C R Glazner A F Farmer G L | 2000 | Geologv2000，28，9： | 1 |
| 9 | Effective damping for SSR analysis of parallel turbine-generators显示文摘 | AGRAWAL B L FARMER F G | 1988 | IEEE Trans on Power Systems1988，3，4： | 1 |
| 10 | Plasma amino acids, prolactin, insulin and glucose concentrations in lactating sows following venous infusion of isoleucine, leucine, lysine, threonine or valine显示文摘 | Ridder K A G Farmer C de Lange C F M | 2014 | Canadian Journal of Animal Science2014，94，2： | 1 |
| 11 | Didlithospheric delamination trigger late Cenozoic potassic volcanismin the southern Sierra Nevada,California?显示文摘 | Lang Farmer G Allen F Glazner Curtis R Manley | 2002 | GSA Bulletin2002，11，： | 1 |
| 12 | An analytic approach to practical state space reconstruction 显示文摘 | John F G Farmer J D Casdagli M | 1992 | Physica D1992，57，： | 1 |
| 13 | Current concepts in ischemia and reperfusion injury in the liver显示文摘 | FARMER D G AMERSI F BUSUTTIL R W | 2000 | Transplant Rev2000，14，2： | 1 |
| 14 | One hundred in situ split-liver transplantations:a single-center experience显示文摘 | Yersiz Hansan Renz John F Farmer Douglas G | 2003 | Annals of Surgery2003，238，4： | 1 |
| 15 | Current status of isehemia and reperfusion injury in the liver 显示文摘 | Farmer D G Amersi F Kupiec-Weglinski J W Busuttil R W | 2000 | Transplant Rev2000，14，： | 1 |
| 16 | Oxygen consumption of Tilapia nilotica in relation to swimming speed and salinity显示文摘 | Farmer G J Beamish F W H | 1969 | J Fish Res Bdcan1969，26，： | 1 |
| 17 | An introduction to radio ducting显示文摘 | TURTON J D BENNETTS D A FARMER S F G | 1988 | Meteorological Magazine1988，117，： | 1 |
| 18 | Assay Validation for KIM -1: human urinary renal dysfunction biomarker显示文摘 | Chalurvedi S Farmer T Kapke G F | 2009 | Int J Biol Sci2009，5，2： | 1 |
| 19 | Oxygen consumption of Tilapia nilotiea in relation to swimming speed and salinity 显示文摘 | FARMER G J BEAMISH F W H | 1969 | J Fish Res Board Can1969，26，： | 1 |
| 20 | The preparation of chlorinated rubbers from latex:Hypochlorination and chlorination processes显示文摘 | Bloomfield G F Farmer E H | 1934 | J Soc Chem Ind1934，53，6： | 1 |
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