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	题名	作者	年代	出处	被引量
1	Molecular phenotypes of human parvovirus B19 in patients with myocarditis显示文摘AIM:To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy(DCM).METHODS:Endomyocardial biopsies(EMBs) from 498 B19V-positive patients with myocarditis and DCMwere analyzed using molecular methods and functional experiments.EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers.Control tissues were obtained at autopsy from 34 victims of accidents,crime or suicide.Identification of mononuclear cell infiltrates in EMBs was performed using immunohistological staining.Anti-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay(ELISA).B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction(PCR).For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism(RFLP)-PCR was established.B19V-genotyping was verified by direct DNAsequencing and sequences were aligned using BLAST and BioEdit software.B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture experiments.Transfection experiments were conducted using human endothelial cells 1.Luciferase reporter assays were performed to determine B19Vreplication activity.Statistical analysis and graphical representation were calculated using SPSS and Prism5 software.RESULTS:The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy(iCMP) compared to uninflamed DCM(59.6% vs 35.3%)(P < 0.0001).The detection of B19V-mRNA replication intermediates proved that replication of B19V was present.RFLP-PCR assays showed that B19V-genotype 1(57.4%) and B19V-genotype 2(36.7%) were the most prevalent viral genotypes.B19V-genotype 2 was observed more frequently in EMBs with iCMP(65.0%) compared to DCM(35%)(P = 0.049).Although there was no significant difference in gender-specific B19V-loads,women were more frequently infected with B19V-genotype 2(44.6%) than men(36.0%)(P = 0.0448).Coinfection with B19V and other cardiotropic viruses was found in 19.2% of tissuesamples and was associated with higher B19V viral load compared to B19V-monoinfected tissue(P = 0.0012).The most frequent coinfecting virus was human herpes virus 6(HHV6,16.5%).B19V-coinfection with HHV6 showed higher B19V-loads compared to B19V-monoinfected EMBs(P = 0.0033),suggesting that HHV6 had transactivated B19V.In vitro experiments confirmed a 2.4-fold increased B19V P6-promoter activity by the HHV6 U94-transactivator.CONCLUSION:The finding of significantly increased B19V loads in patients with histologically proven cardiac inflammation suggests a crucial role of B19V-genotypes and reactivation of B19V-infection by HHV6-coinfection in B19V-associated iCMP.Our findings suggest that B19V-infection of the human heart can be a causative event for the development of an endothelial cell-mediated inflammatory disease and that this is related to both viral load and genotype.	C-Thomas Bock Anja Düchting Friederike Utta Eva Brunner Bui Tien Sy Karin Klingel Florian Lang Meinrad Gawaz Stephan B Felix Reinhard Kandolf	2014	World Journal of Cardiology2014，6，4：	3
2	Comparison of accelerated solvent extraction with conventional solvent extraction for organophosphorus pesticides and herbicides显示文摘	Ezzell J L Richter B E Felix W D	1995	LC/GC1995，13，5：	1
3	An Accelerated Solvent Extraction System for the Rapid Preparation of Environmental Organic Compounds in Soil显示文摘	Richter B E Ezzell J L Felix D	1995	Am Lab1995，27，4：	1
4	Trends in polymer electrolytes for secondary lithium batteries显示文摘	Felix B D Lambertus P Jakobert B J V	2000	J Power Sources2000，88，2：	1
5	Influence of large amounts of nitrogen on nonirrigated and irrigated soybean显示文摘	Jeffery D Ray Larry G H Felix B F	2006	Crop Sci2006，46，：	1
6	Early mutational activation of the C-ki-ras oncogene in endometrial carcinoma显示文摘	Duggan B D Felix J C Muderspach L I	1994	Cancer Res1994，54，6：	1
7	Human papillomavirus genotype attribution in invasive cervical cancer:a retrospective cross-sectional worldwide study显示文摘	de Sanjose S Quint WG Alemany L Geraets DT Klaustermeier JE Lloveras B Tous S Felix A Bravo LE Shin HR Vallejos CS de Ruiz PA Lima MA Guimera N Clavero O Alejo M Llombart-Bosch A Cheng-Yang C Tatti SA Kasamatsu E Iljazovic E Odida M Prado R Seoud M Grce M Usubutun A Jain A Suarez GA Lombardi LE Banjo A Menéndez C Domingo EJ Velasco J Nessa A Chichareon SC Qiao YL Lerma E Garland SM Sasagawa T Ferrera A Hammouda D Mariani L Pelayo A Steiner I Oliva E Meijer CJ AlJassar WF Cruz E Wright TC Puras A Llave CL Tzardi M Agorastos T Garcia-Barriola V Clavel C Ordi J Andújar M Castellsagué X Sánchez GI Nowakowsld AM Bornstein J Mu(n)oz N Bosch FX Retrospective International Survey and HPV Time Trends Study Group	2010	Lancet Oncol2010，11，11：	1
8	Effects of testosterone on the electrical properties and nicotinic transmission of the major pelvic and coeliac ganglion neurones显示文摘	Felix B Catalin D Miolan JP	2001	J Neuroendocrinol2001，13，2：	1
9	Preferential Transcriptional Activity of a Glycine-Rich Protein Gene From Arabidopsis thaliana in ProtodermDerived Cells 显示文摘	Saehetto-Martins G Femandes L D Felix D B	1995	International Journal of Plant Sciences1995，156，：	1
10	Feasibility study of brazing aluminium alloys through pre - deposition of a braze alloy by cold spry process 显示文摘	Zhao L D Kirsten B Felix E	2006	Advance Engineering Materials2006，8，8：	1
11	Trends in polymer electrolytes for secondary lithium batteries显示文摘	FELIX B D LAMBERTUS Plomp JAKOERT B J	2000	Power Sources2000，88，：	1
12	Effects of testosterone on the electrical properties and nicotinic transmission of the major pelvic and coeliac ganglion neurones显示文摘	Felix B Catalin D Miolan JP	2001	J Neuroendocrinol2001，13，2：	1
13	An Accelerated Solvent Extraction System for the Rapid Preparation of Environmental Organic Compounds in Soil显示文摘	RICHTER B E EZZELL J L FELIX D	1995	American Laboratory1995，27，4：	1
14	Utilization of Nigerian natural gas resource: potentials and opportunities 显示文摘	Felix B D Anthony O A	1988	Energy Policy1988，16，2：	1
15	Percutaneous renal denervation in patients with treatment-resistant hypertension: final 3-year report of the Symplicity HTN-1 study显示文摘	Henry Krum Markus P Schlaich Michael B?hm Felix Mahfoud Krishna Rocha-Singh Richard Katholi Murray D Esler	2013	The Lancet2013，，：	1
16	Trehalose as cryoprotectant for preservation of yeast strains显示文摘	Coutinho C E B Felix D Panek A D	1988	J Biotechnol1988，7，1：	1
17	Trends in polymer electrolytes for secondary lithium batteries显示文摘	FELIX B D LAMBERTUS P JAKOBERT B J V	2000	J Power Sourcer2000，88，：	1
18	A comparison of accelerated solvent extraction with conventional solvent extraction for organophosphorus pesticides and herbicides 显示文摘	EZZELL J L RICHTER B E FELIX W D	1995	LC/GC1995，13，：	1
19	A comparison of accelerated solvent extraction with conventional solvent extraction for organophosphorus pesticides and herbicides 显示文摘	EZZELL J L RICHTER B E FELIX W D	1995	LC/GC1995，13，：	1
20	Short-term clinical effects of adjunctive antimicrobial photodynamic therapy in periodontal treatment: a randomized clinical trial显示文摘	Andreas B Claudia D Felix K	2008	J Clin Periodontol2008，35，10：	1

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