| 2 | Binding of Euplotes octocarinatus centrin with target peptide melittin显示文摘Interactions between model target peptide melittin (ME) and Euplotes octocarinatus centrin (EoCen) were investigated by fluorescence spectra, circular dichroism (CD) spectra and native polyacrylamide gel electrophoresis (PAGE). In 0.1 mol/L N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (Hepes) and 150 mmol/L NaCl at pH 7.4, EoCen and isolated short C-terminal domain of EoCen (SC-EoCen) form 1:1 peptide:protein complexes. However, no detectable signal changes can be observed while isolated N-terminal domain of EoCen (N-EoCen) or isolated long C-terminal domain of EoCen (LC-EoCen) was added into solution of ME. The interaction between EoCen and ME is specified exclusively for the short C-terminal domain of EoCen. On the basis of fluorescence titration curves, the conditional binding constants of ME with EoCen and SC-EoCen were calculated to be logKME-EoCen = 6.81±0.33 and log- KME-SC-EoCen = 6.51±0.45, respectively. | ZHAO YaQin FENG JiuYing LIANG AiHua YANG BinSheng | 2007 | Chinese Science Bulletin2007，52，23： | 1 |
| 3 | Morphological Difference and DNA Diversity Between Flower-colour Sport and Original Cultivar of Chrysanthemum with Small Flowers显示文摘A total of 58 morphological characteristics were measured on flower-colour sport and original cultivar according to Disticness,Uniformity,Stability(DUS) Test for New Plant Cultivar of Chrysanthemum published by Ministry of Agriculture of China. The results showed that five characteristics such as the surface colour,the back color of ray floret among them were siginificantly different,their MS(morphological similarity) was 91.4%,and MD(morphological difference) was 8.62%. DNA polymorphic analysis by AFLP showed that GS(genetic similarity) was 98.6%,and GD(genetic polymorphic diversity) was 2.81%. Five distinct bands which may include the flower-colour sport genes or be the molecular marker linkaged flower-color characteristics were amplified. | QIN Helan FENG Jiuying | 2011 | Journal of Northeast Agricultural University(English Edition)2011，18，4： | 0 |
| 4 | Behavioral Decision-Making of Key Stakeholders in Public-Private Partnerships:A Hybrid Method and Benefit Distribution Study显示文摘Public-private partnerships(PPPs)have been used by governments around the world to procure and construct infrastructural amenities.It relies on private sector expertise and funding to achieve this lofty objective.However,given the uncertainties of project management,transparency,accountability,and expropriation,this phenomenon has gained tremendous attention in recent years due to the important role it plays in curbing infrastructural deficits globally.Interestingly,the reasonable benefit distribution scheme in a PPP project is related to the behavior decisionmaking of the government and social capital,aswell as the performance of the project.In this paper,the government and social capital which are the key stakeholders of PPP projects were selected as the research objects.Based on the fuzzy expected value model and game theory,a hybrid method was adopted in this research taking into account the different risk preferences of both public entities and private parties under the fuzzy demand environment.To alleviate the problem of insufficient utilization of social capital in a PPP project,this paper seeks to grasp the relationship that exists between the benefit distribution of stakeholders,their behavioral decision-making,and project performance,given that they impact the performance of both public entities and private parties,as well as assist in maximizing the overall utility of the project.Furthermore,four game models were constructed in this study,while the expected value and opportunity-constrained programming model for optimal decision-making were derived using alternate perspectives of both centralized decision-making and decentralized decision-making.Afterward,the optimal behavioral decision-making of public entities and private parties in four scenarios was discussed and thereafter compared,which led to an ensuing discussion on the benefit distribution system under centralized decision-making.Lastly,based on an example case,the influence of different confidence levels,price,and fuzzy uncertainties of PPP projects on the equilibrium strategy results of both parties were discussed,giving credence to the effectiveness of the hybrid method.The results indicate that adjusting different confidence levels yields different equilibriumpoints,and therefore signposts that social capital has a fair perception of opportunities,as well as identifies reciprocal preferences.Nevertheless,we find that an increase in the cost coefficient of the government and social capital does not inhibit the effort of both parties.Our results also indicate that a reasonable benefit distribution of PPP projects can assist them in realizing optimum Pareto improvements over time.The results provide us with very useful strategies and recommendations to improve the overall performance of PPP projects in China. | Guoshuai Sun Wanyi Zhang Jiuying Dong Shuping Wan Jiao Feng | 2023 | Computer Modeling in Engineering & Sciences2023，，9： | 0 |
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