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15篇 您的检索式:作者名="FIEDLE"
    题名 作者 年代 出处 被引量
1Mass spectrometry meets chip technology: a new proteomic tool in cancer research?显示文摘Eggeling F Junker K Fiedle W 2001Electrophoresis2001,22,14:1
2Mass spectrometry meets chip technology:a new proteomic tool in cancer research显示文摘yon Eggeling F Junker K Fiedle W 2001Electrophresis2001,22,14:1
3Mass spectrometry meets chip technology:a new proteomic tool in cancer research? 显示文摘Von Eggeling F Junker K Fiedle W 2001Electrophoresis2001,22,:1
4Mass spectrometry meet chip technology:a new proteomic tool in cancer research显示文摘Eggeling F Junker K Fiedle W 2001Electrophoresis2001,22,14:1
5Mass spectrometry meets chip technology:a new proteomic tool in cancer research显示文摘Eggeling F Junker K Fiedle W 2001Electrophoresis2001,22,14:1
6Telomeres and telomerase:biological and c linical importance显示文摘 Fiedle W Ernst G 1997Pathologe1997,18,6:1
7Mass spectrometry meets chip technology:a new proteomic tool in cancer research显示文摘 Junker K Fiedle W 2001Electrophoresis2001,22,14:1
8Mass spectrometry meet chip technology: a new proteomic tool in cancer research 显示文摘Eggeling F Junker K Fiedle W 2001Electrophoresis2001,22,14:1
9Mass spectrometry meets chip technology:a new proteomic tool in cancer research显示文摘Eggeling F Junker K Fiedle W 2001Electrophoresis2001,22,14:1
10Latitudinal and bathymetric patterns in the distribution and abundance of mesopelagic fish in the Scotia Sea显示文摘Collins M A Stowasser G Fiedling S 2012Deep Sea Research Part II:Topical Studies in Oceanography2012,59,1:1
11Mass spectrometry meets chip technology:a new proteomic tool in cancer research显示文摘Eggeling F Junker K Fiedle W 2001Electrophoresis2001,22,14:1
12Magnetic properties of cube textured silicon-iron magnetic sheet显示文摘Walter J L Hibbard W R Fiedle H C 1958Journal of Applied Physics1958,29,3:1
13Buckwheat:file source of aalioxidant activity in functional foods显示文摘HOLASOVA M FIEDLE ROVA V SMRCINOVA H 2002Food ResearchInternational2002,35,:1
14Mass spectrometry meets chip technology:a new proteomics tool in cancer research? 显示文摘yon Eggeling F Junker K Fiedle W 2001Electrophoresis2001,22,14:1
15土拨鼠肝炎病毒核心蛋白的高效表达和B细胞表位鉴定显示文摘目的建立高效表达土拨鼠肝炎病毒核心蛋白(WHcAg)的方法并对其B细胞表位进行鉴定。方法分别构建不同截短型WHcAg的质粒以了解能大量表达并形成WHV核心颗粒的区段,利用变性WHcAg制备单克隆抗体以寻找能与WHcAg线性B细胞表位结合的单克隆抗体。结果WHcAg前144或149氨基酸残基能够大量表达并能形成颗粒样结构。这2种截短型WHcAg能够在大肠杆菌中表达并组装成直径为34 nm的核心颗粒,最终纯化获得毫克级的核心蛋白。截短型WHcAg保留了全长WHcAg的抗原性,而且变性WHcAg存在另外的B细胞线性表位,利用变性WHcAg进行免疫制备单克隆抗体获得的5株抗体均针对WHcAg的N末端表位,该表位在WHcAg和HBcAg高度保守。结论建立了高效表达WHcAg的方法,制备了针对WHcAg单克隆抗体。并对WHcAg的线性B细胞表位进行了鉴定,发现WHcAg和HBcAg的N末端存在共同的线性B细胞表位。张振华 田拥军 李磊 Melanie Fiedle Ernst Schmid Michael Roggendorf 陆蒙吉 徐飏 杨东亮 2007中华微生物学和免疫学杂志2007,27,6:0
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