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| 1 | Changes of NF-kB,p53,Bcl-2 and caspase in apoptosis induced by JTE-522 in human gastric adenocarcinoma cell line AGS cells:role of reactive oxygen species显示文摘AIM: To identify whether JTE-522 can induce apoptosis inAGS cells and ROS also involved in the process, and toinvestigate the changes in NF-kB, p53, bcl-2 and caspase in the apoptosis process.METHODS: Cell culture, MTT, Electromicroscopy, agarosegel electrophoresis, lucigenin, Western blot andelectrophoretic mobility shift assay (EMSA) analysis wereemployed to investigate the effect of JTE-522 on cellproliferation and apoptosis in AGS cells and relatedmolecular mechanisms.RESULTS: JTE-522 inhibited the growth of AGS cells andinduced the apoptosis. Lucigenin assay showed the generationof ROS in cells under incubation with JTE-522. The inc eareasedROS generation might contribute to the induction of AGS cellsto apoptosis. EMSA and Westem blot revealed that NF-kBactivity was almost completely inhibited by preventing thedegradation of IkBα. Additionally, by using Western blot weconfirmed that the level of bcl-2 was decreased, whereas p53showed a great increase following JTE-522 treatment. Theirchanges were in a dose-dependent manner.CONCLUSION: These findings suggest that reactive oxygenspecies, NF-kB, p53, bcl-2 and caspase-3 may play animportant role in the induction of apoptosis in AGS cellsafter treatment with JTE-522. | Hong-Liang Li Xiao-Hong Li Yan-Qing L Chun-Ling Ye Xian-Da Ren,Department of Pharmacology,Jinan University Pharmacy College,Guangzhou 510632,Guangdong,China Dan-Dan Chen,Department of Cardiology,First Affiliated Hospital,Zhongshan University,Guangzhou 510089,Guangdong,China Hai-Wei Zhang,Department of Pathology,Jinan University Medical College,Guangzhou 510632,Guangdong,China | 2002 | World Journal of Gastroenterology2002，8，3： | 58 |
| 2 | JTE-522-induced apoptosis in human gastric adenocarinoma cell line AGS cells by caspase activation accompanying cytochrome C release,membrane translocation of Bax and loss of mitochondrial membrane potential显示文摘瞄准:在 JTE-522-induced apoptosis 调查 mitochondrial 小径的角色并且调查在细胞色素 C 版本, caspase 活动和 mitochondrial 膜潜力(Deltapsim ) 的损失之间的关系。方法:房间文化,数的房间, ELISA 试金, TUNEL,流动 cytometry,西方的污点和 fluorometric 试金被采用在 AGS 房间和相关分子的机制在房间增长和 apoptosis 上调查 JTE-522 的效果。结果:JTE-522 禁止了 AGS 细胞的生长并且导致了 apoptosis。Caspases 8 并且 9 在从 procaspase 和 caspase 活动由劈开产品的外观判定了劈开特定的 fluorogenic 底层的 apoptosis 期间被激活。阐明是否 caspases 的激活 8 和 9 为 apoptosis 正式就职被要求,我们在 apoptosis 上检验了 caspase 特定的禁止者的效果。结果证明 caspase 禁止者显著地禁止了 JTE-522 导致的 apoptosis。另外,与 Rhodamin 123 的举起的减少伴随的细胞色素 C 的 Bax 和 cytosolic 版本的膜 translocation,在 apoptosis 的一个早阶段被检测。而且, Bax translocation,细胞色素 C 版本,和 caspase 9 激活被 Z-VAD.fmk 和 Z-IETD-CHO 堵住。结论:现在的数据显示在 caspases 的激活之间的一个关键协会 8, 9,细胞色素 C 版本, Bax 的膜 translocation, Deltapsim 的损失和在 AGS 房间的 JTE-522-induced apoptosis。 | Hong-Liang Li Xiao-Hong Li Jun-Hua Lü Xian-Da Ren,Department of Pharmacology,Jinan University Pharmacy College,Guangzhou 510632,Guangdong Province,China Dan-Dan Chen,Department of Cardiology,First Affiliated Hospital,Zhongshan University,Guangzhou 510089,Guangdong Province,China Hai-Wei Zhang,Department of Pathology,Jinan University Medical College,Guangzhou 510632,Guangdong Province,China Cun-Chuan Wang,Department of laparoscopic surgery,First Affiliated Hospital,Jinan University Medical College,Guangzhou 510632,Guangdong Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 16 |
| 3 | Tidally induced upwelling off Yangtze River estuary and in Zhejiang coastal waters in summer显示文摘MASNUM wave-tide-circulation coupled numerical model (MASNUM coupled model, hereinafter) is de-veloped based on the Princeton Ocean Model (POM). Both POM and MASNUM coupled model are ap-plied in the numerical simulation of the upwelling off Yangtze River estuary and in Zhejiang coastal waters in summer. The upwelling mechanisms are analyzed from the viewpoint of tide, and a new mechanism is proposed. The study suggests that the tidally inducing mechanism of the upwelling in-cludes two dynamic aspects: the barotropic and the baroclinic process. On the one hand, the residual currents induced by barotropic tides converge near the seabed, and upwelling is generated to maintain mass conservation. The climbing of the residual currents along the sea bottom slope also contributes to the upwelling. On the other hand, tidal mixing plays a very important role in inducing the upwelling in the baroclinic sea circumstances. Strong tidal mixing leads to conspicuous front in the coastal waters. The considerable horizontal density gradient across the front elicits a secondary circulation clinging to the tidal front, and the upwelling branch appears near the frontal zone. Numerical experiments are de-signed to determine the importance of tide in inducing the upwelling. The results indicate that tide is a key and dominant inducement of the upwelling. Experiments also show that coupling calculation of the four main tidal constituents (M2, S2, K1, and O1), rather than dealing with the single M2 constituent, im-proves the modeling precision of the barotropic tide-induced upwelling. | L XinGang1,2,3,4, QIAO FangLi1,2, XIA ChangShui1,2 & YUAN YeLi1,2 1 The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China 2 Key Laboratory of Marine Science and Numerical Modeling (MASNUM), State Oceanic Administration, Qingdao 266061, China 3 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 4 Graduate University of the Chinese Academy of Sciences, Beijing 100039, China | 2007 | Science China Earth Sciences2007，50，3： | 9 |
| 4 | Effects of bile acids on proliferation and ultrastructural alteration of pancreatic cancer cell lines显示文摘AIM: Pancreatic cancer in the head is frequently accompanied by jaundice and high bile acid level in serum. This study focused on the direct effects of bile acids on proliferation and ultrastructural alteration of pancreatic cancer.METHODS: Pancreatic cancer cell lines PANC-1, MIA PaCa2 and PGHAM-1 were explored in this study. The cell lines were cultured in media supplemented with certain bile acids,CA, DCA, LCA, TCDC, TDCA and GCA. Their influence on cell growth was measured with MTT assay after 72 h of incubation. Cell cycles of PANC-1 cells in 40 μM of bile acids media were analyzed by flow cytometry. Ultrastructural alteration of PANC-1 cells induced by DCA was observed using scanning and transmission electron microscope (SEM and TEM).RESULTS: At various concentrations of bile acids and incubation time, no enhanced effects of bile acids on cell proliferation were observed. Significant inhibitory effects were obtained in almost all media with bile acids. DCA and CA increased the percentage of G0+G1 phase cells, while GCA and TDCA elevated the S phase cell number. After 48 h of incubation in DCA medium, PANC-1 cells showed some structural damages such as loss of their microvilli and vacuolization of organelles in cytoplasm.CONCLUSION: Bile acids can reduce proliferation of pancreatic cancer cells due to their direct cytotoxicity. This result implies that elevation of bile acids in jaundiced serum may inhibit pancreatic cancer progression. | Zheng Wu Yi Lü Bo Wang Chang Liu Zuo-Ren Wang Department of Hepatobiliary Surgery,The First Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，12： | 8 |
| 5 | Cloning by somatic cell nuclear transfer显示文摘 | Fulka J Jr First N L Loi P | 1998 | Bio Essays1998，，20： | 1 |
| 6 | Response of porcine oocytes to electrical and chemical activation during maturation in vitro显示文摘 | HAGEN D R PRATHER R S FIRST N L | 1991 | Mol Reprod Develop1991，28，： | 1 |
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| 9 | Timing of Meiotic Progression in Bovine Oocytes and its Effect on Early Embryo Develop- ment 显示文摘 | DOMINKO T FIRST N L | 1997 | Mol ReprodDev1997，47，11： | 1 |
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| 12 | The effects of fluoxetine treatment in a chronic mild stress rat model on depression-related behavior,brain neurotrophins and ERK expression显示文摘 | FIRST M GIL AD L TALER M | 2011 | J Mol Neurosci2011，45，2： | 1 |
| 13 | The application of ultraviolet germicidal irradiation to control teansmission of airbome disease: bioterrorism countermeasure 显示文摘 | Brickner P W Vincent R L First M | 2003 | Public Health Rep2003，118，2： | 1 |
| 14 | In vitro inhibition of oocyte nuclear maturation in the bovine显示文摘 | Sirard M A First N L | 1988 | Biol-Reprod1988，39，2： | 1 |
| 15 | Co-cultures of early cattle embryos to the blastocyst stage with oviductal tissue or in conditional medium 显示文摘 | Eyesone W H First N L | 1989 | J Reprod Fertil1989，85，： | 1 |
| 16 | Bovine ocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species显示文摘 | Dominko T Massam M Haley B Beyhan Z Melili E Mckusick B First N L | | 0，，： | 1 |
| 17 | Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocytes maturation 显示文摘 | Fulka J J First N L Moor R M | 1998 | Human Reproduction1998，4，： | 1 |
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| 19 | Developmental and molecular correlates of bovine preimplantation embryos显示文摘 | Sagirkaya H Misirlioglu M Kaya A First N L Parrish J J Memili E | | 0，，5： | 1 |
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