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| 1 | Role of autophagy in differential sensitivity of hepatocarcinoma cells to sorafenib显示文摘AIM: To investigate the role of sorafenib(SFN) in autophagy of hepatocellular carcinoma(HCC). We evaluated how SFN affects autophagy signaling pathway in human HCC cell lines. METHODS: Two different human HCC cell lines, Hep3 B and Huh7, were subjected to different concentrations of SFN. Cell viability and onset of apoptosis were determined with colorimetric assay and immunoblotting analysis, respectively. The changes in autophagy-related proteins, including LC3, ULK1, AMPK, and LKB, were determined with immunoblotting analysis in the presence or absence of SFN. To assess autophagic dynamics, autophagic flux was measured with chloroquine, a lysosomal inhibitor. The autophagic responsiveness between different HCC cell lines was compared under the autophagy enhancing conditions.RESULTS: Hep3 B cells were significantly more resistant to SFN than Huh7 cells. Immunoblotting analysis revealed a marked increase in SFN-mediated autophagy flux in Huh7 cells, which was, however, absent in Hep3 B cells. While both starvation and rapamycin enhanced autophagy in Huh7 cells, only rapamycin increased autophagy in Hep3 B cells. Immunoblotting analysis of autophagy initiation proteins showed that SFN substantially increased phosphorylation of AMPK and consequently autophagy in Huh7, but not in Hep3 B cells.CONCLUSION: The autophagic responsiveness to SFN is distinct between Hep3 B and Huh7 cells. Resistance of Hep3 B cells to SFN may be associated with altered autophagy signaling pathways. | Trevan D Fischer Jin-Hee Wang Adrian Vlada Jae-Sung Kim Kevin E Behrns | 2014 | World Journal of Hepatology2014，6，10： | 5 |
| 2 | Coexpression of receptor-tyrosine-kinases in gastric adenocarcinoma-a rationale for a molecular targeting strategy?显示文摘AIM: To define the (co-)expression pattern of target receptor-tyrosine-kinases (RTK) in human gastric adenocarcinoma. METHODS: The (co-)expression pattern of VEGFR1-3,PDGFRα/b and EGFR1 was analyzed by RT-PCR in 51 human gastric adenocarcinomas. In addition,IHC staining was applied for confirmation of expression and analysis of RTK localisation. RESULTS: The majority of samples revealed a VEGFR1 (98%),VEGFR2 (80%),VEGFR3 (67%),PDGFRα (82%) and PDGFRβ(82%) expression,whereas only 62% exhibited an EGFR1 expression. 78% of cancers expressed at least four out of six RTKs. While VEGFR1-3 and PDGFRα revealed a predominantly cytoplasmatic staining in tumor cells,accompanied by an additional nuclear staining for VEGFR3 ,EGFR1 was almost exclusively detected on the membrane of tumor cells. PDGFRβ was restricted to stromal pericytes,which also depicted a PDGFRα expression.receptor-tyrosine-kinases coexpression in gastric adenocarcinoma and might therefore encourage an application of multiple-target RTK-inhibitors within a combination therapy. | Daniel Drescher Markus Moehler Ines Gockel Kirsten Frerichs Annett Müller Friedrich Dünschede Thomas Borschitz Stefan Biesterfeld Martin Holtmann Thomas Wehler Andreas Teufel Kerstin Herzer Thomas Fischer Martin R Berger Theodor Junginger Peter R Galle Carl C Schimanski | 2007 | World Journal of Gastroenterology2007，13，26： | 4 |
| 3 | MODIFIED MELT TEXTURING PROCESS FOR YBCO BASED ON THE POLYTHERMIC SECTION YO1.5-BA0.4CU0.6O IN THE Y-BA-CU-O PHASE DIAGRAM AT 0.21 BAR OXYGEN PRESSURE显示文摘 | Krabbes G Schatzle P Bieger W Wiesner U Stover G Wu M Strasser T Litzkendorf D Fischer K Gornert P | | 0，，： | 2 |
| 4 | Stem cell division is regulated by the microRNA pathway显示文摘 | HATFIELD S D SHCHERBATA H R FISCHER K A | 2005 | Nature2005，435，7044： | 1 |
| 5 | Cell type specificity of the human endoglin promoter显示文摘 | Guaulich W Nettelbeck D M Fischer D | 1999 | Gene1999，227，1： | 1 |
| 6 | Thermal stresses for frictional contact in wheel-rail system 显示文摘 | Fischer F D Wemer E Yan W Y | 1997 | Wear1997，211，2： | 1 |
| 7 | Development of high performance multifilamentary Nb-Ti-Ta superconductor for LHC insertion quadrupoles 显示文摘 | Lee P J Fischer C M Larbalestier D C | 1999 | IEEE Trans Applied Superconductivity1999，9，2： | 1 |
| 8 | FDG avidity and PET/CT patterns in primary gastric lymphoma显示文摘 | Radan L Fischer D Bar-Shalom R | 2008 | Eur J Nucl Med MolImaging2008，35，8： | 1 |
| 9 | Raffinose improves 24-hour lung preservation in low potassium dextran glucose solution:a histologic and ultrastructural analysis显示文摘 | Fischer S Hopkinson D Liu M Y | 2001 | Ann Thorac Surg2001，71，： | 1 |
| 10 | Parturition at term:parallel increases in interleukin-8 and proteinase concentrations and neutrophil count in the lower uterine segment显示文摘 | Winkler M Fischer D C Ruck P | 1999 | Hum Reprod1999，14，： | 1 |
| 11 | Leptin activates cardiac fatty acid oxidation independent of changes in the AMP-activated protein kinase: acetyl-CoA carboxylase-malonyl- CoA axis 显示文摘 | ATKINSON L L FISCHER M A LOPASCHUK G D | 2002 | J Biol Chem2002，277，29： | 1 |
| 12 | Ubiquitin-conjugating enzyme E2-25K increases aggregate formation and cell death inpolyglutamine diseases显示文摘 | de Pril R Fischer D F Roos R A C | 2007 | Mol Cell Neurosci2007，34，： | 1 |
| 13 | Shading and the growth and photosynthetic responses of Ammann/a coccinnea 显示文摘 | Gibson K D Fischer A J Foin T C | 2001 | Weed Re- seareh2001，41，： | 1 |
| 14 | Acupuncture for idiopathic headaehe显示文摘 | Melchart D Linde K Fischer P | 2001 | Cochrane Database Syst Rev2001，，00： | 1 |
| 15 | Sounds and size:identification of acoustic variables that reflect body size in hamadryas baboons,Papio hamadryas显示文摘 | Pfefferle D Fischer J | 2006 | Animal Behaviour2006，72，1： | 1 |
| 16 | Knowledge interactions between universities and industry in Austria:sectoral patterns and determinants显示文摘 | Schartinger D Rammer C Fischer M M | | 0，，03： | 1 |
| 17 | Diagnostic accuracy of whole-body MRI/DWI image fusion for detection of malignant tumours:a comparison with PET/CT显示文摘 | Fischer MA Nanz D Hany T | | 0，，02： | 1 |
| 18 | Rapid metagenomic diagnostics for suspected outbreak of severe pneumonia 显示文摘 | Fischer N Rohde H Indenbirken D | 2014 | Emerg Infect Dis2014，20，6： | 1 |
| 19 | Cognitive function in pa- tients with decompensated heart failure: the Cognitive Impairment in Heart Failure(Cog Impair-HF) study显示文摘 | Kindermann I Fischer D Karbach J | 2012 | European Journal of Heart Failure2012，14，： | 1 |
| 20 | A broad role for melanopsin in nonvisual photoreception显示文摘 | GOOLE J J LU J FISCHER D | 2003 | J Neurosei2003，23，18： | 1 |
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