|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | Significance of COX-1, COX-2 and iNOS expression in superficial gastritis, gastric mucosa atypical hyperplasia and gastric carcinoma显示文摘 | Luo YQ Wu KC Sun AH Pan BR Zhang XY Fan DM | 2000 | Chin J Digestion(中华消化杂志)2000，20，4： | 1 |
| 3 | Management of spontaneously ruptured hepatocellular carcinoma and he moperitoneum manifested as acute abdomen in the emer gency room显示文摘 | Hsueh KC Fan HL Chen TW | 2012 | World J Surg2012，36，11： | 1 |
| 4 | Tracheal rupture complicating emergent endotracheal intabation显示文摘 | Fan CM Ko PC Taai KC | 2004 | AmJ Emerg Med2004，22，4： | 1 |
| 5 | Seeing older perioperative patients in 32-D: when your patient is demented, delirium, or depressed 显示文摘 | Buck Walter KC Fan RN Buckwalter JA | 2002 | Oper Tech Orthop2002，12，2： | 1 |
| 6 | Interleukin-1 receptor antagonist ameliorates neonatal lipopolysaccharide-in- duced long-lasting hyperalgesia in the adult rats显示文摘 | Wang KC Wang SJ Fan LW | 2011 | Toxi- cology2011，279，13： | 1 |
| 7 | Diagnosis and treatment for primary cases 显示文摘 | Zhao HX Fan KC Yang YS small intestine Iymphoma of 10 2007 27(9): 695-696 | 2007 | Chin J Prac Intern Med2007，，： | 1 |
| 8 | Interleukin- 1 receptor antagonist ameliorates neonatal lipopolysaccharide-induced long- lasting hyperalgesia in the adult rats 显示文摘 | Wang KC Wang S J Fan LW | 2011 | Toxicology2011，279，13： | 1 |
| 9 | Xanthones from Tripterospermum chinense(Migo) 显示文摘 | Zhu KC Ma CH Fan MS | 2007 | Asian J Chem2007，19，3： | 1 |
| 10 | Xanthones from Tripterospermumchinense (Migo) 显示文摘 | Zhu KC Ma CH Fan MS | 2007 | Asian Journal of Chemistry2007，19，3： | 1 |
| 11 | Influence of mRNA decay rates on the computational prediction of transcription rate profiles from gene expression profiles 显示文摘 | Chin CF Shih AC Fan KC | 2007 | J Biosci2007，32，7： | 1 |
| 12 | Interleukin-1receptor antagonist ameliorates neonatal lipopolysaccharide-induced long-lasting hyperalgesia in the adult rats显示文摘 | Wang KC Wang SJ Fan LW | 2011 | Toxicology2011，279，13： | 1 |
| 13 | The clinical report of 15 nonspceific granulomatous prostatitis cases显示文摘 | Fan HT Wang KC Zhang MC | 2008 | Zhonghua Nan Ke Xue2008，14，2： | 1 |
| 14 | Regional quantification of cer- ebral venous oxygenation from MRI susceptibility during hyper- capnia显示文摘 | Fan AP Evans KC Stout JN | 2015 | Neuroimage2015，104，1： | 1 |
| 15 | Metabolic and immunologic features of Chinese patients with atypical diabetes rnellitus显示文摘 | Fan KC Mackay IR Zimmet PZ | 2000 | Diabetes Care2000，23，3： | 1 |
| 16 | Seeing older perioperative patients in3-D:when your patient is demented,delirium,or depressed显示文摘 | Buck walter KC Fan RN Buckwalter JA | 2002 | Operative Techniques in Orthopaedics2002，12，2： | 1 |
| 17 | Xanthones from Tripteros- perrmum chinense (Migo) 显示文摘 | Zhu KC Ma CH Fan MS | 2007 | Asian J Chem2007，19，： | 1 |
| 18 | Effects of somatostatin, octreotide and pitressin plus nitroglycerine on systemic and portal haemodynamics in the control of acute variceal bleeding 显示文摘 | Wong BC Zhou XM Guo XG Zhao SJ Wang JH Wu KC Ding J Lam SK Fan DM | 2002 | Int J Clin Pract2002，56，6： | 1 |
| 19 | The resilience and health sta- tus of primary caregivers of schizophrenia patients 显示文摘 | Fan YC Chen MB Lin KC | 2014 | Hu Li Za Zhi2014，61，6： | 1 |
| 20 | TNF-a mediates eosinophil cationic proteininduced apoptosis in BEAS-2B cells显示文摘 | Chang KC Lo CW Fan TC | 2010 | BMC Cell Biol2010，11，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员