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| 1 | De novo assembly and comparative analysis of root transcriptomes from different varieties of Panax ginseng C. A. Meyer grown in different environments显示文摘Panax ginseng C. A. Meyer is an important traditional herb in eastern Asia. It contains ginsenosides, which are primary bioactive compounds with medicinal properties. Although ginseng has been cultivated since at least the Ming dynasty to increase production, cultivated ginseng has lower quantities of ginsenosides and lower disease resistance than ginseng grown under natural conditions. We extracted root RNA from six varieties of fifth-year P. ginseng cultivars representing four different growth conditions, and performed Illumina paired-end sequencing. In total, 163,165,706 raw reads were obtained and used to generate a de novo transcriptome that consisted of 151,763 contigs(76,336 unigenes), of which 100,648 contigs(66.3%) were successfully annotated. Differential expression analysis revealed that most differentially expressed genes(DEGs) were upregulated(246 out of 258, 95.3%) in ginseng grown under natural conditions compared with that grown under artificial conditions. These DEGs were enriched in gene ontology(GO) terms including response to stimuli and localization. In particular, some key ginsenoside biosynthesis-related genes, including HMG-Co A synthase(HMGS), mevalonate kinase(MVK), and squalene epoxidase(SE), were upregulated in wild-grown ginseng. Moreover, a high proportion of disease resistance-related genes were upregulated in wild-grown ginseng. This study is the first transcriptome analysis to compare wild-grown and cultivated ginseng, and identifies genes that may produce higher ginsenoside content and better disease resistance in the wild; these genes may have the potential to improve cultivated ginseng grown in artificial environments. | ZHEN Gang ZHANG Lei DU YaNan YU RenBo LIU XinMin CAO FangRui CHANG Qi DENG Xing Wang XIA Mian HE Hang | 2015 | Science China(Life Sciences)2015，58，11： | 6 |
| 2 | A variable stepsize INC MPPT method for PV systems 显示文摘 | Liu Fangrui Duan Shanxu Liu Fei | 2008 | IEEETransactions on Industrial Electronics2008，55，7： | 1 |
| 3 | A variable step size INC MPPT method for PV systems 显示文摘 | Fangrui Liu Shanxu Duan Yong Kang | 2008 | IEEE Transactions on Industrial Electronics2008，55，7： | 1 |
| 4 | A variable step size INC MPPT method for PV systems显示文摘 | LIU Fangrui DUAN Shanxu LIU Fei | 2008 | IEEE Transactions on In- dustrial Electronics2008，55，7： | 1 |
| 5 | A unity power-factor converter using the synchronous-reference-frame-based hysteresis current control 显示文摘 | Maswood A I Liu Fangrui | 2007 | IEEE Transactions on Industry Applications2007，43，2： | 1 |
| 6 | A Variable Step Size 1NC MPPT Method for PV Systems 显示文摘 | Fangrui Liu Shanxu Duan Fei Liu Bangyin Liu Yong Kang | 2008 | IEEE Transactions on Industrial Electronics (S0278-0046)2008，55，7： | 1 |
| 7 | A novel variable hysteresis band current control of three-phase three-level unity PF rectifier with constant switching frequency显示文摘 | Fangrui Liu Maswood A I | 2006 | IEEE Trans on Power Electronics2006，21，6： | 1 |
| 8 | A variable step size INC MPPT method for PV systems显示文摘 | Liu Fangrui Duan Shanxu Liu Bangyin | 2008 | IEEE Transactions on Industrial Electronics2008，55，7： | 1 |
| 9 | A variable step size INC MPPT method for PV systems显示文摘 | Liu Fangrui Duan Shanxu Liu Fei | | 0，，07： | 1 |
| 10 | A Variable Step Size INC MPPT Method for PV Systems 显示文摘 | Fangrui Liu Shanxu Duan Fei Liu Bangyin Liu andYongKang | 2008 | IEEE TRANSACTIONS ON INDUSTRIAL ELECTRONICS2008，55，7： | 1 |
| 11 | A Variable Step Size INC MPPT Method for PV Systems 显示文摘 | Liu Fangrui Duan Shanxu Liu Fei | 2008 | IEEE Transactions on industrial Electronics2008，55，7： | 1 |
| 12 | DNA Methylation Mediated by a MicroRNA Pathway显示文摘 | Liang Wu Huanyu Zhou Qingqing Zhang Jianguang Zhang Fangrui Ni Chang Liu Yijun Qi | 2010 | Molecular Cell2010，，3： | 1 |
| 13 | A unity power-factor converter using the synchronous-reference-frame-based hysteresis current control 显示文摘 | Maswood A Liu Fangrui | 2007 | IEEE Trans on Industry Applications2007，43，2： | 1 |
| 14 | A Variable Step Size INC MPPT Method for PV Systems显示文摘 | Fangrui Liu Shanxu Duan Fei Liu | 2008 | IEEE Transactions on Industrial Electronics2008，55，7： | 1 |
| 15 | A variable step size INC MPPT method for PV systems 显示文摘 | Liu Fangrui Duan Shanxu Liu Fei | 2008 | IEEE Trans on Industrial Electronics2008，55，7： | 1 |
| 16 | Coordinated control of cascaded current-source converter based offshore wind farm显示文摘 | Popat M Wu Bin Liu Fangrui | 2012 | IEEE Transactions on Sustainable Energy2012，3，3： | 1 |
| 17 | A variable step size INC MPPT method for PV systems显示文摘 | LIU Fangrui DUAN Shanxu LIU Fei | 2008 | IEEE Transactions on Industrial Electronics2008，55，7： | 1 |
| 18 | A variable step size INC MPPT method of PV systems 显示文摘 | Liu Fangrui Duan Shanxu Liu Fei | 2008 | IEEE transactions on industrial electronics2008，55，7： | 1 |
| 19 | A Variable Step Size INC MPPT Method for PV Systems显示文摘 | LIU Fangrui DUAN Shanxu LIU Fei | | 0，，07： | 1 |
| 20 | Monitoring microenvironment of Hep G2 cell apoptosis using two-photon fluorescence lifetime imaging microscopy显示文摘Apoptosis is very important for the maintenance of cellular homeostasis and is closely related to the occurrence and treatment of many diseases.Mitochondria in cells play a crucial role in programmed cell death and redox processes.Nicotinamide adenine dinucleotide(NAD(P)H)is the primary producer of energy in mitochondria,changing NAD(P)H can directly reflect the physiological state of mitochondria.Therefore,NAD(P)H can be used to evaluate metabolic response.In this paper,we propose a noninvasive detection method that uses two-photon fluorescence lifetime imaging microscopy(TP-FLIM)to characterize apoptosis by observing the binding kinetics of cellular endogenous NAD(P)H.The result shows that the average fluorescence lifetime of NAD(P)H and the fluorescence lifetime of protein-bound NAD(P)H will be affected by the changing pH,serum content,and oxygen concentration in the cell culture environment,and by the treatment with reagents such as H2O2 and paclitaxel.Taxol(PTX).This noninvasive detection method realized the dynamic detection of cellular endogenous substances and the assessment of apoptosis. | Kexin Wang Shiyao Tang Shiqi Wang Fangrui Lin Gengjin Zou Junle Qu Liwei Liu | 2022 | Journal of Innovative Optical Health Sciences2022，15，3： | 1 |
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