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| 1 | Acute inflammation stimulates a regenerative response in the neonatal mouse heart显示文摘 | Chunyong Han Yu Nie Hong Lian Rui Liu Feng He Huihui Huang Shengshou Hu | 2015 | Cell Research2015，25，10： | 26 |
| 2 | Characterization and normalization factors of abiotic resource depletion for life cycle impact assessment in China显示文摘The availability of resources for economic activities differs between regions, and the importance of the resources is consequently observed to be different within regions compared to a global scale. With the current situation in Chinese mining industry and its statistic characteristics, the characterization procedures of abiotic resource in life cycle impact assessment (LCIA) have demonstrated certain limita-tions in the Chinese materials industry. The aim of this paper is to propose new characterization and normalization factors for abiotic resource depletion categories such as metals and non-renewable en- ergy resources in a Chinese context. The actual production of abiotic resources calculated by a modi- fied model is compared to the reserve base in line with the new national standard to determine char- acterization factors in equivalence units, with antimony as the reference mineral. The normalization factors are based on the total base reserves of the most important minerals in China. A case study on primary magnesium production using the Pidgeon process is used to compare LCIA results for abiotic resource categories that are between current LCIA factors and the new Chinese factors. These factors not only reflect the importance of abiotic resource with respect to region-specific resource depletion, but also can compare with the global factors. | GAO Feng NIE ZuoRen WANG ZhiHong GONG XianZheng ZUO TieYong | 2009 | Science China(Technological Sciences)2009，52，1： | 17 |
| 3 | RNA interference:Antiviral weapon and beyond显示文摘RNA interference (RNAi) is a remarkable type of gene regulation based on sequence-specific targeting and degradation of RNA. The term encompasses related pathways found in a broad range of eukaryotic organisms, including fungi, plants, and animals. RNA interference is part of a sophisticated network of interconnected pathways for cellular defense, RNA surveillance, and development and it may become a powerful tool to manipulate gene expression experimentally. RNAi technology is currently being evaluated not only as an extremely powerful instrument for functional genomic analyses, but also as a potentially useful method to develop specific dsRNA based gene-silencing therapeutics.Several laboratories have been interested in using RNAi to control viral infection and many reports in Nature and in Cell show that short interfering (si) RNAs can inhibit infection by HIV-1, polio and hepatitis C viruses in a sequence-specific manner. RNA-based strategies for gene inhibition in mammalian cells have recently been described, which offer the promise of antiviral therapy. | Quan-Chu Wang Qing-He Nie Zhi-Hua Feng, The Center of Diagnosis and Treatment for Infectious Diseases of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China | 2003 | World Journal of Gastroenterology2003，9，8： | 14 |
| 4 | LYRM03, an ubenimex derivative, attenuates LPS- induced acute lung injury in mice by suppressing the TLR4 signaling pathway显示文摘像使用费的受体(TLR4 ) 4 调停了表明戏在导致败血的尖锐的肺损害(ALI ) 的一个关键角色。LYRM03 (3-amino-2-hydroxy-4-phenyl-valyl-isoleucine ) 是 ubenimex 的新奇衍生物,广泛地使用的 antineoplastic 药。我们以前发现 LYRM03 在盲肠的结扎刺老鼠模型有反煽动性的效果。在这研究,我们决定了 LYRM03 是否在老鼠稀释了导致 LPS 的 ALI。导致 LPS 的 ALI 老鼠模型被与 LPS (5 mg/kg ) 的 intratracheal 注射质问老鼠建立,它随后与 LYRM03 被对待(10 mg/kg, ip ) 。LYRM03 管理显著地减轻了导致 LPS 的肺浮肿,煽动性的房间(neutrophils 和巨噬细胞) 渗入和 myeloperoxidase (军邮局) 活动,减少的支持 inflammatory 和趋化性的 cytokine (TNF- , IL-6, IL-1, MIP-2 ) 产生和在肺纸巾的 COX-2 表示。在有教养的老鼠在 vitro 的牙槽的巨噬细胞,有 LYRM03 (100 mol/L ) 的预告的处理由减少 Myd88 表示,增加 IB 稳定性并且禁止 p38 phosphorylation 压制了导致 LPS 的巨噬细胞激活。这些结果建议 LYRM03 有效地由在牙槽的巨噬细胞禁止支持 inflammatory 调停人和 Myd88 依赖的 TLR4 发信号小径的表示稀释导致 LPS 的 ALI。LYRM03 可以为调停败血的肺损害用作一个潜在的处理。 | Hui-qiong HE Ya-xian WU Yun-juan NIE Jun WANG Mei GE Feng QIAN | 2017 | Acta Pharmacologica Sinica2017，38，3： | 14 |
| 5 | Neuroprotective Autophagic Flux Induced by Hyperbaric Oxygen Preconditioning is Mediated by Cystatin C显示文摘We have previously reported that Cystatin C(CysC) is a pivotal mediator in the neuroprotection induced by hyperbaric oxygen(HBO) preconditioning; however,the underlying mechanism and how CysC changes after stroke are not clear. In the present study, we demonstrated that CysC expression was elevated as early as 3 h after reperfusion, and this was further enhanced by HBO preconditioning. Concurrently, LC3-II and Beclin-1, two positive-markers for autophagy induction, exhibited increases similar to CysC, while knockdown of CysC blocked these elevations. As a marker of autophagy inhibition, p62 was downregulated by HBO preconditioning and this was blocked by CysC knockdown. Besides, the beneficial effects of preserving lysosomal membrane integrity and enhancing autolysosome formation induced by HBO preconditioning were abolished in CysC-/-rats.Furthermore, we demonstrated that exogenous CysC reduced the neurological deficits and infarct volume after brain ischemic injury, while 3-methyladenine partially reversed this neuroprotection. In the present study, we showed that CysC is biochemically and morphologically essential for promoting autophagic flux, and highlighted the translational potential of HBO preconditioning and CysC for stroke treatment. | Zongping Fang Yun Feng Yuheng Li Jiao Deng Huang Nie Qianzhi Yang Shiquan Wang Hailong Dong Lize Xiong | 2019 | Neuroscience Bulletin2019，35，2： | 14 |
| 6 | Eleven COVID-19 Outbreaks with Local Transmissions Caused by the Imported SARS-CoV-2 Delta VOC-China,July-August,2021显示文摘Starting from December 2019,Wuhan,China,encountered the first outbreak of coronavirus disease 2019(COVID-19)(1-2).The epidemic was successfully suppressed by strict containment so that the number of infected people was reduced to 0 on April 8,2020(3–4).After that,China experienced roughly 3 dozen outbreaks with local transmission caused by imported severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). | Lei Zhou Kai Nie Hongting Zhao Xiang Zhao Bixiong Ye Ji Wang Cao Chen Hong Wang Jiangli Di Jinsong Li Chao Li Zhixiao Chen Ruiqi Ren Peihua Niu Hui Chen Tao Chen Yali Wang Lili Li Bingxue Han Ruhan A Si Chen Dan Li Dan Li Chunxia Jin Xin Zhang Jiangmei Liu Chunyu Xu Yecheng Yao Yenan Feng Zhili Li Bike Zhang Yang Song Peter Hao Yanping Zhang Hao Li Qun Li George F.Gao Guoqing Shi Wenbo Xu | 2021 | China CDC weekly2021，3，41： | 13 |
| 7 | Chemical vapor deposition growth of large-scale hexagonal boron nitride with controllable orientation显示文摘有一致厚度的大域的六角形的硼氮化物(h-BN ) 的化学蒸汽免职(CVD ) 合成是很挑战性的,主要由于这材料的极其高的成核密度。此处,我们报导晶片规模的成功的生长,有大单人赛水晶的领域尺寸的高质量的 h-BN 单层电影,吗直到~ 72 ??????????????????????????慮潮档湡敮獬???????慮潮畴敢??洠獥灯牯???? ?? | Xiuju Song Junfeng Gao Yufeng Nie Teng Gao Jingyu Sun Donglin Ma Qiucheng Li Yubin Chen Chuanhong Jin Alicja Bachmatiuk Mark H. Rummeli Feng Ding Yanfeng Zhang Zhongfan Liu | 2015 | Nano Research2015，8，10： | 12 |
| 8 | DDIT4 promotes gastric cancer proliferation and tumorigenesis through the p53 and MAPK pathways显示文摘Background:Gastric cancer(GC)is one of the most common malignancies worldwide,particularly in China.DNA damage-inducible transcript 4(DDIT4)is a mammalian target of rapamycin inhibitor and is induced by various cellular stresses;however,its critical role in GC remains poorly understood.The present study aimed to investigate the poten-tial relationship and the underlying mechanism between DDIT4 and GC development.Methods:We used western blotting,real-time polymerase chain reaction,and immunohistochemical or immunoflu-orescence to determine DDIT4 expression in GC cells and tissues.High-content screening,cell counting kit-8 assays,colony formation,and in vivo tumorigenesis assays were performed to evaluate cell proliferation.Flow cytometry was used to investigate cell apoptosis and cell cycle distribution.Results:DDIT4 was upregulated in GC cells and tissue.Furthermore,downregulating DDIT4 in GC cells inhibited proliferation both in vitro and in vivo and increased 5-fluorouracil-induced apoptosis and cell cycle arrest.In contrast,ectopic expression of DDIT4 in normal gastric epithelial cells promoted proliferation and attenuated chemosensitivity.Further analysis indicated that the mitogen-activated protein kinase and p53 signaling pathways were involved in the suppression of proliferation,and increased chemosensitivity upon DDIT4 downregulation.Conclusion:DDIT4 promotes GC proliferation and tumorigenesis,providing new insights into the role of DDIT4 in the tumorigenesis of human GC. | Feng Du Lina Sun Yi Chu Tingyu Li Chao Lei Xin Wang Mingzuo Jiang Yali Min Yuanyuan Lu Xiaodi Zhao Yongzhan Nie Daiming Fan | 2018 | Cancer Communications2018，38，1： | 10 |
| 9 | Soil Cellulase Activity and Fungal Community Responses to Wetland Degradation in the Zoige Plateau, China显示文摘Four soil types(peat, marsh, meadow, and sandy) in the Zoige Plateau of China are associated with the severity of wetland degradation. The effects of wetland degradation on the structure and abundance of fungal communities and cellulase activity were assessed in these 4 soil types at 3 depths using DGGE(Denatured Gradient Gel Electrophoresis), q PCR(Quantitative Real-time PCR),and 3,5-dinitrosalicylic acid assays. Cellulase activity and abundance of the fungal community declined in parallel to the level of wetland degradation(from least to most disturbed). DGGE analysis indicated a major shift in composition of fungal communities among the4 soil types consistent with the level of degradation.Water content(WC), organic carbon(OC), total nitrogen(TN), total phosphorus(TP), available nitrogen(AN), and available phosphorus(AP) were strongly correlated with cellulase activity and the structure and abundance of the fungal community.The results indicate that soil physicochemical properties(WC, OC, TN, TP, AN, and AP), cellulase activity, and diversity and abundance of fungal communities are sensitive indicators of the relative level of wetland degradation. WC was the major factorinvolved in Zoige wetland degradation and lower WC levels contributed to declines in the abundance and diversity of the fungal community and reduction in cellulase activity. | WU Li-sha FENG Su NIE Yuan-yang ZHOU Jian-hong YANG Zhi-rong ZHANG Jie | 2015 | Journal of Mountain Science2015，12，2： | 8 |
| 10 | Design of the Metal Precursors Molecular Structures in Impregnating Solutions for Preparation of Efficient Ni Mo/Al_2O_3 Hydrodesulfurization Catalysts显示文摘The molecular structures of metal precursors in the impregnating solution were designed so as to prepare efficient Ni Mo/Al_2O_3 hydrodesulfurization(HDS) catalysts. At first, five typical impregnating solutions were designed; the existing metal precursors, such as [Mo4(citrate)2O11]^(4-)-like, [P2Mo18O62]^(6-)-like and [P2Mo5O23]^(6-)-like species in the solutions were confirmed by laser Raman spectroscopy(LRS). The UV-Vis spectra results indicated that the solutions containing both phosphoric acid and citric acid could change the existing form of nickel species. Five corresponding Ni Mo/Al_2O_3 catalysts were prepared by the incipient wetness impregnation method. The LRS analysis results of dried catalysts showed that the above metal precursors could be partly retained on alumina support after impregnation and drying, although the interface reaction between different metal precursors and alumina support unavoidably took place. Then the catalysts were sulfided and characterized by N2 physisorption, TEM and XPS analyses. The results showed that different metal precursors in impregnating solution could mainly result in the difference in both the morphology of(Ni)Mo S2 slabs and the promoting effect of Ni species. The catalyst prepared mainly with [P2Mo5O23]^(6-)-like species used as precursors exhibited worse dispersion of(Ni)Mo S2 slabs and lower ratio of Ni–Mo–S active phases than the one with [Mo4(citrate)2O11]^(4-)-like species. Promisingly, the catalyst prepared with co-existing [Mo4(citrate)2O11]^(4-)-like, [P2Mo18O62]^(6-)-like and [P2Mo5O23]^(6-)-like species showed better hydrodesulfurization activity for 4,6-DMDBT thanks to its more well-dispersed Ni–Mo–S active phases. | Li Huifeng Li Mingfeng Chu Yang Liu Feng Nie Hong | 2015 | China Petroleum Processing & Petrochemical Technology2015，17，4： | 8 |
| 11 | Fluidized mining and in-situ transformation of deep underground coal resources: a novel approach to ensuring safe, environmentally friendly, low-carbon, and clean utilisation显示文摘Traditional coal mining and utilisation patterns are severely detrimental to natural resources and environments and significantly impede safe, low-carbon, clean, and sustainable utilisation of coal resources. Based on the idea of in situ fluidized coal mining that aims to transform solid coal into liquid or gas and transports the fluidized resources to the ground to ensure safe mining and low-carbon and clean utilisation, in this study, we report on a novel in situ unmanned automatic mining method. This includes a flexible, earthworm-like unmanned automatic mining machine (UAMM) and a coal mine layout for in situ fluidized coal mining suitable for the UAMM. The technological and economic advantages and the carbon emission reduction of the UAMM-based in situ fluidized mining in contrast to traditional mining technologies are evaluated as well. The development trends and possible challenges to this design are also discussed. It is estimated that the proposed method costs approximately 49% of traditional coal mining costs. The UAMM-based in situ fluidized mining and transformation method will reduce CO2 emissions by at least 94.9% compared to traditional coal mining and utilisation methods. The proposed approach is expected to achieve safe and environmentally friendly coal mining as well as lowcarbon and clean utilisation of coal. | Yang Ju Yan Zhu Heping Xie Xiaodong Nie Yong Zhang Chang Lu Feng Gao | 2019 | International Journal of Coal Science & Technology2019，6，2： | 8 |
| 12 | Greenhouse gas emissions and reduction potential of primary aluminum production in China显示文摘A cradle-to-gate life cycle assessment was conducted in this paper to calculate the greenhouse gas (GHG) emissions, such as CO2, CH4, CF4 and C2F6 emissions, based on statistic data of Chinese aluminum industry of the year 2003. The results showed that the GHG emissions for 1 t primary aluminum production was 21.6 t CO2 equivalent which is 70% higher than that of worldwide average level of the year 2000. The main contributors of emission were the alumina refining and aluminum smelting process accounting for 72% and 22% in accumulative emission, respectively. According to the development and application of new process technologies for primary aluminum production and the ‘target of energy-saving and emissions-reducing’ of Chinese government, the reduction potential of the GHG emissions for alumina and aluminum production were estimated. The results indicated that China aluminum industry would achieve the target of reducing about 25% GHG emissions by the end of 2010. | GAO Feng NIE ZuoRen WANG ZhiHong LI HongMei GONG XianZheng ZUO TieYong | 2009 | Science China(Technological Sciences)2009，52，8： | 8 |
| 13 | CIVB flashback analysis of hydrogen flame based on azimuthal vorticity at mixing zone exit显示文摘Swirl-premixed combustion systems exhibit potential to meet future regulations on pollution emissions. However, combustion induced vortex breakdown(CIVB) flashback is frequently observed in these systems, especially for high hydrogen content fuel. In this study, a swirl-premixed burner with diverging centerbody was used to investigate CIVB flashback based on azimuthal vorticity at mixing zone exit. Through 2D axisymmetric model, it was found that there was a maximal azimuthal vorticity at mixing zone exit for each equivalence ratio. The physical meaning of these maximal azimuthal vorticity values was the minimally required azimuthal vorticity to trigger CIVB flashback. At the same time, the required azimuthal vorticity declined with the increase of equivalence ratio since turbulent burning velocity started to control flashback. Nevertheless, azimuthal vorticity offered by heat release increased with the increase of equivalence ratio, which promoted flame propagating upstream continually. | TIAN Xiao Jing XING Shuang Xi CUI Yu Feng FANG Ai Bing NIE Chao Qun | 2014 | Science China(Technological Sciences)2014，57，12： | 7 |
| 14 | An Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens显示文摘Objective To provide a feasible and cost-effective next-generation sequencing(NGS) method for accurate identification of viral pathogens in clinical specimens, because enormous limitations impede the clinical use of common NGS, such as high cost, complicated procedures, tremendous data analysis, and high background noise in clinical samples. Methods Viruses from cell culture materials or clinical specimens were identified following an improved NGS procedure: reduction of background noise by sample preprocessing, viral enrichment by barcoded oligonucleotide(random hexamer or non-ribosomal hexanucleotide) primer-based amplification, fragmentation-free library construction and sequencing of one-tube mixtures, as well as rapid data analysis using an in-house pipeline. Results NGS data demonstrated that both barcoded primer sets were useful to simultaneously capture multiple viral pathogens in cell culture materials or clinical specimens and verified that hexanucleotide primers captured as many viral sequences as hexamers did. Moreover, direct testing of clinical specimens using this improved hexanucleotide primer-based NGS approach provided further detailed genotypes of enteroviruses causing hand, foot, and mouth disease(HFMD) and identified other potential viruses or differentiated misdiagnosis events. Conclusion The improved barcoded oligonucleotide primer-based NGS approach is simplified, time saving, cost effective, and appropriate for direct identification of viral pathogens in clinical practice. | WANG Churl Hua NIE Kai ZHANG Yi WANG Ji ZHOU Shuai Feng LI Xin Na ZHOU Hang Yu QI Shun Xiang MA Xue Jun | 2017 | Biomedical and Environmental Sciences2017，30，1： | 7 |
| 15 | Composite solid electrolyte of Na3PS4-PEO for all-solid-state SnS2/Na batteries with excellent interfacial compatibility between electrolyte and Na metal显示文摘High ionic conductivity and superior interfacial stability of solid electrolytes at the electrodes are crucial factors for high-performance all-solid-state sodium batteries. Herein, a composite solid electrolyte Na3PS4-polyethylene oxide is synthesized by the solution-phase reaction method with an improved ionic conductivity up to 9.4 × 10-5 S/cm at room temperature. Moreover, polyethylene oxide polymer layer is wrapped homogeneously on the surface of Na3PS4 particles, which could effectively avoid the direct contact between Na3PS4 electrolyte and sodium metal, thus alleviate their side reactions. We demonstrate that all-solid-state battery SnS2/Na with the composite solid electrolyte Na3PS4-polyethylene oxide delivers an enhanced electrochemical performance with 230 m Ah/g after 40 cycles. | Xiaoyan Xu Yuanyuan Li Jun Cheng Guangmei Hou Xiangkun Nie Qing Ai Linna Dai Jinkui Feng Lijie Ci | 2020 | Journal of Energy Chemistry2020，29，2： | 7 |
| 16 | Risk factors of prognosis after acute kidney injury in hospitalized patients显示文摘 | Sasa Nie Zhe Feng Lihua Xia Jiuxu Bai Fenglin Xiao Jian Liu Li Tang Xiangmei Chen | 2017 | Frontiers of Medicine2017，11，3： | 7 |
| 17 | Protostemonine effectively attenuates lipopolysaccharide-induced acute lung injury in mice显示文摘Protostemonine (PSN ) 是从 Stemona sessilifolia 的根提取的主要的反煽动性的碱(在繁体中文药作为 Baibu 知道) 。这里,我们报导 lipopolysaccharide (LPS ) 上的 PSN 的禁止的效果在老鼠在 vitro 和导致 LPS 的尖锐的肺损害导致了巨噬细胞激活。巨噬细胞房间线 RAW264.7 房间和老鼠骨头导出髓的巨噬细胞(BMDM ) 为 0.5 h 与 PSN (1, 3, 10, 30 和 100 mol/L ) 被对待然后为 24 h 与 LPS (0.1 g/mL ) 质问了。有 PSN 的预告的处理显著地禁止了 MAPK 和 AKT 的导致 LPS 的 phosphorylation,在巨噬细胞的 iNOS 表示和没有生产。C57BL/6 老鼠 intratracheally 与 LPS (5 mg/kg ) 被注射导致尖锐的肺损害(ALI ) 。老鼠随后与 PSN 被对待(10 mg/kg, ip ) 在在 LPS 以后的 4 和 24 h 挑战。PSN 管理显著地稀释了导致 LPS 的煽动性的房间渗入,减少的支持 inflammatory cytokine (TNF- , IL-1 和 IL-6 ) 生产和消除的调停 LPS 的肺浮肿。而且, PSN 管理显著地禁止了导致 LPS 的肺的军邮局活动。同时,在肺的 p38 MAPK, iNOS 表示和没有生产的导致 LPS 的 phosphorylation 也被压制。结果证明 PSN 有效地在 vitro 并且在 vivo 稀释导致 LPS 的煽动性的回答;有益的效果与 MAPK 和 AKT 和支持 inflammatory 调停人的减少的表示的减少的 phosphorylation 被联系,例如 iNOS,没有并且 cytokines。这些数据建议 PSN 可以是在 ALI 的处理的一个潜在的治疗学的代理人。 | Ya-xian WU Hui-qiong HE Yun-juan NIE Yun-he DING Lei SUN Feng QIAN | 2018 | Acta Pharmacologica Sinica2018，39，1： | 7 |
| 18 | African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2显示文摘African swine fever virus(ASFV),as a member of the large DNA viruses,may regulate autophagy and apoptosis by inhibiting programmed cell death.However,the function of ASFV proteins has not been fully elucidated,especially the role of autophagy in ASFV infection.One of three Pyrroline-5-carboxylate reductases(PYCR),is primarily involved in conversion of glutamate to proline.Previous studies have shown that depletion of PYCR2 was related to the induction of autophagy.In the present study,we found for the first time that ASFV E199 L protein induced a complete autophagy process in Vero and HEK-293 T cells.Through co-immunoprecipitation coupled with mass spectrometry(Co IP-MS)analysis,we firstly identified that E199 L interact with PYCR2 in vitro.Importantly,our work provides evidence that E199 L down-regulated the expression of PYCR2,resulting in autophagy activation.Overall,our results demonstrate that ASFV E199 L protein induces complete autophagy through interaction with PYCR2 and down-regulate the expression level of PYCR2,which provide a valuable reference for the role of autophagy during ASFV infection and contribute to the functional clues of PYCR2. | Sheng Chen Xinheng Zhang Yu Nie Hongxin Li Weiguo Chen Wencheng Lin Feng Chen Qingmei Xie | 2021 | Virologica Sinica2021，36，2： | 7 |
| 19 | Phase I study of CAR-T cells with PD-1 and TCR disruption in mesothelin-positive solid tumors显示文摘Programmed cell death protein-1(PD-1)-mediated immunosuppression has been proposed to contribute to the limited clinical efficacy of chimeric antigen receptor T(CAR-T)cells in solid tumors.We generated PD-1 and T cell receptor(TCR)deficient mesothelin-specific CAR-T(MPTK-CAR-T)cells using CRISPR-Cas9 technology and evaluated them in a dose-escalation study.A total of 15 patients received one or more infusions of MPTK-CAR-T cells without prior lymphodepletion.No dose-limiting toxicity or unexpected adverse events were observed in any of the 15 patients.The best overall response was stable disease(2/15 patients).Circulating MPTK-CAR-T cells peaked at days 7–14 and became undetectable beyond 1 month.TCR-positive CAR-T cells rather than TCR-negative CAR-T cells were predominantly detected in effusion or peripheral blood from three patients after infusion.We further confirmed the reduced persistence of TCR-deficient CAR-T cells in animal models.Our results establish the preliminary feasibility and safety of CRISPR-engineered CAR-T cells with PD-1 disruption and suggest that the natural TCR plays an important role in the persistence of CAR-T cells when treating solid tumors. | Zhenguang Wang Na Li Kaichao Feng Meixia Chen Yan Zhang Yang Liu Qingming Yang Jing Nie Na Tang Xingying Zhang Chen Cheng Lianjun Shen Jiaping He Xun Ye Wei Cao Haoyi Wang Weidong Han | 2021 | Cellular & Molecular Immunology2021，18，9： | 7 |
| 20 | SKP2 siRNA inhibits the degradation of P27^kip1 and down-regulates the expression of MRP in HL-60/A cells显示文摘S 阶段联系 kinase 的蛋白质 2 (SKP2 ) 基因是肿瘤 suppressor 基因,并且涉及 P27kip1 的调停 ubiquitin 的降级。SKP2 和 P27kip1 影响通过调整白血病房间的增长, apoptosis 和区别继续和白血病的预后。在这研究,我们探索了通过 SKP2 下面规定 HL-60/A 药抵抗逆行的机制。HL-60/A 房间是由有 SKP2 siRNA 的 Amaxa Nucleofector 系统的 nucleofected。Skp2, P27kip1,和多药抵抗的基因和蛋白质表示层次联系了蛋白质(MRP ) 被反向的抄写聚合酶链反应和西方的污点分析分别地决定。房间周期被流动 cytometry 分析。50% 禁止的集中价值在 chemotherapeutics 的不同集中下面根据这些二种房间的死亡率用细胞毒素的分析被计算比较房间的敏感。HL-60/A 房间显示出由抗力移转特征的多药抵抗显型到 adriamycin, daunorubicin,和 arabinosylcytosine,由于 MRP 的表示。我们发现 SKP2 的表示比在 HL-60 房间在 HL-60/A 房间是更高的,但是 P27kip1 的表示更低。而 P27kip1 的蛋白质水平是起来调整的,在由 SKP2 siRNA 的 HL-60/A 房间 nucleofected 的 SKP2 的表示是下面调整的。与在控制组(由控制 siRNA 的 nucleofected ) 的 MRP 表达式水平相比, mRNA 和蛋白质表达式由 SKP2 siRNA 在 HL-60/A 房间 nucleofected MRP 铺平更低,并且后者房间对 adriamycin, daunorubicin,和 arabinosylcytosine 更敏感。下面调整在 G0/G1 阶段的 SKP2 表示和逮捕房间与下面调整的 MRP 改进白血病房间的药敏感表示。 | Jie Xiao Songmei Yin Yiqing Li Shuangfeng Xie Danian Nie Liping Ma Xiuju Wang Yudan Wu Jianhong Feng | 2009 | Acta Biochimica et Biophysica Sinica2009，41，8： | 7 |
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