2篇
您的检索式：作者名="Fengqiang Yu"
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| 1 | Coronary heart disease: incidence, risk factors and interventions in Jiaozhou of Shandong province显示文摘 | Yu Hua Li Dan Chu Xianming An Yi Song Tongxun Feng Huixin Lin Peilin Wang Tao Jiang Shaoyan Guo Linlin Xu Fengqiang Liu Zhengke Yang Bin | 2014 | Chinese Medical Journal2014，，12： | 5 |
| 2 | HER4 promotes the growth and metastasis of osteosarcoma via the PI3K/AKT pathway显示文摘Osteosarcoma is the most common primary malignant bone tumor,which occurs in adolescents.As reported by our previous studies,HER4 indicates a poor prognosis of primary osteosarcoma.However,its mechanisms in the pathogenesis of osteosarcoma have not yet been studied.The purpose of this study was to investigate the role of HER4 in osteosarcoma and whether the PI3K/AKT pathway is involved.In this study,western blot analysis was used to investigate the expression of HER4 protein in osteosarcoma tissues and cell lines.CCK8 and transwell assays were used to detect the effects of HER4 on the proliferation,migration,and invasion of osteosarcoma cells in vitro.The effects of HER4 on the growth and metastasis of osteosarcoma in vivo were detected by tumor formation and immunofluorescence in nude mice.The role of the PI3K/AKT pathway in HER4 regulation of the growth and metastasis of osteosarcoma was examined by western blot analysis and immunofluorescence assay.We found that HER4 protein was highly expressed in clinical osteosarcoma specimens and osteosarcoma cells.HER4 markedly promoted the proliferation,migration,and invasion of osteosarcoma cells in vitro as well as the growth and metastasis of osteosarcoma in vivo.HER4 overexpression upregulated the expression of phosphorylated protein kinase B(pAKT),proliferation marker antigen Ki67,and metastasis cell marker matrix metalloproteinase 9(MMP9).Notably,PI3K/AKT inhibitor LY294002 significantly inhibited the effects of HER4 via the downregulation of pAKT,Ki67,and MMP9.Moreover,LY294002 markedly blocked the effects of HER4-induced upregulation of tum or malignancy.The present study suggests that HER4 may promote the growth and metastasis of osteosarcoma via the PI3K/AKT pathway.The HER4/PI3K/AKT pathway could serve as a potential target for the treatment of osteosarcoma. | Xiaodong Li Qingshan Huang Shenglin Wang Zhen Huang Fengqiang Yu Jianhua Lin | 2020 | Acta Biochimica et Biophysica Sinica2020，52，4： | 3 |
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