| 3 | Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system显示文摘BACKGROUND Mesenchymal stem cells(MSCs)represent a promising therapy for the treatment of equine joint diseases,studied due to their possible immunomodulatory characteristics and regenerative capacity.However,the source of most suitable MSCs for producing cartilage for regenerative processes in conjunction with biomaterials for an enhanced function is yet to be established.AIM To compare the chondrogenicity of MSCs derived from synovial fluid,bone marrow,and adipose tissue of horses,using the aggrecan synthesis.METHODS MSCs from ten horses were cultured,phenotypic characterization was done with antibodies CD90,CD44 and CD34 and were differentiated into chondrocytes.The 3D cell culture system in which biocompatible nanoparticles consisting of gold,iron oxide,and poly-L-lysine were added to the cells,and they were forced by magnets to form one microspheroid.The microspheroids were exposed to a commercial culture medium for 4 d,7 d,14 d,and 21 d.Proteoglycan extraction was performed,and aggrecan was quantified by enzyme-linked immunosorbent assay.Keratan sulfate and aggrecan in the microspheroids were identified and localized by immunofluorescence.RESULTS All cultured cells showed fibroblast-like appearance,the ability to adhere to the plastic surface,and were positive for CD44 and CD90,thus confirming the characteristics and morphology of MSCs.The soluble protein concentrations were higher in the microspheroids derived from adipose tissue.The aggrecan concentration and the ratio of aggrecan to soluble proteins were higher in microspheroids derived from synovial fluid than in those derived from bone marrow,thereby showing chondrogenic superiority.Microspheroids from all sources expressed aggrecan and keratan sulfate when observed using confocal immunofluorescence microscopy.All sources of MSCs can synthesize aggrecan,however,MSCs from synovial fluid and adipose tissue have demonstrated better biocompatibility in a 3D environment,thus suggesting chondrogenic superiority.CONCLUSION All sources of MSCs produce hyaline cartilage;however,the use of synovial liquid or adipose tissue should be recommended when it is intended for use with biomaterials or scaffolds. | Joice Fülber Fernanda R Agreste Sarah R T Seidel Eric D P Sotelo Ângela P Barbosa Yara M Michelacci Raquel Y A Baccarin | 2021 | World Journal of Stem Cells2021，13，6： | 0 |
| 4 | Delivery of essential pediatric congenital surgical care within Brazil’s universal health coverage system:a national survey of pediatric surgeons显示文摘Objective In this study,we assess the delivery of congenital pediatric surgical care under Brazil’s system of universal health coverage and evaluate differences in delivery between public and private sectors.Methods A cross-sectional national survey of pediatric surgeons in Brazil was conducted.Participants were asked which of 23 interventions identified through the Disease Control Priorities 3(Surgical Interventions for Congenital Anomalies)they perform and to report barriers faced while providing surgical care.Responses were weighted by state and stratified by sector(public vs private).Results A sample of 352 responses was obtained and weighted to represent 1378 practicing pediatric surgeons registered in Brazil during the survey time.73%spend the majority of their time working in the public sector(‘SistemaÚnico de Saúde’and Foundation hospitals),and most of them also work in the private sector.Generally,Brazilian pediatric surgeons have the expertise to provide thoracic,abdominal,and urologic procedures.Surgeons working mostly in the public sector were more likely to report a lack of access to essential medications(25%vs 9%,p<0.01)and a lack of access to hospital beds for surgical patients(52%vs 32%,p<0.01).Conclusions Brazilian pediatric surgeons routinely perform thoracic,abdominal,and urologic surgery.Those working in government-financed hospitals face barriers related to infrastructure,which may impact Brazilians who rely on Brazil’s universal health coverage system.Policies that support pediatric surgeons working in the public sector may promote the workforce available to provide congenital pediatric surgical care. | Paul R Truche Abbie E Naus Fabio Botelho Julia Ferreira Alexis Bowder Luke Caddell Kathrin Zimmerman Isabella Maria de Freitas Faria Bellisa Caldas Lopes Eduardo Corrêa Costa Fernanda Lage Lima Dantas Augusto J S A Cavalcante Carlos A L B Carvalho Simone Abib David P Mooney Nivaldo Alonso | 2023 | World Journal of Pediatric Surgery2023，6，3： | 0 |
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