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| 1 | Glutamine prevents oxidative stress in a model of mesenteric ischemia and reperfusion显示文摘AIM: To evaluate preventative effects of glutamine in an animal model of gut ischemia/reperfusion(I/R).METHODS: Male Wistar rats were housed in a controlled environment and allowed access to food and water ad libitum. Twenty male Wistar rats were divided into four experimental groups:(1) control group(control)- rats underwent exploratory laparotomy;(2) control + glutamine group(control-GLU)- rats were subjected to laparotomy and treated intraperitoneally with glutamine 24 and 48 h prior to surgery;(3) I/R group- rats were subjected to occlusion of the superior mesenteric artery for 30 min followed by 15 min of reperfusion; and(4) ischemia/reperfusion + glutamine group(G + I/R)- rats were treated intraperitoneally with glutamine 24 and 48 h before I/R. Local and systemic injuries were determined by evaluating intestinal and lung segments for oxidative stress using lipid peroxidation and the activity of superoxide dismutase(SOD), interleukin-6(IL-6) and nuclear factor kappa beta(NFkB) after mesenteric I/R. RESULTS: Lipid peroxidation of the membrane was increased in the animals subjected to I/R(P < 0.05). However, the group that received glutamine 24 and 48 h before the I/R procedure showed levels of lipid peroxidation similar to the control groups(P < 0.05). The activity of the antioxidant enzyme SOD was decreased in the gut of animals subjected to I/R when compared with the control group of animals not subjected to I/R(P < 0.05). However, the group that received glutamine 24 and 48 h before I/R showed similar SOD activity to both control groups not subjected to I/R(P < 0.05). The mean area of NF-kB staining for each of the control groups was similar. The I/R group showed the largest area of staining for NF-kB. The G + I/R group had the second highest amount of staining, but the mean value was much lower than that of the I/R group(P < 0.05). For IL-6, control and control-GLU groups showed similar areas of staining. The I/R group contained the largest area of IL-6 staining, followed by the G + I/R animals; however, this area was significantly lower than that of the group that underwent I/R without glutamine(P < 0.05). CONCLUSION: These results demonstrate that pretreatment with glutamine prevents mucosal injury and improves gut and lung recovery after I/R injury in rats. | Gilmara Pandolfo Zabot Gustavo Franco Carvalhal Norma Possa Marroni Renata Minuzzo Hartmann Vinícius Duval da Silva Henrique Sarubbi Fillmann | 2014 | World Journal of Gastroenterology2014，20，32： | 7 |
| 2 | Characterization of cytotoxic compound from mangrove derived fungi Irpex hydnoides VB4显示文摘Objective:To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.Methods:In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers.Blast search sequence similarity was found against the existing non redundant nucleotide sequence database thus,identified as Aspergilus flavus,Hyporcaea lixii,Aspergillus niger,Eutorium amstelodami,Irpex hydnoides and Neurospora crassa.Among the seven isolates, one fungi Irpex hydnoides was selected for further studies.The fungi were grown in sabouraud broth for five days and filtrate were separated and subjected to ethyl acetate for further studies. Results:Nearly half(49.25%) of the extracts showed activity(IC_(50) of 125 μ g/mL).These values were within the cutoff point of the National Cancer Institute criteria for cytotoxicity(IC_(50)<20 μ g/mL) in the screening of crude plant extracts.The GC MS analysis revealed that the active principals might be Tetradecane(6.26%) with the RT 8.606.Conclusions:It is clear from the present study that mangrove fungi with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical,anti cancer screening programmes.The results help us conclude that me potential of using metabolic engineering and post genomic approaches to isolate more novel bioactive compounds and to make their possible commercial application is not far off. | B Valentin Bhimba DA Agnel Defora Franco Geena Mary Jose Jibi Merin Mathew Elsa Lycias Joel | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，3： | 3 |
| 3 | Glutamine prevents oxidative stress in a model of portal hypertension显示文摘AIM To evaluate the protective effects of glutamine in a model of portal hypertension(PH) induced by partial portal vein ligation(PPVL).METHODS Male Wistar rats were housed in a controlled environment and were allowed access to food and water ad libitum. Twenty-four male Wistar rats were divided into four experimental groups:(1) control group(SO)-rats underwent exploratory laparotomy;(2) control + glutamine group(SO + G)-rats were subjected to laparotomy and were treated intraperitoneally with glutamine;(3) portal hypertension group(PPVL)-rats were subjected to PPVL; and(4) PPVL + glutamine group(PPVL + G)-rats were treated intraperitoneally with glutamine for seven days. Local injuries were determined by evaluating intestinal segments for oxidative stress using lipid peroxidation and the activities of glutathione peroxidase(GPx), endothelial nitric oxide synthase(e NOS) and inducible nitric oxide synthase(i NOS) after PPVL.RESULTS Lipid peroxidation of the membrane was increased in the animals subjected to PH(P < 0.01). However, the group that received glutamine for seven days after the PPVL procedure showed levels of lipid peroxidation similar to those of the control groups(P > 0.05). The activity of the antioxidant enzyme GTx was decreased in the gut of animals subjected to PH compared with that in the control group of animals not subjected to PH(P < 0.01). However, the group that received glutamine for seven days after the PPVL showed similar GTx activity to both the control groups not subjected to PH(P > 0.05). At least 10 random, non-overlapping images of each histological slide with 200 × magnification(44 pixel = 1 μm) were captured. The sum means of all áreas, of each group were calculated. The mean areas of e NOS staining for both of the control groups were similar. The PPVL group showed the largest area of staining for e NOS. The PPVL + G group had the second highest amount of staining, but the mean value was much lower than that of the PPVL group(P < 0.01). For i NOS, the control(SO) and control + G(SO + G) groups showed similar areas of staining. The PPVL group contained the largest area of i NOS staining, followed by the PPVL + G group; however, this area was significantly smaller than that of the group that underwent PH without glutamine(P < 0.01).CONCLUSION Treatment with glutamine prevents gut mucosal injury after PH in rats. | Gilmara Pandolfo Zabot Gustavo Franco Carvalhal Norma Possa Marroni Francielli Licks Renata Minuzzo Hartmann Vinícius Duval da Silva Henrique Sarubbi Fillmann | 2017 | World Journal of Gastroenterology2017，23，25： | 3 |
| 4 | Structure of NiCrAlY coatings deposited on single-crystal alloy turbine blade material by laser cladding 显示文摘 | VILAR R SANTOS E C FERREIRA P N FRANCO N DA SILVA | 2009 | Acta Materialia2009，57，18： | 1 |
| 5 | Assessment of aroma impact compounds in a cashew apple-based alcoholic beverage by GC-MS and GC-olfactometry显示文摘 | GARRUTI D S FRANCO M R B da SILVA M A | 2006 | LWT-Food Science and Technology2006，39，4： | 1 |
| 6 | Effect of bacterial lipopolysaccharide on isehemic damage in the rat retina 显示文摘 | Franco PJ Fernandez DC Sande PH Keller-Sarmiento MI Chianelli M Saenz DA | 2008 | Invest Ophthalmol Vis Sci2008，49，10： | 1 |
| 7 | Resveratrol prevents hyperleptinemia and central leptin resistance in adult rats programmed by early weaning显示文摘 | Franco JG Lisboa PC da Silva Lima N | 2014 | Horm Metab Res2014，46，10： | 1 |
| 8 | Old game, new players: Linking classical theories to new trends in transplant immunology显示文摘The evolutionary emergence of an efficient immune system has a fundamental role in our survival against pathogenic attacks. Nevertheless, this same protective mechanism may also establish a negative consequence in the setting of disorders such as autoimmunity and transplant rejection. In light of the latter, although research has long uncovered main concepts of allogeneic recognition, immune rejection is still the main obstacle to long-term graft survival. Therefore, in order to define effective therapies that prolong graft viability, it is essential that we understand the underlying mediators and mechanisms that participate in transplant rejection. This multifaceted process is characterized by diverse cellular and humoral participants with innate and adaptive functions that can determine the type of rejection or promote graft acceptance. Although a number of mediators of graft recognition have been described in traditional immunology, recent studies indicate that defining rigid roles for certain immune cells and factors may be more complicated than originally conceived. Current research has also targeted specific cells and drugs that regulate immune activation and induce tolerance. This review will give a broad view of the most recent understanding of the allogeneic inflammatory/tolerogenic response and current insights into cellular and drug therapies that modulate immune activation that may prove to be useful in the induction of tolerance in the clinical setting. | Marina Burgos da Silva Flavia Franco da Cunha Fernanda Fernandes Terra Niels Olsen Saraiva Camara | 2017 | World Journal of Transplantation2017，7，1： | 1 |
| 9 | 5-FU muhifocal inflammatory leu koencephalopathy and dihydropyrimidine dehydrogense deft- ciency显示文摘 | Franco DA Greenberg HS | 2001 | Neurology2001，56，11： | 1 |
| 10 | Addition of propolis or monensin in the diet: Behavior and productivity of lambs in feedlot显示文摘 | C.C.B.F. ítavo M.G. Morais C. Costa L.C.V. ítavo G.L. Franco J.A. da Silva F.A. Reis | 2011 | Animal Feed Science and Technology2011，，3： | 1 |
| 11 | Surface, kinetics and electrocatalytic properties of Ti/(IrO 2 + Ta 2 O 5 ) electrodes, prepared using controlled cooling rate, for ozone production显示文摘 | Leonardo M. Da Silva Débora V. Franco Luiz A. De Faria Julien F.C. Boodts | 2004 | Electrochimica Acta2004，，22： | 1 |
| 12 | Epitope affinity for MHC class I determines helper requirement for CTL priming显示文摘 | Franco A Tilly DA Gramaglia I | 2000 | Nat Immunol2000，1，2： | 1 |
| 13 | Quantitative determination of the phenolic antioxidants using voltammetric techniques显示文摘 | DOS SANTOS RAYMUNDOA M DA SILVA PAULAB M M FRANCO C | 2007 | LWT-Food Science and Technology2007，40，7： | 1 |
| 14 | Nickel(11) and zinc(11) removal using Amberlite IR-120 resin: Ion exchange equilibrium and kinetics 显示文摘 | FRANCO P E VEIT M T BORBA C E GONCALVES G D FAGUNDES-KLEN M R BERGAMASCO R da SILVA E D SUZAKI P Y R | 2013 | Chemical Engineering Journal2013，221，： | 1 |
| 15 | Evaluation of vertical transmission of Toxoplasma gondii in Calomys callosus model after reinfection with heterologous and virulent strain显示文摘 | Franco PS Silva DA Costam IN | 2010 | Placenta2010，36，8： | 1 |
| 16 | Characterisation of Corrosion Products in Cr Implanted Mg Surfaces显示文摘 | Vilarigues M Alves L C Nogueira I D Franco N Sequeira A D da Silva R C | 2002 | Surface and Coatings Technology2002，，： | 1 |
| 17 | Peroxisome proliferator-activated receptors alpha and gamma2 polymorphisms in nonalcoholic fatty liver disease: A study in Brazilian patients显示文摘 | Fernanda Aparecida Domenici Maria José Franco Brochado Ana de Lourdes Candolo Martinelli Sergio Zucoloto Selma Freire de Carvalho da Cunha Helio Vannucchi | 2013 | Gene2013，，： | 1 |
| 18 | Nickel(II) and zinc(II) removal using Amberlite IR-120 resin: Ion exchange equilibrium and kinetics显示文摘 | Pietro Escobar Franco Márcia Teresinha Veit Carlos Eduardo Borba Gilberto da Cunha Gon?alves Márcia Regina Fagundes-Klen Rosangela Bergamasco Edson Antonio da Silva Pedro Yahico Ramos Suzaki | 2013 | Chemical Engineering Journal2013，，： | 1 |
| 19 | Fracture resistance of endodontically trea- ted teeth restored with glass fiber posts of different lengths显示文摘 | Franco E B Lins do Valle A Pomp6ia Fraga de Almei- da A L | 2014 | J Prosthet Dent2014，111，1： | 1 |
| 20 | Ferromagnetism induced in rutile single crystals by argon and nitrogen im- plantation显示文摘 | Cruz M M da Silva R C Franco N | 2009 | J Phys: Condens Matter2009，21，20： | 1 |
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