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| 1 | IL-10 polymorphism is associated with increased incidence of severe sepsis显示文摘Objective To investigate whether three biallelic polymorphisms at positions -592, -819 and -1082 in the promoter region of the IL-10 gene are associated with increased incidence of severe sepsis.Methods The IL-10 -592, -819 and -1082 polymorphisms were typed using polymerase chain reaction followed by digestion with the restriction enzymes RsaⅠ, MaeⅢ and MnlⅠ, respectively.Results Patients with severe sepsis were more likely to have IL-10 -1082 allele 1, compared with controls (P<0.05). Genotype distribution of the IL-10 -1082 polymorphism significantly differed between patients and controls (P<0.05). However, the allele frequencies and genotype distribution of the IL-10 -1082 polymorphism did not differ between surviving and dead patients (P>0.05). No significant differences in the genotype distribution and allele frequencies of the IL-10 -592 and IL-10 -819 polymorphisms were observed between patients with severe sepsis and heathy controls, nor between surviving and dead patients (P> 0.05). Conclusions The polymorphism at position -1082 in the promoter region of the IL-10 gene may be associated with susceptibility to severe sepsis. In constrast, the other two highly linked IL-10 polymorphisms are not associated with incidence or the outcome of severe sepsis. | 舒强 方向明 陈齐兴 Frank Stuber | 2003 | Chinese Medical Journal2003，，11： | 6 |
| 2 | A genomic polymorphism within the tumor necrosis factor locus influences plasma TNFα concentrations and outcome of patients with severe sepsis显示文摘 | Stuber F Peterson M Frank B | 1996 | Crit Care Med1996，24，： | 1 |
| 3 | Supported Cu(II) polymer catalysts for aqueous phenol oxidation显示文摘 | Castro Isabel U Stuber Frank Fabregat Azael | 2009 | Journal of Hazardous Materials2009，163，2： | 1 |
| 4 | Local measurements for the study of external loop airlift hydrodynamics显示文摘 | Marianne Utiger Frank Stuber Duquenne A M | 1999 | Canadian Journal of Chemical Engineering1999，77，4： | 1 |
| 5 | 白细胞介素-10对人外周血白细胞β-防御素2基因诱导性表达的影响显示文摘目的:探讨炎症抑制因子白细胞介素-10(白介素-10)在β-防御素2(hBD-2)基因诱导性表达中的作用。方法:22例健康人被纳入本研究中,以终浓度0 ng/m l的LPS、100 ng/m l的LPS、10ng/m l的IL-10、100 ng/m l的LPS+10 ng/m l的IL-10加入900μl人外周血中,于37℃刺激培养6 h后,提取外周血白细胞总RNA,用实时定量RT-PCR的方法测定外周血白细胞hBD-2mRNA的表达水平。结果:0 ng/m l的LPS或10 ng/m l的IL-10刺激6 h的白细胞中未检测到hBD-2 mRNA,100 ng/m l的LPS刺激6 h后hBD-2mRNA的表达水平为166.9±35.14,而IL-10与LPS共同刺激6 h后hBD-2 mRNA的表达水平为30.40±9.18。IL-10使hBD-2mRNA的诱导性表达水平明显降低(P<0.05)。结论:人外周血白细胞中hBD-2基因呈现诱导性表达,IL-10能下调hBD-2的诱导性表达。 | 陈齐兴 周景琳 Malte Book Frank Stuber 方向明 | 2006 | 浙江大学学报(医学版)2006，35，6： | 1 |
| 6 | Catalytic wet air oxidation of substituted phenols using activated carbon as catalyst显示文摘 | M Eugenia Suarez-Ojeda Frank Stuber Agust Fortuny | 2005 | Applied Catalysis B:Environmental(58)2005，，： | 1 |
| 7 | Supported Cu(Ⅱ) polymer catalysts for aqueous phenol oxidation显示文摘 | Castro Isabel U Stuber Frank Fabregat Azael | 2009 | Journal of Hazardous Materials2009，163，23： | 1 |
| 8 | Interleukin 10.G microsatellite in the promoter region of the interleukin-10 gene in severe sepsis显示文摘Background The highly polymorphic interleukin 10.G (IL10.G) microsatellite located in the promoter region of the interleukin-10 (IL-10) gene exerts a positive transcriptional regulatory effect on IL-10 gene expression and correlates with the in vitro IL-10 secretion. This study was conducted to investigate whether IL10.G microsatellite is associated with the incidence and /or the outcome of severe sepsis. Methods One hundred and fifteen patients with severe sepsis who had been treated at the intensive care unit of the university hospital were studied. One hundred and forty-one healthy individuals served as controls. IL10.G microsatellite genotyping was performed with the following two methods: fluorescent based polymerase chain reaction (PCR) techniques and silver staining of the amplified DNA fragment in polyacrylamide gel. Alleles were defined according to the size of the amplified DNA product. Results Ten alleles and 36 genotypes were detected both in the patients with severe sepsis and in the healthy controls. Allele IL10.G9 and allele IL10.G13 were the commonest alleles with the frequencies of 32.6% and 21.3% respectively in the patients with severe sepsis, and 34% and 27% respectively in the healthy controls. The allele frequencies of IL10.G microsatellite were neither different between the patients with severe sepsis and the healthy controls (P > 0.05), nor between survivors and non-survivors (P > 0.05). However, the frequency of one common allele IL10.G13 was slightly lower in the patients with severe sepsis than in the healthy controls (21.3% vs 27%, P > 0.05), and the frequency of allele IL10.G9 was slightly higher in the non-survivors than in the survivors (37.1% vs 28.1%, P > 0.05). Conclusion IL10.G microsatellite may neither contribute to the susceptibility to severe sepsis nor to the fataloutcome of severe sepsis. | SHU Qiang SHI Chang-chun ZHANG Xiang-hong SHI Zhuo SHI Shan-shan FANG Xiang-ming CHEN Qi-xing Stuber Frank | 2006 | Chinese Medical Journal2006，，3： | 0 |
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