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| 1 | Regulation of actin cytoskeleton via photolithographic micropatterning显示文摘Actin cytoskeleton plays crucial roles in various cellular functions.Extracellular matrix(ECM)can modulate cell morphology by remodeling the internal cytoskeleton.To define how geometry of ECM regulates the organization of actin cytoskeleton,we plated individual NIH 3T3 cells on micropatterned substrates with distinct shapes and sizes.It was found that the stress fibers could form along the nonadhesive edges of T-shaped pattern,but were absent from the opening edge of V-shaped pattern,indicating that the organization of actin cytoskeleton was dependent on the mechanical environment.Furthermore,a secondary actin ring was observed on 50μm circular pattern while did not appear on 30μm and 40μm pattern,showing a size-dependent organization of actin cytoskeleton.Finally,osteoblasts,MDCK and A549 cells exhibited distinct organization of actin cytoskeleton on T-shaped pattern,suggesting a cell-type specificity in arrangement of actin cytoskeleton.Together,our findings brought novel insight into the organization of actin cytoskeleton on micropatterned environments. | Fulin Xing Haimei Zhang Mengyu Li Hao Dong Xuehe Ma Shiyu Deng Fen Hu Imshik Lee Leiting Pan Jingjun Xu | 2023 | Journal of Innovative Optical Health Sciences2023，16，2： | 1 |
| 2 | Single-cell manipulation by two-dimensional micropatterning显示文摘Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape,cytoskeleton and organelle,as well as the nucleus morphology and genetic expression.The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis,cellular standardization,and in vivo environment mimicking.Here,we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques,including photolithographic micropatterning and soft lithography micropatterning.Moreover,we summarize the application of micropatterning technique in controlling cytoskeleton,cell migration,nucleus and gene expression,as well as intercellular communication. | Xuehe Ma Haimei Zhang Shiyu Deng Qiushuo Sun Qingsong Hu Yuhang Pan Fen Hu Imshik Lee Fulin Xing Leiting Pan | 2024 | Journal of Innovative Optical Health Sciences2024，17，1： | 0 |
| 3 | Structural and functional studies of erythrocyte membrane-skeleton by single-cell and single-molecule techniques显示文摘As the indispensable oxygen-transporting cells,erythrocytes exhibit extreme deformability and amazing stability as they are subject to huge reversible shear stress and extrusion force during massive circulation in the body.The unique architecture of spectrin-actin-based membraneskeleton is considered to be responsible for such excellent mechanical properties of erythrocytes.Although erythrocytes have been recognized for more than 300 years,myriad questions about membrane-skeleton constantly attract people's attention.Here,we summarize the kinds of distinctive single-cell and single-molecule techniques that were used to investigate the structure and function of erythrocyte membrane-skeleton at macro and micro levels. | Fulin Xing Fen Hu Jianyu Yang Leiting Pan Jingjun Xu | 2019 | Journal of Innovative Optical Health Sciences2019，，1： | 0 |
| 4 | Regulation of intracellular Ca^2+/CaMKII signaling by TRPV4 membrane translocation during osteoblastic differentiation显示文摘Bone constantly remodels between resorption by osteoclasts and formation by osteoblasts;therefore the functions of osteoblasts are pivotal for maintaining homeostasis of bone mass.Transient receptor potential vanilloid 4(TRPV4),a type of mechanosensitive channel,has been reported to be a key regulator in bone remodeling.Howevei;the relationship between TRPV4 and osteoblast function remains largely elusive.Only little is known about the spatial distribution change of TRPV4 during osteoblastic differentiation and related signal events.Based on three-dimensional super-resolution microscopy,our results clearly showed a different distribution of TRPV4 in undifferentiated and differentiated osteoblasts,which reflected the plasma membrane translocation of TRPV4 along with prolonged differentiation.GSK1016790A(GSK101),the most potent agonist of TRPV4,triggered rapid calcium entry and calmodulin-dependent protein kinase II(CaMKII)phosphorylation via TRPV4 acti・vation in a differentiation-dependent manner,indicating that the abundance of TRPV4 at the cell surface resulting from differentiation may be related to the modulation of Ca2+response and CaMKII activity.These data provide compelling evidences for the plasma membrane translocation of TRPV4 during osteoblastic differentiation as well as demonstrate the regulation of downstream Ca2+/CaMKII signaling. | Fen Hu Yali Zhao Zhenhai Hui Fulin Xing Jianyu Yang Imshik Lee Xinzheng Zhang Leiting Pan Jingjun Xu | 2019 | Biophysics Reports2019，5，5： | 0 |
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