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1篇 您的检索式：作者名="Fuying Kang"

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		题名	作者	年代	出处	被引量
	1	Significant expansion and red-shifting of fluorescent protein chromophore determined through computational design and genetic code expansion显示文摘Fluorescent proteins (FPs)with emission wavelengths in the far-red and infrared regions of the spectrum provide powerful tools for deep-tissue and super-resolution imaging.The development of red-shifted FPs has evoked widespread interest and continuous engineering efforts.In this article, based on a computational design and genetic code expansion,we report a rational approach to significantly expand and red-shift the chromophore of green fluorescent protein (GFP).We applied computational calculations to predict the excitation and emission wavelengths of a FP chromophore harboring unnatural amino acids (UAA)and identify in silico an appropriate UAA,2-amino-3-(6- hydroxynaphthalen-2-yl)propanoic acid (naphthol-Ala).Our methodology allowed us to formulate a GFP variant (cpsfGFP-66-Naphthol-Ala)with red-shifted absorbance and emission spectral maxima exceeding 60 and 130 nm,respectively,compared to those of GFP.The GFP chromophore is formed through autocatalytic post-translational modification to generate a planar 4-(p-hydroxybenzylidene)-5- imidazolinone chromophore.We solved the crystal structure of cpsfGFP-66-naphthol-Ala at 1.3 ■ resolution and demonstrated the formation of a much larger conjugated n-system when the phenol group is replaced by naphthol.These results explain the significant red-shifting of the excitation and emission spectra of cpsfGFP-66-naphthol-Ala.	Li Wang Xian Chen Xuzhen GUO liasong Li Qi Liu Fuying Kang Xudong Wang Cheng HU Haiping Liu Weimin Gong Wei Zhuang Xiaohong Liu Jiangyun Wang	2018	Biophysics Reports2018，4，5：	0

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