| 1 | 棉铃虫幼虫脑和咽下神经节的三维结构构建显示文摘【目的】解剖棉铃虫Helicoverpa armigera(Hübner)5龄幼虫脑和咽下神经节及其内部神经髓形态结构,并分析和构建幼虫脑和咽下神经节以及各神经髓的三维结构模型。【方法】采用免疫组织化学方法解剖脑和咽下神经节的内部神经髓结构,利用激光共聚焦显微镜获取脑和咽下神经节扫描图像,然后利用AMIRA三维图像分析软件进行图像分析,从而构建脑和咽下神经节的三维结构模型,并测量脑和咽下神经节以及内部各神经髓的体积,并分析了相对比例。【结果】棉铃虫5龄幼虫脑和咽下神经节由围咽神经索连接在一起。脑主要由前脑、中脑和后脑3部分组成。前脑内包括视叶、蕈形体和中央体等形态结构较明显的神经髓。此外,前脑还包括其他位于脑的左右两侧以及背侧和腹侧大量神经髓区域,约占脑总神经髓的59.65%。这些神经髓区域边界不明显。中脑主要包括1对触角叶;后脑位于脑的腹侧和触角叶的下方,体积较小。咽下神经节由3个神经节融合构成,从前到后分别为上颚神经节、下颚神经节和下唇神经节,由于融合的紧密程度高,3个神经节间的边界不明显。【结论】阐明了棉铃虫5龄幼虫脑和咽下神经节的神经髓形态结构,构建了脑和咽下神经节以及内部神经髓的三维结构模型。三维模型可以任意旋转,能从任何角度观察脑、咽下神经节和内部不同神经髓的结构及其它们之间的空间关系。本研究结果对研究棉铃虫脑和咽下神经节信息接收、处理及调控行为的机制奠定了解剖学基础。 | 汤清波 詹欢 Bente G.BERG 闫凤鸣 赵新成 | 2014 | 昆虫学报2014，57，5： | 9 |
| 3 | Neuronal architecture and functional mapping of the taste center of larval Helicoverpa armigera(Lepidoptera:Noctuidae)显示文摘The sense of taste plays a crucial role in herbivorous insects by discriminating nutrients from complex plant metabolic compounds.The peripheral coding of taste has been thoroughly studied in many insect species,but the central gustatory pathways are poorly described.In the present study,we characterized single neurons in the gnathal ganglion of Helicoverpa armigera larvae using the intracellular recording/staining technique.We identified different types of neurons,including sensory neurons,interneurons,and motor neurons.The morphologies of these neurons were largely diverse and their arborizations seemingly covered the whole gnathal ganglion.The representation of the single neurons responding to the relevant stimuli of sweet and bitter cues showed no distinct patterns in the gnathal ganglion.We postulate that taste signals may be processed in a manner consistent with the principle of population coding in the gnathal ganglion of H.armigera larvae. | Long-Long Sun Xiao-Lan Liu Ya-Nan Wang Bente G.Berg Gui-Ying Xie Wen-Bo Chen Yang Liu Gui-Rong Wang Xin-Cheng Zhao Qing-Bo Tang | 2022 | Insect Science2022，29，3： | 0 |
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