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| 1 | 银鲫IgM的纯化及其单克隆抗体的制备显示文摘Aeromonas hydrophila septicemia was a serious infectious disease occurred in China.The majority harmed fish was crucian carp(Carassius auratus gibelio).It was reported that immune prevention was the effect measure to prevent this disease using A.hydrophila bacterin.So it is necessary to develop monoclonal antibody(McAb) against the IgM from Crucian carp.In this study,the purification of serum immunoglobulin from Crucian carp was carried out by using Goat IgG Agarose.The concentration of purified IgM was 2.5 mg·mL-1.By using hybridoma-monoclonal antibody technology,hybridoma cell line secreted McAb against the IgM from crucian carp had been established and the characteristics of mab8 had been described.In isotyping analysis,the results showed that it belong to IgG1,it was able to recognize the heavy chain of the IgM on western blot,and did not has any cross reaction with the Ig from 19 different fish species including common carp(Cyprinus carpio),bighead carp(Aristichthys nobilis),grass carp(Ctenopharhyngodon idella) and bream(Abramis brama) etc.The results demonstrated that McAb was highly specific and sensitive to the Ig of Crucian carp,and had the great potential to be used for analyzing the structure of crucian carp IgM,monitoring the immunity level of cultured Crucian carp and pathogen diagnosis. | 沈锦玉 Thompson K D Adams A Galina Jeney 钱冬 沈智华 | 2006 | 水产学报2006,30,3: | 4 |
| 2 | Panax ginseng natural populations: their past, current state and perspectives显示文摘瞄准:人参属人参,遗传和它 Primorye (俄国) 的自然人口的个体发生的结构的交配系统被调查。方法:基因差异用紧密相联的酶和荧光灯地基于的自动化放大碎片长度多型性(AFLP ) 和简单顺序被估计重复(SSR ) 标记。结果:在种类水平的全部的基因差异与紧密相联的酶是低的试金(0.023 ) ,和有 AFLP (0.255 ) 和 SSR (0.259 ) 的高度方法。它根据紧密相联的酶(>99%) 在人口以内被观察, AFLP (>85%) ,和 SSR (>73%) 试金。基因可变性分发的索引指出开拓殖民地在早 Holocene 在温暖的时期期间从南部的避难处由人参的 Sikhote-Alin 种。到异花受粉和自花传粉的人参植物的能力被显示出,人参属人参是容许的单性生殖的植物的假设被证实。人参的繁殖系统拥有授精进程的高粘性和稳定性帮助种类处于压力条件幸存。外部或内部的因素引起的骚乱能由于人参小卵或单性生殖的胚胎开发的形态基因的潜力被减少。因为一些生活阶段是不在的或很少发生,人参人口的生活阶段结构的分析证明所有他们不是完整成分的。结论:在所有 3 张人口,处女和年轻生产个人是占优势的。人口学习了的这个工具是可行的,自然人参人口的再介绍还是可能的。 | Yuri N ZHURAVLEV Olga G KOREN Galina D REUNOVA Tamara I MUZAROK Tatiyana Yu GORPENCHENKO Irina L KATS Yuliya A KHROLENKO | 2008 | Acta Pharmacologica Sinica2008,29,9: | 3 |
| 3 | Unique Marine Olenekian-Anisian Boundary Section from South Primorye, Russian Far East显示文摘To show paleontological characteristics of the Olenekian-Anisian boundary beds in the Russian Far East, a review of new data on the Upper Olenekian and Lower Anisian biostratigraphy of South Primorye is given on the basis of five sections: Golyi Cape, Petrovka River, Zhitkov Peninsula, Tchernyschew Bay and Atlasov Cape, using new ammonoid, brachiopod and conodont findings. The most representative ammonoid assemblage at the base of the Anisian was discovered in the Ussuriphyllites amurensis Zone (10.6 m thick) of the Atlasov Cape Section: Parasageceras sp. nov., Prionitidae gen. et sp. nov., Ussuriphyllites amurensis (Kiparisova) (dominant), Megaphyllites atlasoviensis Zakharov, Leiophyllites praematurus Kiparisova, Leiophyllites sp., Ussurites sp., Paradanubites sp. indet., Paracrochordiceras sp. nov., Prohungarites popowi Kiparisova, Arctohungarites primoriensis Zakharov, A. solimani (Toula), Salterites sp. indet. (gigantic shell), and Tropigastrites sublachontanus Zakharov. Conodonts Neospathodus cf. homeri (Bender) were found in the lower part of the Ussuriphyllites amurensis Zone of the Atlasov Cape. The Atlasov Cape seems to be one of the very promising sections of the Russian Far East for detailed investigation of the Olenekian-Anisian boundary. | Yuri D Zakharov Alexander M Popov Galina I Buryi | 2005 | Journal of China University of Geosciences2005,16,3: | 3 |
| 4 | Structure and Deformational Behavior of Poly(vinylidene fluoride) Hard Elastic Films显示文摘 | Ivan D Viii B Viktor L Galina E | 2007 | Acta chimica slovenica2007,54,: | 1 |
| 5 | First experience with near real-time water vapor estimation in a German GPS nertwork 显示文摘 | Galina D Gerd G and Christoph R | 2001 | Journal of Atmospheric and Solar - Terrestial Physics2001,63,: | 1 |
| 6 | Development of a GNSS Water Vapour Tomography System Using Algebraic Reconstruction Techniques 显示文摘 | BENDER M GALINA D GE Maorong | 2011 | Advances in Space Research2011,47,10: | 1 |
| 7 | Z-bus loss allocation显示文摘 | Conejo A J Galina F D | 2001 | IEEE Trans on PWRS2001,16,1: | 1 |
| 8 | Fattening pelibuey lambs with sugar cane tops and corn complemented with or without slow intake urea supplement 显示文摘 | Galina M A Guerrero M PUGA C D | 2007 | Small Ruminant Research2007,70,2: | 1 |
| 9 | Effect of a con- trolled release urea supplement on rumen fermentation in sheepfed a diet of sugar cane tops (Saccharum officinarum ), corn stubble ( Zea ma ys ) and King grass ( Pennisetum purpureum ) 显示文摘 | Puga D C Galina H M Perez-Gil R F etal | 2001 | Small Ruminant Research2001,39,3: | 1 |
| 10 | Effect of a controlled-release urea supplementation onfeed intake,digestibility,nitrogen balance and ruminalkinetics of sheep fed low quality tropical forage 显示文摘 | PUGA D C GALINA H M PEREZ-GIL R F | 2001 | Small Ruminant Research2001,41,1: | 1 |
| 11 | Networks and geography: Modelling community network structures as the outcome of both spatial and network processes 显示文摘 | ] Galina D etc | 2012 | Social Networks2012,34,1: | 1 |
| 12 | An electricity market with a probabilistic spinning reserve criterion显示文摘 | Bouffard F Galina F D | 2004 | IEEE Transactions on Power System2004,19,1: | 1 |
| 13 | Rapid and efficient eleetroporation-based gene transfer into primary dissociated neurons 显示文摘 | Galina D Martin H Corinna T | 2003 | J Neurosei Methods2003,130,: | 1 |
| 14 | Identification of 2 new sesame seed allergens:Ses i6 and Ses i7 Journal of Allergy and Clinical 显示文摘 | Kirsten Beyer M D Galina Grishina M S Ludmilla Bardina MS | 2007 | Immunology2007,119,6: | 1 |
| 15 | Myosin light chain is a novel shrimp allergen, Lit v 3 显示文摘 | Rosalia A Galina G Ludmilla B Teresa C Carlos B Maria D I Sampson H A Kirsten B | 2008 | Journal of Allergy and Clinical Immunology2008,122,4: | 1 |
| 16 | The fingerprints of the host innate immunity on the cells of primary virus-induced tumor显示文摘 | Galina D Natalya Lidia K | 2001 | Immunology Letters2001,75,13: | 1 |
| 17 | Stratigraphy of Upper Permian and Lower Triassic Strata of the Ziri Area ( Slovenia ) 显示文摘 | JURKOVSEK T K JURKOVSEK B ALJINOVIC D GALINA P | 2011 | Geologija2011,542,: | 1 |
| 18 | Effect of goat milk, cow milk, cow milk replacer and partial substitution of the replacer mixture with whey on artificial feeding of female kids 显示文摘 | Galina M A Palma J M Pacheco D | 1995 | Small Ruminant Res1995,17,2: | 1 |
| 19 | The fingerprints of the host in- nate immunity on the cells of primary virus - induced tumor 显示文摘 | Galina D Natalya Lidia K | 2001 | Immunology tters2001,75,13: | 1 |
| 20 | Anti-thymocyte globulin overcomes the negative impact of HLA mismatching in transplantation from unrelated donors显示文摘 | FRANCIS A GALINA D TATJANA Z | 2008 | Exp Hematol2008,36,: | 1 |