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| 1 | Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro显示文摘Amelogenin(AMG) is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG(rhAMG) on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts(OCCM-30)were treated with different concentrations(0.1, 1, 10, 100, 1 000, 10 000, 100 000 ng · mL^(-1)) of recombinant human AMG(rhAMG)and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein(BSP), osteocalcin(OCN), collagen type I(COL I), osteopontin(OPN), runt-related transcription factor 2(Runx2), cementum attachment protein(CAP), and alkaline phosphatase(ALP) genes using quantitative RT-PCR. The dose response of rhAMG was evaluated using a real-time cell analyzer.Total RNA was isolated on day 3, and cell mineralization was assessed using von Kossa staining on day 8. COL I, OPN and lysosomalassociated membrane protein-1(LAMP-1), which is a cell surface binding site for amelogenin, were evaluated using immunocytochemistry. F-actin bundles were imaged using confocal microscopy. rhAMG at a concentration of 100 000 ng · mL^(-1) increased cell proliferation after 72 h compared to the other concentrations and the untreated control group. rhAMG(100 000 ng · mL^(-1)) upregulated BSP and OCN mRNA expression levels eightfold and fivefold, respectively. rhAMG at a concentration of 100 000 ng · mL^(-1) remarkably enhanced LAMP-1 staining in cementoblasts. Increased numbers of mineralized nodules were observed at concentrations of 10 000 and 100 000 ng · mL^(-1) rhAMG. The present data suggest that rhAMG is a potent regulator of gene expression in cementoblasts and support the potential application of rhAMG in therapies aimed at fast regeneration of damaged periodontal tissue. | Sema S.Hakki S.Buket Bozkurt Emre Türkay Michel Dard Nuhan Purali Werner Gotz | 2018 | International Journal of Oral Science2018，10，2： | 3 |
| 2 | Expression of a bacterial carotene hydroxylase gene (crtZ) enhances UV tolerance in tobacco 显示文摘 | Gotz T Sandmann G Romer S | 2002 | Plant Mol Biol2002，50，： | 1 |
| 3 | Reaction kinetics and chemical equilibrium of homogeneously and heterogeneously catalyzed acetic acid esterification with methanol and methyl acetate hydrolysis 显示文摘 | Popken T Gotze L Gmehling J | 2000 | Ind Eng Chem Res2000，39，： | 1 |
| 4 | The very-long-chain fatty acid synthase is inhibited by chloroacetamides显示文摘 | Gotz T Boger P | 2004 | Zeitschrift Fur Naturforschung C-A Journal of Biosciences2004，59，78： | 1 |
| 5 | Chromosomal aberrations in humans as genetic endpoints to assess the impact of pollution 显示文摘 | Huttner E Gotze A Nikolova T | 1999 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis1999，445，2： | 1 |
| 6 | Optimal control of filmcasting processes显示文摘 | Selvanayagam K Gotz T Sundar S | 2009 | International Journal for Numerical Methods in Fluids2009，59，10： | 1 |
| 7 | FGF5 as a regulator of the hair growth cycle: evidence from targeted and spontaneous mutations显示文摘 | HEBERT J M ROSENQUIST T GOTZ J | 1994 | Cell1994，78，6： | 1 |
| 8 | The novel roles of glial cells revisited:the contribution of radial glia and astrocytes to neurogenesis 显示文摘 | Mori T Buffo A Gotz M | 2005 | Curr Top Dev Biol2005，69，： | 1 |
| 9 | Interaction of 1 - allyl-3-methyl-imidazolium chloride and carbonblack and its influence on carbon black filled rubbers显示文摘 | Kreyenschulte H Richter S Gotze T | 2012 | Carbon2012，50，10： | 1 |
| 10 | FGF5 as a regulator of the hair growth cycle: evidence from targeted and spontaneous mutations 显示文摘 | Hebert J M Rosenquist T Gotz J and GR Martin | 1994 | Cell1994，78，6： | 1 |
| 11 | MR imaging of the intraarticular disk of the acromioclavicular joint: a comparison with anatomical, histological and in vivo findings显示文摘 | Heers G Gotz J Schubert T | 2007 | Skeletal Radiol2007，36，1： | 1 |
| 12 | Minimally invasive PCNL in patients with renal pelvic and calyceal stones显示文摘 | Bichler KH Strohmaier WL Gotz T | 2001 | Eur Urol2001，40，6： | 1 |
| 13 | Reactive kinetics and chemical equilibrium of homogenously and methyl acetate hydrolysis 显示文摘 | Popken T Gotze L Gmehling J | 2000 | Ind Eng Chem Res2000，39，： | 1 |
| 14 | FGF5 as a regulator of the hair growth cycle: evidence from targeted and spontaneous mutations显示文摘 | Hebert JM Rosenquist T Gotz J | 1994 | Cell1994，78，6： | 1 |
| 15 | Kavalactones protect neural cells against amyloid beta peptide-induced neurotoxicity via extracellular signal-regulated kinase 1/2-dependent nuclear factor erythroid 2-related factor 2 activation显示文摘 | Wruck C J Gotz ME Herdegen T | | 0，，06： | 1 |
| 16 | Simulation of the fiber spinning process显示文摘 | GOTZ T RAVE H REINEL Bitzer D | 2001 | Berichte des Fraunhofer ITWM2001，26，6： | 1 |
| 17 | The very-longchain fatty acid synthase is inhibited by chloroacetamides显示文摘 | GOTZ T BOGER P | 2004 | Zeitschrift Fur Natur-forschung C-A Journal of Biosciences2004，59，7： | 1 |
| 18 | FGF5 as aregnlator of the hair growth cycle:evidence from targeted and spontaneous mutations显示文摘 | Hebert J M Rosenquist T Gotz J | 1994 | Cell1994，，78： | 1 |
| 19 | FGF5 as a regulator of the hair growth cycle: evidence from targeted and spontaneous mutations显示文摘 | Hebert J M Rosenquist T Gotz J | 1994 | Cell1994，78，： | 1 |
| 20 | Inter- species compatibility of the anther specific cell wall inver- tase promoters from Arabidopsis and tobacco for genera- ting male sterile plants显示文摘 | Hirsche J Engelke T Voller D GOtz M Roitsch T | 2009 | Theor Appl Genet2009，118，2： | 1 |
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