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2篇 您的检索式:作者名="GUOYunchang"
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1Direct measurement of compression spring constant of single DNA molecule with AFM显示文摘In this paper, a new approach is demonstrated to measure the compression elasticity of single biomolecule in small force regime (<0.5 nN) using vibrating mode scanning polarization force microscopy (VSPFM). With this method we investigate the compression elasticity of a single DNA molecule in the radial direction (perpendicular to DNA strands). The radial deformation of DNA molecules deposited on mica surface is shown to be able to reach about 50% un der external load, and this remarkable deformation is re- versible. In addition, the compression spring constant of DNA molecules is estimated to be about 0.6 nN/nm according to the height-force curves.ZHOUXinfei ANHongjie GUOYunchang SUNJielin LIMinqian HUJun 2005Chinese Science Bulletin2005,50,10:2
2Height measurement of DNA molecules with lift mode AFM显示文摘The height of double-stranded DNA (dsDNA) is measured by lift mode AFM combined with conventional tapping mode AFM. While the tip scan height is raised step by step, the tip pressure on sample is decreased gradually. As a result, the deformation of the DNA strands decreases, and the height of double-stranded DNA (dsDNA) molecule can be deduced by the tip lift height. The measured height of dsDNA is 1.5±0.2 nm in lift mode, but only 0.8±0.2 nm in conventional tapping mode. This demonstrates that the tip pressure is a key factor in soft sample height measurement resulting in artificating lower values via conventional tap- ping mode.GUOYunchang ZHOUXingfei SUNJielin LIMinqian HUJun 2004Chinese Science Bulletin2004,49,15:1
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