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		题名	作者	年代	出处	被引量
	1	Molecular architecture of mouse and human pancreatic zymogen granules: protein components and their copy numbers显示文摘A molecular model of pancreatic zymogen granule (ZG) is critical for understanding its functions. We haveextensively characterized the composition and membrane topology of rat ZG proteins. In this study, wereport the development of targeted proteomics approaches to quantify representative mouse and humanZG proteins using LC-SRM and heavy isotope-labeled synthetic peptides. The absolute quantities of mouseRab3D and VAMP8 were determined as 1242 ± 218 and 2039±151 (mean ± SEM) copies per ZG. Thesize distribution and the averaged diameter of ZGs 750 ±23 nm (mean ± SEM) were determined byatomic force microscopy. The absolute quantification of Rab3 D was then validated using semi-quantitativeWestern blotting with purified GST-Rab3D proteins as an internal standard. To extend our proteomicsanalysis to human pancreas, ZGs were purified using human acini obtained from pancreatic islet trans-plantation center. One hundred and eighty human ZG proteins were identified for the first time includingboth the membrane and the content proteins. Furthermore, the copy number per ZG of human Rab3 D andVAMP8 were determined to be 1182 ± 45 and 485 ± 15 (mean ± SEM). The comprehensive proteomicanalyses of mouse and human pancreatic ZGs have the potential to identify species-specific ZG proteins.The determination of protein copy numbers on pancreatic ZGs represents a significant advance towardsbuilding a quantitative molecular model of a prototypical secretory vesicle using targeted proteomicsapproaches. The identification of human ZG proteins lays a foundation for subsequent studies of altered ZGcompositions and secretion in pancreatic diseases.	Jin-sook Lee Joseph A. Caruso Garrett Hubbs Patricia Schnepp Jlames Woods Jingye Fang Chunying Li Kezhong ghang Paul M. Stemmer Bhanu P. Jena Xuequn Chen	2018	Biophysics Reports2018，4，2：	2

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