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您的检索式：作者名="Geert Wiegertjes"
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| 1 | 黄芪对鲤头肾中巨噬细胞的增殖和一氧化氮产量的影响:离体研究(英文)显示文摘用密度梯度离心分离鲤头肾中的巨噬细胞和嗜中性粒细胞。用RPMI细胞培养液对分离的细胞进行原代培养,在细胞培养液中加入不同浓度的黄芪水提取液来研究黄芪对鲤头肾中免疫细胞非特异性免疫应答的影响。黄芪水提取液单独使用时能刺激鲤头肾中巨噬细胞的增殖,但不能刺激巨噬细胞的氮暴发活性。用黄芪和脂多糖(LPS)混合刺激巨噬细胞和中性粒细胞时,黄芪水提取液能显著提高脂多糖刺激所产生的一氧化氮的产量。研究结果表明,黄芪不仅能刺激巨噬细胞数量的增加,而且能协同脂多糖增强头肾中免疫细胞的功能,从而对机体的非特异性免疫起调节作用。 | 殷国俊 Geert Wiegertjes LI Yue-ming Johan Schrama Johan Verreth 徐跑 周洪琪 | 2004 | 水产学报2004，28，6： | 14 |
| 2 | Environmental microbes determine macrophage response towards saponin-induced inflammation in zebrafish larvae显示文摘The microbial consortium within an organism is crucial for its development and immune status.Alteration of the host microbiome by antibiotics or antinutritional factors may contribute to increased disease susceptibility.Here,we investigated whether exposure to different microbes could influence zebrafish larval microbiota composition and modulate their immune response towards a saponin challenge.Adult zebrafish were exposed to the antibiotic oxytetracycline(OxyT)or control tank water and their intestinal content was harvested after 30h(24h exposure,6h wash-out).Subsequently,zebrafish embryos were exposed to either OxyT-treated content or non-treated content from 3 to 6 days post fertilization(dpf).At 6 dpf part of the group received a saponin challenge until 8 dpf.Zebrafish larvae exposed to OxyT-treated adult gut content(3–6 dpf)showed an altered microbiota composition compared to controls.Interestingly,larvae exposed to saponin-treated OxyT-content showed fewer macrophages(as visualized by fluorescent microscopy using mpx:GFP114;mpeg:mCherry transgenic fish)in the overall fish as well as around the gut area than saponin-treated control-exposed larvae.Fewer macrophages were associated with a decreased expression of interleukin 22(il22)in larvae exposed to saponin-treated OxyT-content compared to controls.Overall,this study shows that exposure to different microbial environments early in life might affect disease susceptibility of larval zebrafish. | Adria Lopez Nadal Evelien Kidess Jos Boekhorst Geert Wiegertjes Sylvia Brugman | 2023 | Water Biology and Security2023，2，3： | 0 |
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